##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309572
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.584788029925186	34.0	31.0	34.0	31.0	34.0
2	32.75764927060587	34.0	31.0	34.0	31.0	34.0
3	32.839032599847535	34.0	31.0	34.0	31.0	34.0
4	36.269649709922085	37.0	37.0	37.0	35.0	37.0
5	36.18795304484902	37.0	35.0	37.0	35.0	37.0
6	36.24613014096882	37.0	37.0	37.0	35.0	37.0
7	36.23629074980941	37.0	37.0	37.0	35.0	37.0
8	36.21848552194643	37.0	36.0	37.0	35.0	37.0
9	38.05789606295143	39.0	38.0	39.0	35.0	39.0
10	37.983015259778014	39.0	38.0	39.0	35.0	39.0
11	38.0596081040921	39.0	38.0	39.0	35.0	39.0
12	37.98618092075511	39.0	38.0	39.0	35.0	39.0
13	38.041709198506325	39.0	38.0	39.0	35.0	39.0
14	39.40508185494812	40.0	39.0	41.0	37.0	41.0
15	39.41740855116096	40.0	39.0	41.0	37.0	41.0
16	39.365042704120526	40.0	39.0	41.0	36.0	41.0
17	39.3400242916026	40.0	39.0	41.0	36.0	41.0
18	39.37158722365072	40.0	39.0	41.0	36.0	41.0
19	39.38250875402168	40.0	39.0	41.0	37.0	41.0
20	39.37928494825113	40.0	39.0	41.0	37.0	41.0
21	39.348329306268006	40.0	39.0	41.0	36.0	41.0
22	39.32892832685127	40.0	39.0	41.0	36.0	41.0
23	39.26175816934348	40.0	39.0	41.0	36.0	41.0
24	39.259461450001936	40.0	39.0	41.0	36.0	41.0
25	39.21413435323608	40.0	39.0	41.0	36.0	41.0
26	39.14495497008773	40.0	39.0	41.0	36.0	41.0
27	39.029133771788146	40.0	39.0	41.0	35.0	41.0
28	39.03077797733645	40.0	39.0	41.0	35.0	41.0
29	39.00494553771013	40.0	39.0	41.0	35.0	41.0
30	38.96607574328428	40.0	38.0	41.0	35.0	41.0
31	38.877482459653976	40.0	38.0	41.0	35.0	41.0
32	38.83055638106806	40.0	38.0	41.0	35.0	41.0
33	38.765876758880005	40.0	38.0	41.0	35.0	41.0
34	38.74237334125825	40.0	38.0	41.0	35.0	41.0
35	38.66747638675332	40.0	38.0	41.0	35.0	41.0
36	38.61601501427778	40.0	38.0	41.0	35.0	41.0
37	38.5658522088561	40.0	38.0	41.0	35.0	41.0
38	38.539496466088664	40.0	38.0	41.0	35.0	41.0
39	38.45171720956676	40.0	38.0	41.0	34.0	41.0
40	38.132889925445454	40.0	37.0	41.0	34.0	41.0
41	38.20382980372902	40.0	37.0	41.0	34.0	41.0
42	38.19197795666275	40.0	37.0	41.0	34.0	41.0
43	37.72922938767072	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	3.0
20	6.0
21	26.0
22	48.0
23	81.0
24	160.0
25	247.0
26	412.0
27	660.0
28	1101.0
29	1491.0
30	2067.0
31	2882.0
32	3974.0
33	5373.0
34	7851.0
35	12051.0
36	18175.0
37	32906.0
38	75882.0
39	144174.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.54454537232049	16.218521054875765	13.26734976031424	27.9695838124895
2	20.75058467820087	19.345418836328868	32.92416626826716	26.979830217203105
3	21.34236946493869	19.533420335172433	29.495561614099465	29.628648585789414
4	15.61801454911943	16.420412698822894	34.01018179938754	33.95139095267014
5	17.12041140671637	32.40538549998062	33.38835553603039	17.085847557272622
6	33.352822606695696	33.17128164045844	16.25470003747109	17.221195715374776
7	29.94844494980166	27.58679725556575	20.401069864199606	22.063687930432984
8	27.09127440466192	30.195560321992943	21.008036902562246	21.705128370782887
9	28.544248187820603	12.859367126225887	18.259726331838795	40.33665835411471
10	19.50628609822594	23.3034641375835	29.68485521946429	27.50539454472627
11	38.655304743323036	19.654555321540705	18.816624242502552	22.8735156926337
12	22.59054436447741	24.060961585673123	26.995012468827927	26.353481581021537
13	35.27515408370266	17.698306048350627	21.09428501285646	25.932254855090253
14	24.087772795989302	20.83004922925846	23.708862558629335	31.373315416122903
15	29.731694080860027	24.20923080898789	20.27767369141912	25.78140141873296
16	24.874665667437622	24.398524414352718	23.059579031695375	27.667230886514282
17	26.574108769526962	23.788004083056606	22.287868411871877	27.350018735544555
18	26.776646466734714	21.19539234814518	23.80318633468143	28.22477485043867
19	27.836819867429867	22.62543124055147	23.653301978215083	25.884446913803572
20	29.909358727533498	20.833279495561612	23.19201995012469	26.065341826780198
21	27.400087863243446	22.298528290672284	22.466179111805978	27.835204734278296
22	28.31619138681793	22.766270851368986	21.838215342472832	27.07932241934025
23	27.842957373405863	21.959027302210792	22.806326153528097	27.391689170855244
24	27.601333453929943	21.510343312702698	23.720168490690373	27.168154742676986
25	27.614254519142555	21.996498391327382	23.615830889098497	26.773416200431566
26	27.551910379491684	22.87706898556717	22.947811817606244	26.623208817334902
27	26.57540087604822	21.88763841691109	24.819105087023374	26.717855620017318
28	26.02464047136046	22.977853294225575	22.51107981341982	28.48642642099415
29	26.96464796557828	23.289897019110256	22.880299251870323	26.86515576344114
30	26.623531843965214	22.169963691806753	24.25929993668678	26.947204527541253
31	27.823898802217258	22.290129598284082	23.200418642512886	26.685552956985774
32	25.595338079671286	22.282699985786827	23.323491788663056	28.798470145878824
33	25.672864470946983	21.93673846471903	25.2558370912098	27.134559973124183
34	25.840838318710997	21.96904112775057	24.31679867688292	27.87332187665551
35	25.480340599279007	23.65911645756076	24.965436150556254	25.895106792603983
36	25.99653715452302	22.325339500988463	25.195108084710505	26.483015259778014
37	26.2959828408254	22.371209282493247	24.83267220549662	26.500135671184733
38	25.645730234000492	21.5187420050909	25.473880066672695	27.361647694235913
39	25.183479126019147	20.522850903828513	26.792151744989855	27.50151822516248
40	24.633041747961702	21.81980282454486	27.29671934154252	26.250436085950923
41	23.179098884912072	20.695347124416937	28.45670797100513	27.66884601966586
42	21.844029821818513	21.1517837530526	29.376041760882764	27.62814466424612
43	21.17310351065342	22.026216841316398	28.971612419727883	27.8290672283023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	11.0
4	18.0
5	18.0
6	26.0
7	34.0
8	38.0
9	42.0
10	68.0
11	94.0
12	94.0
13	185.0
14	276.0
15	535.5
16	795.0
17	753.0
18	711.0
19	711.0
20	872.5
21	1034.0
22	895.0
23	756.0
24	841.0
25	926.0
26	926.0
27	1061.0
28	1196.0
29	1410.0
30	1624.0
31	1997.0
32	2370.0
33	2370.0
34	3056.5
35	3743.0
36	4106.0
37	4469.0
38	5460.0
39	6451.0
40	6451.0
41	7757.0
42	9063.0
43	10924.5
44	12786.0
45	18604.5
46	24423.0
47	24423.0
48	27283.5
49	30144.0
50	33681.5
51	37219.0
52	37247.0
53	37275.0
54	37275.0
55	33740.0
56	30205.0
57	28459.0
58	26713.0
59	24497.0
60	22281.0
61	22281.0
62	20252.0
63	18223.0
64	15009.0
65	11795.0
66	10214.5
67	8634.0
68	8634.0
69	7253.5
70	5873.0
71	4934.5
72	3996.0
73	3222.5
74	2449.0
75	2449.0
76	2001.0
77	1553.0
78	1274.5
79	996.0
80	794.5
81	593.0
82	593.0
83	491.0
84	389.0
85	285.5
86	182.0
87	167.5
88	153.0
89	153.0
90	104.5
91	56.0
92	38.5
93	21.0
94	11.5
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	309572.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.60275954780207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.50177107171899	46.90336394764176
2	5.933459105198523	6.360995634053464
3	1.8998536330609022	3.055121924069451
4	1.0014144113898875	2.147143036057435
5	0.6888943736401361	1.8463319732032961
6	0.4388452200908306	1.411398888673864
7	0.3543613365859092	1.329632186263657
8	0.2820205357651557	1.2093663172929563
9	0.20954371448257944	1.0108909207946695
>10	1.4100143184017153	14.62870041873306
>50	0.1594686109920728	5.944862594725513
>100	0.11313244855286674	11.63457671889064
>500	0.00722122012039575	2.5176154396002506
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	808	0.26100551729484583	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	791	0.25551406457948395	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	709	0.22902588089362086	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	696	0.22482653469952063	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	657	0.2122284961172199	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	654	0.2112594162262737	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	627	0.2025376972077578	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	624	0.2015686173168116	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	613	0.19801532438334216	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	597	0.1928468982982957	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	517	0.16700476787306345	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	512	0.16538963472148643	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	490	0.15828304885454758	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	452	0.14600803690256225	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	451	0.14568501027224684	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	434	0.140193557556885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.13793237114467718	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	419	0.13534815810215395	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	417	0.13470210484152315	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	405	0.1308257852777383	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	404	0.13050275864742292	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	399	0.1288876254958459	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	391	0.12630341245332266	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	375	0.1211349863682762	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	358	0.11564353365291434	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	357	0.11532050702259894	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	346	0.1117672140891295	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	338	0.10918300104660629	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	335	0.10821392115566007	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	335	0.10821392115566007	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	334	0.10789089452534467	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.10659878800408305	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	327	0.10562970811313685	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	316	0.10207641517966741	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	314	0.10143036191903661	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	314	0.10143036191903661	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	313	0.1011073352887212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.230266303154032E-4	0.0
3	0.0	0.0	0.0	3.230266303154032E-4	0.0
4	0.0	0.0	0.0	3.230266303154032E-4	0.0
5	0.0	0.0	0.0	3.230266303154032E-4	0.0
6	0.0	0.0	0.0	3.230266303154032E-4	0.0
7	0.0	0.0	0.0	6.460532606308064E-4	0.0
8	3.230266303154032E-4	0.0	0.0	6.460532606308064E-4	0.0
9	3.230266303154032E-4	0.0	0.0	6.460532606308064E-4	0.0
10	3.230266303154032E-4	0.0	0.0	9.690798909462096E-4	0.0
11	3.230266303154032E-4	0.0	0.0	9.690798909462096E-4	0.0
12	3.230266303154032E-4	0.0	0.0	9.690798909462096E-4	0.0
13	3.230266303154032E-4	0.0	0.0	9.690798909462096E-4	0.0
14	3.230266303154032E-4	0.0	0.0	9.690798909462096E-4	0.0
15	3.230266303154032E-4	0.0	0.0	0.0012921065212616128	0.0
16	3.230266303154032E-4	0.0	0.0	0.001615133151577016	0.0
17	9.690798909462096E-4	0.0	0.0	0.0022611864122078222	0.0
18	0.0012921065212616128	0.0	0.0	0.0025842130425232255	0.0
19	0.0012921065212616128	0.0	0.0	0.002907239672838629	0.0
20	0.0012921065212616128	0.0	0.0	0.0038763195637848383	0.0
21	0.0012921065212616128	0.0	0.0	0.005491452715361855	0.0
22	0.0012921065212616128	0.0	0.0	0.008398692388200483	0.0
23	0.0012921065212616128	0.0	0.0	0.010659878800408305	0.0
24	0.0012921065212616128	0.0	0.0	0.013244091842931531	0.0
25	0.0012921065212616128	0.0	0.0	0.014859224994508547	0.0
26	0.0012921065212616128	0.0	0.0	0.018735544558293387	0.0
27	0.0012921065212616128	0.0	0.0	0.041670435310687017	0.0
28	0.0012921065212616128	0.0	0.0	0.1308257852777383	0.0
29	0.0012921065212616128	0.0	0.0	0.27005026294367707	0.0
30	0.0012921065212616128	0.0	0.0	0.43931621722894837	0.0
31	0.0012921065212616128	0.0	0.0	0.9364542012843539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGAC	20	0.0018398594	37.0	3
CGATCGC	20	0.0018398594	37.0	19
GGTATCA	550	0.0	31.954546	1
TCCTCCG	25	0.0054902174	29.6	21
GTCTAGA	25	0.0054902174	29.6	1
CCGCTTA	25	0.0054902174	29.6	25
CAGTCGG	165	0.0	24.666666	10
GCAGTCG	170	0.0	23.941177	9
AGTCGGT	170	0.0	23.941177	11
TCTATGG	205	0.0	23.463415	2
TGCTTGG	40	0.0019280182	23.125	2
CTAGTTC	40	0.0019280182	23.125	3
TATACAC	65	2.6723446E-6	22.76923	37
GTATCAA	775	0.0	22.43871	2
CTATGGG	220	0.0	21.863636	3
GATTCCT	180	0.0	21.583332	18
CTCTATG	215	0.0	21.511627	1
GGGCAGT	200	0.0	21.275002	7
TATGGGC	230	0.0	20.913044	4
CTTATTG	170	0.0	20.676472	28
>>END_MODULE