FastQCFastQC Report
Fri 10 Feb 2017
ERR1631755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279369
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118710.5208020289825824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107710.47254305906590816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91740.4024798091050637No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA51090.2241409793675355No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA49960.21918346700336802No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG43620.19136875161503028No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA42000.18426152150002917No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40720.17864593227336162No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT39800.17460972751669432No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA39750.17439036856252763No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC37140.1629398311550258No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA36200.15881588281669182No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA34350.15069960151252385No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT32270.14157426901918907No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA29750.13051857772918735No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28210.12376232194085293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25350.1112149897625176No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA25170.11042529752751748No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA24480.107398143960017No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA24380.10695942605168361No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC24050.10551165695418337No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA23580.10344968278501639No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA22950.10068575996251593No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT22830.10015929847251585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.027.4166661
CTTATAC24450.018.38650337
GCAGTCG10900.018.3302759
CAGTCGG11100.018.010
CTAAGTC2300.017.69565234
CTCTATG15900.017.452831
AGTCGGT11400.017.36403511
TCTATGG16250.017.3046152
GTATCAA70600.017.1111892
TATACAC8050.016.77639837
TAATACT2600.016.3653854
GTGATTC12250.016.31020416
TCTTATA43400.016.19815637
AGAGTAT1151.2434302E-616.0869566
AACGTCA12100.016.05371928
GGGCAGT12800.016.0429697
TAAGGTA3150.015.8571435
GTAACGT12900.015.77519426
ATTAGAG2350.015.7446813
TCGGGTA2250.015.62222225
Produced by FastQC (version 0.11.5)