FastQCFastQC Report
Fri 10 Feb 2017
ERR1631754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80.3833253473885961No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA70.33540967896502155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.33540967896502155No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT70.33540967896502155RNA PCR Primer, Index 46 (95% over 21bp)
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.287494010541447No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT50.23957834211787254No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.23957834211787254No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT50.23957834211787254No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC40.19166267369429804No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40.19166267369429804No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC40.19166267369429804No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG40.19166267369429804No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.19166267369429804No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA40.19166267369429804No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA40.19166267369429804No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA40.19166267369429804No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG30.1437470052707235No Hit
GTATCAACGCAGAGTACGGGATCCTCCAAGGCCTGTCTCTTAT30.1437470052707235No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.1437470052707235No Hit
GAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT30.1437470052707235No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC30.1437470052707235No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA30.1437470052707235No Hit
GGTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCAT30.1437470052707235No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.1437470052707235No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC30.1437470052707235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT30.1437470052707235No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.1437470052707235No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC30.1437470052707235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA30.1437470052707235No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG30.1437470052707235No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA30.1437470052707235No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT30.1437470052707235No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC30.1437470052707235No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCT30.1437470052707235No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30.1437470052707235No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC30.1437470052707235No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30.1437470052707235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)