##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30091039770005	33.0	31.0	34.0	30.0	34.0
2	32.442740776233826	34.0	31.0	34.0	30.0	34.0
3	32.48873981792046	34.0	31.0	34.0	30.0	34.0
4	36.002874940105414	37.0	35.0	37.0	35.0	37.0
5	35.95543842836607	37.0	35.0	37.0	35.0	37.0
6	36.02539530426449	37.0	35.0	37.0	35.0	37.0
7	36.02874940105414	37.0	35.0	37.0	35.0	37.0
8	35.90848107331097	37.0	35.0	37.0	35.0	37.0
9	37.67465261140393	39.0	37.0	39.0	35.0	39.0
10	37.60804983229516	39.0	37.0	39.0	35.0	39.0
11	37.66986104456157	39.0	37.0	39.0	35.0	39.0
12	37.68040249161476	39.0	37.0	39.0	35.0	39.0
13	37.72400574988021	39.0	37.0	39.0	35.0	39.0
14	39.08097747963584	40.0	38.0	41.0	36.0	41.0
15	39.09008145663632	40.0	38.0	41.0	36.0	41.0
16	39.007187350263536	40.0	38.0	41.0	36.0	41.0
17	38.987062769525636	40.0	38.0	41.0	36.0	41.0
18	39.00095831336847	40.0	38.0	41.0	36.0	41.0
19	38.98897939626258	40.0	38.0	41.0	35.0	41.0
20	39.027311931001435	40.0	39.0	41.0	35.0	41.0
21	38.943938667944415	40.0	38.0	41.0	34.0	41.0
22	38.91662673694298	40.0	38.0	41.0	35.0	41.0
23	38.822712026832775	40.0	38.0	41.0	35.0	41.0
24	38.73981792045999	40.0	38.0	41.0	35.0	41.0
25	38.776233828461905	40.0	38.0	41.0	35.0	41.0
26	38.5764254911356	40.0	38.0	41.0	34.0	41.0
27	38.47244849065645	40.0	38.0	41.0	34.0	41.0
28	38.36559655007187	40.0	38.0	41.0	34.0	41.0
29	38.347388596070914	40.0	38.0	41.0	34.0	41.0
30	38.40249161475803	40.0	38.0	41.0	34.0	41.0
31	38.29659798754193	40.0	38.0	41.0	34.0	41.0
32	38.241494968854816	40.0	38.0	41.0	34.0	41.0
33	38.07570675610925	40.0	37.0	41.0	33.0	41.0
34	38.046957355055106	40.0	37.0	41.0	33.0	41.0
35	37.88979396262578	40.0	37.0	41.0	33.0	41.0
36	37.87973167225683	40.0	37.0	41.0	33.0	41.0
37	37.75994250119789	40.0	37.0	41.0	33.0	41.0
38	37.80881648298994	40.0	36.0	41.0	33.0	41.0
39	37.76090081456636	40.0	37.0	41.0	33.0	41.0
40	37.4168663152851	40.0	36.0	41.0	32.0	41.0
41	37.42788691902252	40.0	36.0	41.0	32.0	41.0
42	37.387158600862485	39.0	36.0	41.0	33.0	41.0
43	36.89027311931002	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	1.0
25	4.0
26	8.0
27	6.0
28	13.0
29	12.0
30	15.0
31	42.0
32	50.0
33	66.0
34	71.0
35	106.0
36	147.0
37	252.0
38	458.0
39	834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36367992333493	16.722568279827506	13.560134163871588	26.35361763296598
2	21.849544801149975	20.028749401054142	31.43267848586488	26.689027311931003
3	20.07666506947772	20.26832774317202	30.09103977000479	29.56396741734547
4	15.333013895543843	15.141351221849545	35.26593195975084	34.25970292285577
5	16.866315285098228	34.40344992812649	32.39099185433637	16.339242932438907
6	32.199329180642074	33.013895543842835	15.572592237661715	19.214183037853378
7	29.516051748921896	27.599425011978916	21.274556780067083	21.609966459032105
8	26.25778629611883	31.816003833253475	20.555821753713463	21.370388116914228
9	28.79731672256828	14.03929084810733	17.201724964063246	39.961667465261144
10	22.041207474844274	22.52036415908002	29.65979875419262	25.778629611883087
11	37.997125059894586	20.795400095831337	17.105893627216098	24.101581217057976
12	23.239099185433638	25.49113560134164	24.293243890752276	26.976521322472447
13	34.11595591758505	17.297556300910397	20.795400095831337	27.791087685673215
14	24.484906564446575	20.93914710110206	24.149496885481554	30.426449448969812
15	31.384762817441302	22.472448490656443	19.549592716818402	26.59319597508385
16	27.168183996166746	22.85577383804504	20.891231432678488	29.084810733109727
17	26.401533301389556	23.57450886439866	22.61619549592717	27.40776233828462
18	27.264015333013898	21.274556780067083	22.472448490656443	28.988979396262575
19	30.761859127934837	21.753713464302827	23.862002874940107	23.622424532822233
20	30.09103977000479	20.172496406324868	23.095352180162912	26.641111643507426
21	27.359846669861042	21.226641111643506	21.897460469573552	29.516051748921896
22	28.27024436990896	21.849544801149975	22.903689506468616	26.976521322472447
23	27.21609966459032	22.137038811691422	21.705797795879253	28.941063727839005
24	27.0723526593196	21.130809774796358	24.149496885481554	27.647340680402493
25	27.599425011978916	22.80785816962147	23.670340201245807	25.92237661715381
26	27.311931001437472	22.376617153809296	24.916147580258745	25.395304264494488
27	26.449448969813126	21.65788212745568	25.299472927647344	26.59319597508385
28	27.120268327743176	23.38284619070436	21.705797795879253	27.791087685673215
29	26.976521322472447	23.430761859127934	23.430761859127934	26.161954959271682
30	25.634882606612365	22.089123143267848	24.628653569717297	27.647340680402493
31	27.599425011978916	22.232870148538574	22.712026832774317	27.455678006708194
32	24.34115955917585	22.903689506468616	23.430761859127934	29.324389075227597
33	25.155725922376615	22.85577383804504	24.53282223287015	27.455678006708194
34	26.161954959271682	22.75994250119789	24.67656923814087	26.401533301389556
35	23.862002874940107	23.478677527551508	26.449448969813126	26.20987062769526
36	25.73071394345951	24.389075227599424	24.96406324868232	24.916147580258745
37	26.06612362242453	21.8016291327264	25.73071394345951	26.401533301389556
38	24.053665548634402	22.232870148538574	25.970292285577383	27.74317201724964
39	22.56827982750359	20.50790608528989	27.455678006708194	29.468136080498326
40	25.443219932918065	22.472448490656443	26.20987062769526	25.87446094873023
41	22.472448490656443	20.84331576425491	28.27024436990896	28.41399137517968
42	21.849544801149975	20.459990416866315	30.234786775275513	27.455678006708194
43	20.987062769525636	20.891231432678488	29.75563009103977	28.36607570675611
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	3.0
16	6.0
17	10.5
18	15.0
19	15.0
20	16.5
21	18.0
22	10.0
23	2.0
24	5.0
25	8.0
26	8.0
27	10.0
28	12.0
29	12.0
30	12.0
31	16.0
32	20.0
33	20.0
34	24.5
35	29.0
36	27.0
37	25.0
38	37.5
39	50.0
40	50.0
41	60.5
42	71.0
43	79.0
44	87.0
45	115.0
46	143.0
47	143.0
48	161.5
49	180.0
50	198.5
51	217.0
52	223.5
53	230.0
54	230.0
55	223.0
56	216.0
57	200.5
58	185.0
59	164.5
60	144.0
61	144.0
62	131.5
63	119.0
64	101.5
65	84.0
66	76.5
67	69.0
68	69.0
69	60.5
70	52.0
71	45.5
72	39.0
73	28.0
74	17.0
75	17.0
76	15.5
77	14.0
78	10.0
79	6.0
80	6.0
81	6.0
82	6.0
83	5.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2087.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.36895064686152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.10710498409331	84.13991375179684
2	4.931071049840933	8.912314326784859
3	1.1134676564156947	3.0186871106851942
4	0.4241781548250266	1.5333013895543843
5	0.15906680805938495	0.7187350263536176
6	0.05302226935312832	0.287494010541447
7	0.15906680805938495	1.0062290368950648
8	0.05302226935312832	0.3833253473885961
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.3833253473885961	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	7	0.33540967896502155	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.33540967896502155	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7	0.33540967896502155	RNA PCR Primer, Index 46 (95% over 21bp)
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.287494010541447	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	5	0.23957834211787254	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	5	0.23957834211787254	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT	5	0.23957834211787254	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	4	0.19166267369429804	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	4	0.19166267369429804	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	4	0.19166267369429804	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	4	0.19166267369429804	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.19166267369429804	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	4	0.19166267369429804	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	4	0.19166267369429804	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	4	0.19166267369429804	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	3	0.1437470052707235	No Hit
GTATCAACGCAGAGTACGGGATCCTCCAAGGCCTGTCTCTTAT	3	0.1437470052707235	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3	0.1437470052707235	No Hit
GAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT	3	0.1437470052707235	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	3	0.1437470052707235	No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA	3	0.1437470052707235	No Hit
GGTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCAT	3	0.1437470052707235	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	3	0.1437470052707235	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	3	0.1437470052707235	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	3	0.1437470052707235	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	3	0.1437470052707235	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	3	0.1437470052707235	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3	0.1437470052707235	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	3	0.1437470052707235	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	3	0.1437470052707235	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	3	0.1437470052707235	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	3	0.1437470052707235	No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCT	3	0.1437470052707235	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	3	0.1437470052707235	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	3	0.1437470052707235	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	3	0.1437470052707235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.09583133684714902	0.0
8	0.0	0.0	0.0	0.09583133684714902	0.0
9	0.0	0.0	0.0	0.09583133684714902	0.0
10	0.0	0.0	0.0	0.09583133684714902	0.0
11	0.0	0.0	0.0	0.09583133684714902	0.0
12	0.0	0.0	0.0	0.09583133684714902	0.0
13	0.0	0.0	0.0	0.09583133684714902	0.0
14	0.0	0.0	0.0	0.09583133684714902	0.0
15	0.0	0.0	0.0	0.09583133684714902	0.0
16	0.0	0.0	0.0	0.09583133684714902	0.0
17	0.0	0.0	0.0	0.09583133684714902	0.0
18	0.0	0.0	0.0	0.09583133684714902	0.0
19	0.0	0.0	0.0	0.09583133684714902	0.0
20	0.0	0.0	0.0	0.09583133684714902	0.0
21	0.0	0.0	0.0	0.09583133684714902	0.0
22	0.0	0.0	0.0	0.09583133684714902	0.0
23	0.0	0.0	0.0	0.09583133684714902	0.0
24	0.0	0.0	0.0	0.09583133684714902	0.0
25	0.0	0.0	0.0	0.09583133684714902	0.0
26	0.0	0.0	0.0	0.14374700527072354	0.0
27	0.0	0.0	0.0	0.19166267369429804	0.0
28	0.0	0.0	0.0	0.23957834211787254	0.0
29	0.0	0.0	0.0	0.2874940105414471	0.0
30	0.0	0.0	0.0	0.3833253473885961	0.0
31	0.0	0.0	0.0	0.7187350263536176	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE