FastQCFastQC Report
Fri 10 Feb 2017
ERR1631753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857316
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32300.37675722837320197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31760.3704585007161887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25360.2958069136701053No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20120.23468592677612457No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20020.23351949572852948No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18460.21532317138604667No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17940.2092577299385524No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17610.20540850748148876No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17390.20284235917677965No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17260.20132599881490607No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16620.19386084011029772No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15690.18301303136766373No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC15450.1802135968534356No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13300.15513532933014196No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13060.15233589481591384No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11760.13717229119717816No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11390.1328564963210765No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10760.12550798072122765No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10510.12259190310224001No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10350.12072561342608794No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10330.12049232721656891No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC10320.1203756841118094No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10060.11734296338806229No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9790.11419359955955563No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9630.11232730988340354No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9570.11162745125484652No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9570.11162745125484652No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC9430.10999444778821345No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9420.10987780468345394No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9380.10941123226441592No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9350.1090613029501374No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9180.10707837016922582No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8950.10439557875975719No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8830.10299586150264313No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8780.1024126459788456No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8780.1024126459788456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.029.2965321
ATACGGC1250.023.6829
GACGTAT551.9019277E-523.5454541
TCTAGAC400.001930788723.1250023
ACGTATA603.724864E-521.5833342
TTTTACG555.141629E-420.1818184
GTTAGGT656.8995134E-519.92307720
ATTTCGG656.8995134E-519.92307729
ATACACA2050.019.85365737
CTTATAC12150.019.64197537
GACCGTT1052.2573658E-819.3809537
GTACTGG5800.018.8189661
GTATCAA24550.018.7637482
TATGCGG609.234204E-418.55
TACTGCG500.007033461218.4999987
TACTGGT6000.018.1916682
TATACAC4100.018.0487837
GCGGTAA6950.017.83453223
GTGTATA1054.7937465E-717.6190510
TTTCGGT752.0664943E-417.26666630
Produced by FastQC (version 0.11.5)