##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631753.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857316
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61243695440188	34.0	31.0	34.0	31.0	34.0
2	32.76858008015714	34.0	31.0	34.0	31.0	34.0
3	32.859527875369174	34.0	31.0	34.0	31.0	34.0
4	36.28446453816329	37.0	37.0	37.0	35.0	37.0
5	36.205147226926826	37.0	35.0	37.0	35.0	37.0
6	36.26027625752931	37.0	37.0	37.0	35.0	37.0
7	36.25307704510355	37.0	37.0	37.0	35.0	37.0
8	36.236748176868275	37.0	37.0	37.0	35.0	37.0
9	38.06162955083073	39.0	38.0	39.0	35.0	39.0
10	38.00389354683687	39.0	38.0	39.0	35.0	39.0
11	38.071494058200244	39.0	38.0	39.0	37.0	39.0
12	38.01379304713781	39.0	38.0	39.0	35.0	39.0
13	38.07350381889525	39.0	38.0	39.0	37.0	39.0
14	39.43684125806587	40.0	39.0	41.0	37.0	41.0
15	39.44919142999781	40.0	39.0	41.0	37.0	41.0
16	39.38555795062731	40.0	39.0	41.0	37.0	41.0
17	39.37653793933625	40.0	39.0	41.0	37.0	41.0
18	39.41148537995325	40.0	39.0	41.0	37.0	41.0
19	39.43009462088658	41.0	39.0	41.0	37.0	41.0
20	39.42883954107937	41.0	39.0	41.0	37.0	41.0
21	39.3967895151846	40.0	39.0	41.0	37.0	41.0
22	39.36273206145692	40.0	39.0	41.0	36.0	41.0
23	39.30838453965632	40.0	39.0	41.0	36.0	41.0
24	39.3155989156857	40.0	39.0	41.0	36.0	41.0
25	39.26578181207396	40.0	39.0	41.0	36.0	41.0
26	39.19679907991919	40.0	39.0	41.0	36.0	41.0
27	39.076591361878236	40.0	39.0	41.0	35.0	41.0
28	39.07166785642634	40.0	39.0	41.0	35.0	41.0
29	39.04150045024238	40.0	39.0	41.0	35.0	41.0
30	38.98795076727834	40.0	38.0	41.0	35.0	41.0
31	38.88125965221692	40.0	38.0	41.0	35.0	41.0
32	38.82893472185285	40.0	38.0	41.0	35.0	41.0
33	38.75278777020375	40.0	38.0	41.0	35.0	41.0
34	38.71902892282426	40.0	38.0	41.0	35.0	41.0
35	38.63543547536731	40.0	38.0	41.0	35.0	41.0
36	38.58376957854514	40.0	38.0	41.0	35.0	41.0
37	38.52430492373874	40.0	38.0	41.0	35.0	41.0
38	38.479228195904426	40.0	38.0	41.0	35.0	41.0
39	38.38720145197337	40.0	38.0	41.0	34.0	41.0
40	38.04146312444886	40.0	37.0	41.0	34.0	41.0
41	38.08994116521796	40.0	37.0	41.0	34.0	41.0
42	38.05294197238825	40.0	37.0	41.0	34.0	41.0
43	37.5751029958615	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	3.0
15	2.0
16	1.0
17	2.0
18	1.0
19	10.0
20	25.0
21	50.0
22	115.0
23	198.0
24	407.0
25	688.0
26	1145.0
27	1898.0
28	2928.0
29	4411.0
30	6210.0
31	8213.0
32	11123.0
33	15129.0
34	21670.0
35	32619.0
36	49489.0
37	92283.0
38	202835.0
39	405859.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37292200308871	16.578134550154203	13.106602466301807	26.94234098045528
2	20.534085448072823	19.853472931801107	32.630791913366835	26.981649706759235
3	21.6425448726024	20.31584619906779	29.417740949661503	28.62386797866831
4	15.139575139155223	16.54862384465005	34.670996458715344	33.640804557479385
5	16.796257156054477	32.8089059343346	33.60219568980399	16.792641219806935
6	32.583668099044	33.98338535615806	16.187380149209858	17.245566395588092
7	29.409459289223577	28.123935631669067	20.674407103098506	21.792197976008847
8	27.439240606730774	29.96724661618353	20.731328938221147	21.86218383886455
9	28.141198811173478	13.345837474163552	18.798902621670422	39.714061092992544
10	20.05153292368275	23.777346975910866	29.320927172711116	26.85019292769527
11	37.76297187968031	20.50877389434001	19.27119055284166	22.457063673138027
12	22.930401392252097	24.460525640487287	26.781256852782402	25.827816114478207
13	35.029790648955576	18.05390311157146	21.37648195064597	25.53982428882699
14	23.69837959398868	21.027835710519806	24.386107339650724	30.887677355840786
15	29.38811360105259	24.63467379589323	21.01570482762482	24.961507775429364
16	24.21429204633997	24.50729952549585	23.96024336417377	27.318165063990406
17	26.058419532587752	24.106980389961226	22.8212234461972	27.01337663125382
18	25.9859841645321	21.44915060491114	24.7502671127099	27.814598117846863
19	27.701221020020622	22.699914617247316	25.039075440094432	24.559788922637626
20	29.394762258023878	20.931371862883697	23.897139444498876	25.776726434593545
21	26.627987813128414	22.565891689878644	23.809190543510212	26.996929953482727
22	27.456387143130424	23.189932300341997	23.31917286041553	26.03450769611205
23	27.20198853164994	22.240924000018662	23.96012672106901	26.596960747262386
24	26.721302296936017	22.034698990803857	24.834133505031982	26.40986520722814
25	26.79443752362023	22.711578927723266	24.59909764894158	25.894885899714925
26	26.746963779983112	23.342034908948392	24.062656010152615	25.84834530091588
27	26.087113736358592	22.039947930518036	25.84391286293502	26.029025470188355
28	25.444643515343234	23.62361136383784	23.436165894489314	27.495579226329614
29	26.425728669475433	24.036178025372205	23.872761035604142	25.66533226954822
30	26.509478418692755	22.115416019297435	24.933746716496604	26.441358845513207
31	26.351660297953146	23.26236766839765	24.494002211553266	25.891969822095938
32	24.513015037629067	22.69163295680939	24.550457474256866	28.244894531304677
33	24.371876880870065	22.66550490134326	26.350727153115074	26.611891064671607
34	25.666848629910096	22.056744537603404	25.4055680752488	26.870838757237703
35	24.6840138292065	23.612880198199964	25.64911887798665	26.05398709460689
36	24.95742526676278	22.81714093753062	26.375338848219325	25.850094947487275
37	25.415482739153354	22.24127392933294	26.114291579767553	26.22895175174615
38	24.920449402554016	21.726877837343523	26.392368741514215	26.960304018588243
39	24.421450200392854	20.75582399022064	27.860905430436382	26.96182037895012
40	24.01168297337271	21.793131120846922	28.146097821573374	26.04908808420699
41	22.20546449617177	21.2461916026296	29.298998269016323	27.249345632182298
42	20.9356876577598	21.97019535387185	30.0030560493447	27.09106093902365
43	20.80131480107685	22.867414115681967	29.024303757307692	27.306967325933496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	42.5
2	45.0
3	107.0
4	169.0
5	169.0
6	223.5
7	278.0
8	292.5
9	307.0
10	473.5
11	640.0
12	640.0
13	1393.5
14	2147.0
15	3902.0
16	5657.0
17	5443.0
18	5229.0
19	5229.0
20	5895.5
21	6562.0
22	5132.5
23	3703.0
24	3663.0
25	3623.0
26	3623.0
27	3808.0
28	3993.0
29	4549.5
30	5106.0
31	6131.0
32	7156.0
33	7156.0
34	9178.5
35	11201.0
36	12350.0
37	13499.0
38	16564.5
39	19630.0
40	19630.0
41	23769.0
42	27908.0
43	33514.0
44	39120.0
45	54050.5
46	68981.0
47	68981.0
48	77093.0
49	85205.0
50	94329.0
51	103453.0
52	100407.5
53	97362.0
54	97362.0
55	86361.5
56	75361.0
57	70799.5
58	66238.0
59	62362.0
60	58486.0
61	58486.0
62	53083.0
63	47680.0
64	40401.5
65	33123.0
66	28393.0
67	23663.0
68	23663.0
69	21028.5
70	18394.0
71	14880.5
72	11367.0
73	8103.0
74	4839.0
75	4839.0
76	3858.0
77	2877.0
78	2266.0
79	1655.0
80	1392.0
81	1129.0
82	1129.0
83	909.0
84	689.0
85	547.0
86	405.0
87	322.5
88	240.0
89	240.0
90	178.5
91	117.0
92	68.0
93	19.0
94	16.5
95	14.0
96	14.0
97	9.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	857316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.067048035130846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.16762142271752	32.04012887701296
2	7.04868870302789	5.366455428856936
3	2.433504297935103	2.7790897500957876
4	1.2837663168000475	1.9547677619004162
5	0.8287696852598232	1.577440770942298
6	0.588592982792822	1.3443598409469177
7	0.439964342030337	1.1723700619269501
8	0.32557766804204485	0.9915024582817924
9	0.27756626333945966	0.950951545152749
>10	2.043445758258735	15.657117559614123
>50	0.2855687431787941	7.599552667378977
>100	0.24794519884226182	18.37040763875872
>500	0.021895658106716814	5.760445364550013
>1k	0.0070929596683730525	4.435410274581275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3230	0.37675722837320197	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3176	0.3704585007161887	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2536	0.2958069136701053	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2012	0.23468592677612457	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2002	0.23351949572852948	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1846	0.21532317138604667	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1794	0.2092577299385524	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1761	0.20540850748148876	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1739	0.20284235917677965	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1726	0.20132599881490607	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1662	0.19386084011029772	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1569	0.18301303136766373	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1545	0.1802135968534356	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1330	0.15513532933014196	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1306	0.15233589481591384	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1176	0.13717229119717816	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1139	0.1328564963210765	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1076	0.12550798072122765	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1051	0.12259190310224001	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1035	0.12072561342608794	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1033	0.12049232721656891	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1032	0.1203756841118094	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1006	0.11734296338806229	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	979	0.11419359955955563	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	963	0.11232730988340354	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	957	0.11162745125484652	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	957	0.11162745125484652	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	943	0.10999444778821345	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	942	0.10987780468345394	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	938	0.10941123226441592	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	935	0.1090613029501374	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	918	0.10707837016922582	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	895	0.10439557875975719	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	883	0.10299586150264313	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	878	0.1024126459788456	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	878	0.1024126459788456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1664310475950524E-4
2	0.0	0.0	0.0	0.0	1.1664310475950524E-4
3	0.0	0.0	0.0	0.0	1.1664310475950524E-4
4	0.0	0.0	0.0	0.0	1.1664310475950524E-4
5	0.0	0.0	0.0	0.0	1.1664310475950524E-4
6	0.0	0.0	0.0	0.0	1.1664310475950524E-4
7	0.0	0.0	0.0	0.0	1.1664310475950524E-4
8	0.0	0.0	0.0	0.0	1.1664310475950524E-4
9	0.0	0.0	0.0	0.0	1.1664310475950524E-4
10	0.0	0.0	0.0	0.0	1.1664310475950524E-4
11	0.0	0.0	0.0	1.1664310475950524E-4	1.1664310475950524E-4
12	0.0	0.0	0.0	1.1664310475950524E-4	1.1664310475950524E-4
13	0.0	0.0	0.0	1.1664310475950524E-4	1.1664310475950524E-4
14	0.0	0.0	0.0	3.499293142785157E-4	1.1664310475950524E-4
15	1.1664310475950524E-4	0.0	0.0	3.499293142785157E-4	1.1664310475950524E-4
16	1.1664310475950524E-4	0.0	0.0	0.0011664310475950526	1.1664310475950524E-4
17	1.1664310475950524E-4	0.0	0.0	0.001866289676152084	1.1664310475950524E-4
18	1.1664310475950524E-4	0.0	0.0	0.002332862095190105	1.1664310475950524E-4
19	1.1664310475950524E-4	0.0	0.0	0.003149363828506642	1.1664310475950524E-4
20	1.1664310475950524E-4	0.0	0.0	0.004199151771342189	1.1664310475950524E-4
21	2.332862095190105E-4	0.0	0.0	0.005948798342734768	1.1664310475950524E-4
22	2.332862095190105E-4	0.0	0.0	0.008048374228405862	2.332862095190105E-4
23	2.332862095190105E-4	0.0	0.0	0.009214805276000914	2.332862095190105E-4
24	2.332862095190105E-4	0.0	0.0	0.01201423979022904	2.332862095190105E-4
25	2.332862095190105E-4	0.0	0.0	0.013880529466381124	2.332862095190105E-4
26	2.332862095190105E-4	0.0	0.0	0.017729751923444798	2.332862095190105E-4
27	2.332862095190105E-4	0.0	0.0	0.04350787807529546	2.332862095190105E-4
28	3.499293142785157E-4	0.0	0.0	0.16773278464416855	2.332862095190105E-4
29	3.499293142785157E-4	0.0	0.0	0.3655594903162894	2.332862095190105E-4
30	3.499293142785157E-4	0.0	0.0	0.6024616360828446	2.332862095190105E-4
31	3.499293142785157E-4	0.0	0.0	1.2871566610211405	2.332862095190105E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1585	0.0	29.296532	1
ATACGGC	125	0.0	23.68	29
GACGTAT	55	1.9019277E-5	23.545454	1
TCTAGAC	40	0.0019307887	23.125002	3
ACGTATA	60	3.724864E-5	21.583334	2
TTTTACG	55	5.141629E-4	20.181818	4
GTTAGGT	65	6.8995134E-5	19.923077	20
ATTTCGG	65	6.8995134E-5	19.923077	29
ATACACA	205	0.0	19.853657	37
CTTATAC	1215	0.0	19.641975	37
GACCGTT	105	2.2573658E-8	19.380953	7
GTACTGG	580	0.0	18.818966	1
GTATCAA	2455	0.0	18.763748	2
TATGCGG	60	9.234204E-4	18.5	5
TACTGCG	50	0.0070334612	18.499998	7
TACTGGT	600	0.0	18.191668	2
TATACAC	410	0.0	18.04878	37
GCGGTAA	695	0.0	17.834532	23
GTGTATA	105	4.7937465E-7	17.61905	10
TTTCGGT	75	2.0664943E-4	17.266666	30
>>END_MODULE