FastQCFastQC Report
Fri 10 Feb 2017
ERR1631752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607622
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65040.40457271672072165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62930.39144774082464656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51030.3174253649178725No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25320.15749971075290087No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24440.15202578715643353No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA22020.13697249726614838No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22020.13697249726614838No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20680.12863720451698224No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20060.12478057652856207No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19760.12291446621158456No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18510.11513900655751165No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17240.10723913954897359No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16650.10356912259225116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.028.7937741
ATACGGC1600.019.6562529
CAGTCGG4900.019.25510410
GCAGTCG4900.018.8775529
GCGGTAA9300.018.69892723
CTCTATG7450.018.624161
GTACTAT609.2390017E-418.51
CTACACT902.1533033E-618.54
GTATCAA40600.017.9987682
CTTATAC18400.017.99728237
ATTTCGG1351.1532393E-917.81481429
AGTCGGT5100.017.7745111
CGCGGTA10100.017.76732622
AATACTG2200.017.659095
AACTCCG10300.017.4223295
TACCGCT852.72421E-517.41176435
AGCCGCG10300.017.24271819
CGTGCCA10800.016.78703710
CCGCGGT10950.016.72602721
GGTAATA10400.016.72115325
Produced by FastQC (version 0.11.5)