##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631752.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1607622
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6150270399385	34.0	31.0	34.0	31.0	34.0
2	32.780869507881825	34.0	31.0	34.0	31.0	34.0
3	32.86723122724123	34.0	31.0	34.0	31.0	34.0
4	36.298208160873635	37.0	37.0	37.0	35.0	37.0
5	36.218909669064	37.0	35.0	37.0	35.0	37.0
6	36.26855877812073	37.0	37.0	37.0	35.0	37.0
7	36.25482234007745	37.0	37.0	37.0	35.0	37.0
8	36.236657000215224	37.0	37.0	37.0	35.0	37.0
9	38.0612930153979	39.0	38.0	39.0	35.0	39.0
10	38.00046279535861	39.0	38.0	39.0	35.0	39.0
11	38.07339225265641	39.0	38.0	39.0	37.0	39.0
12	38.01157237211235	39.0	38.0	39.0	35.0	39.0
13	38.06773420617533	39.0	38.0	39.0	36.0	39.0
14	39.446937153136744	40.0	39.0	41.0	37.0	41.0
15	39.4530250270275	40.0	39.0	41.0	37.0	41.0
16	39.403171889909444	40.0	39.0	41.0	37.0	41.0
17	39.38406478637391	40.0	39.0	41.0	37.0	41.0
18	39.41125090350841	40.0	39.0	41.0	37.0	41.0
19	39.44310665069276	41.0	39.0	41.0	37.0	41.0
20	39.43003641403265	41.0	39.0	41.0	37.0	41.0
21	39.400872841998925	40.0	39.0	41.0	37.0	41.0
22	39.37333527408806	40.0	39.0	41.0	36.0	41.0
23	39.307900115823244	40.0	39.0	41.0	36.0	41.0
24	39.321959390951356	40.0	39.0	41.0	36.0	41.0
25	39.267784964375956	40.0	39.0	41.0	36.0	41.0
26	39.20419352310431	40.0	39.0	41.0	36.0	41.0
27	39.099329319952076	40.0	39.0	41.0	35.0	41.0
28	39.08139973202656	40.0	39.0	41.0	35.0	41.0
29	39.0460916807558	40.0	39.0	41.0	35.0	41.0
30	38.98957777387968	40.0	38.0	41.0	35.0	41.0
31	38.885330009168825	40.0	38.0	41.0	35.0	41.0
32	38.8188790648548	40.0	38.0	41.0	35.0	41.0
33	38.75256061437328	40.0	38.0	41.0	35.0	41.0
34	38.71065835127909	40.0	38.0	41.0	35.0	41.0
35	38.63349904392948	40.0	38.0	41.0	35.0	41.0
36	38.57830447704747	40.0	38.0	41.0	35.0	41.0
37	38.52029768191776	40.0	38.0	41.0	35.0	41.0
38	38.47304465850803	40.0	38.0	41.0	35.0	41.0
39	38.39665107842515	40.0	38.0	41.0	35.0	41.0
40	38.05584770549296	40.0	38.0	41.0	34.0	41.0
41	38.10807577900775	40.0	37.0	41.0	34.0	41.0
42	38.07662870998282	40.0	37.0	41.0	34.0	41.0
43	37.60748671018436	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	2.0
17	5.0
18	15.0
19	24.0
20	47.0
21	127.0
22	243.0
23	450.0
24	816.0
25	1338.0
26	2163.0
27	3543.0
28	5341.0
29	7845.0
30	11420.0
31	15467.0
32	20595.0
33	28082.0
34	40111.0
35	61165.0
36	93625.0
37	174009.0
38	377694.0
39	763490.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.30689801458303	17.621617519541285	13.45844980971895	26.61303465615673
2	19.460793644277075	20.505131181334914	34.59780968411729	25.43626549027072
3	20.48665668919684	21.380710142060757	30.286908240867565	27.845724927874837
4	15.118354936670436	16.687131676476184	34.714814800991775	33.4796985858616
5	16.071315271873612	33.9031190167838	33.766706352612744	16.25885935872985
6	32.36457326411308	34.79711026597048	16.299913785703353	16.53840268421308
7	28.683857274906664	29.501213593742808	21.158394199631505	20.656534931719023
8	26.47911013907498	32.38391860773241	20.55918617685003	20.577785076342572
9	27.50484877664028	14.161911195542235	19.245257902666175	39.08798212515131
10	18.569601560565854	25.117720459162662	30.976995836085848	25.335682144185633
11	36.54391393001589	21.198639978800987	20.456736720447964	21.80070937073516
12	21.90309662345999	25.3501133973036	28.10685596489722	24.63993401433919
13	33.08713117884677	19.01858770283064	22.87509128389634	25.019189834426253
14	23.16937688088369	21.46505832838814	25.144716855081604	30.22084793564656
15	27.773568662285044	25.88257687441451	21.90042186533899	24.44343259796146
16	24.059946927822583	25.542447167306744	24.237227407935446	26.160378496935227
17	24.794634559616625	25.269684042641867	24.22192530333623	25.713756094405277
18	24.909649158819672	23.028547755629123	25.868394436005477	26.193408649545724
19	26.172694825027275	23.90792114066615	26.155464406433847	23.76391962787272
20	27.26580004503546	22.75640666773657	25.603033548931283	24.37475973829669
21	25.548978553416163	24.16046807023044	24.736411917726926	25.554141458626468
22	25.8279620458043	24.448906521557927	24.52268008275577	25.200451349881998
23	25.586487370787413	23.87949406017086	25.296182809142948	25.23783575989878
24	25.740441471938052	23.673973110594407	25.474334140737064	25.111251276730474
25	25.565338120528335	23.823448546984302	25.816267754484574	24.79494557800279
26	25.492373207134513	24.362070188141242	25.166612549467477	24.978944055256772
27	25.14328617050526	23.777915455250053	26.01183611570382	25.066962258540876
28	24.452016705419556	24.523924156300424	25.11044262892645	25.913616509353567
29	25.071689738010548	24.981432202346074	25.101671910436657	24.845206149206717
30	24.856527218463047	24.32008270600925	26.00561574798056	24.81777432754715
31	25.62835044556494	24.033945790739367	25.357888856957665	24.979814906738028
32	23.658671005995192	24.15300362896253	25.639733718498505	26.54859164654378
33	23.97628298194476	23.803543370269878	26.945637718319354	25.27453592946601
34	24.259309713353012	23.66924563112473	26.327270962950244	25.74417369257201
35	24.1474052980116	24.63141211055833	26.581497391799815	24.63968519963026
36	24.314670986090015	23.812687310823065	26.81183760859207	25.06080409449485
37	24.659776987376386	23.33869529031078	26.51593471599667	25.485593006316158
38	23.93392227774937	23.080176807732165	27.454401594404654	25.53149932011381
39	23.63646429322316	22.304248137932923	28.29296936717711	25.76631820166681
40	23.405502039658575	22.965784245301446	28.70973400463542	24.91897971040456
41	22.063022277624963	22.659928764348834	29.459972555737608	25.817076402288595
42	21.22128211731365	22.92858644631636	29.76421074108217	26.08592069528782
43	20.540462870003022	23.607975009050637	29.46227409179521	26.389288029151132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	100.5
2	87.0
3	258.5
4	430.0
5	430.0
6	520.0
7	610.0
8	682.0
9	754.0
10	1210.0
11	1666.0
12	1666.0
13	3163.5
14	4661.0
15	8673.5
16	12686.0
17	12483.5
18	12281.0
19	12281.0
20	13803.5
21	15326.0
22	13585.5
23	11845.0
24	12931.5
25	14018.0
26	14018.0
27	15329.5
28	16641.0
29	21331.5
30	26022.0
31	28421.0
32	30820.0
33	30820.0
34	34487.0
35	38154.0
36	41549.5
37	44945.0
38	51396.5
39	57848.0
40	57848.0
41	66649.0
42	75450.0
43	80034.0
44	84618.0
45	102516.5
46	120415.0
47	120415.0
48	129464.5
49	138514.0
50	151319.0
51	164124.0
52	163983.0
53	163842.0
54	163842.0
55	147268.0
56	130694.0
57	120782.5
58	110871.0
59	101346.0
60	91821.0
61	91821.0
62	82846.5
63	73872.0
64	61475.0
65	49078.0
66	42645.5
67	36213.0
68	36213.0
69	31294.0
70	26375.0
71	22694.0
72	19013.0
73	15616.0
74	12219.0
75	12219.0
76	10021.0
77	7823.0
78	6444.5
79	5066.0
80	4160.5
81	3255.0
82	3255.0
83	2707.5
84	2160.0
85	1874.0
86	1588.0
87	1259.5
88	931.0
89	931.0
90	749.5
91	568.0
92	370.0
93	172.0
94	99.5
95	27.0
96	27.0
97	16.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1607622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.9058778206499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0875669373965	40.3801289664254
2	6.7703900727061574	6.35142179096992
3	2.263636335651857	3.185335481714089
4	1.1501084317255632	2.1578738231607413
5	0.6958559920144075	1.6319868071097465
6	0.46847761824711726	1.318461235392501
7	0.33635487985638096	1.1043914629245948
8	0.23504330369732146	0.8819929988630775
9	0.20038277819138606	0.8459217100086789
>10	1.4128843534077984	13.24312780783913
>50	0.20740693306438648	6.841662743004405
>100	0.15414265149379291	13.734665846928106
>500	0.012411453134602637	3.8435746671543396
>1k	0.004937889956777393	3.3589337050515886
>5k	4.003694559549238E-4	1.1205209534537897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6504	0.40457271672072165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6293	0.39144774082464656	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5103	0.3174253649178725	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2532	0.15749971075290087	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2444	0.15202578715643353	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2202	0.13697249726614838	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2202	0.13697249726614838	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2068	0.12863720451698224	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2006	0.12478057652856207	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1976	0.12291446621158456	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1851	0.11513900655751165	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1724	0.10723913954897359	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1665	0.10356912259225116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.220367723258329E-5	0.0
3	0.0	0.0	0.0	6.220367723258329E-5	0.0
4	0.0	0.0	0.0	6.220367723258329E-5	0.0
5	0.0	0.0	6.220367723258329E-5	6.220367723258329E-5	0.0
6	0.0	0.0	6.220367723258329E-5	6.220367723258329E-5	0.0
7	0.0	0.0	6.220367723258329E-5	1.2440735446516657E-4	0.0
8	1.2440735446516657E-4	0.0	6.220367723258329E-5	1.2440735446516657E-4	0.0
9	1.2440735446516657E-4	0.0	6.220367723258329E-5	1.8661103169774983E-4	0.0
10	1.8661103169774983E-4	0.0	6.220367723258329E-5	2.4881470893033315E-4	0.0
11	1.8661103169774983E-4	0.0	6.220367723258329E-5	2.4881470893033315E-4	0.0
12	1.8661103169774983E-4	0.0	6.220367723258329E-5	4.35425740628083E-4	0.0
13	1.8661103169774983E-4	0.0	6.220367723258329E-5	4.35425740628083E-4	0.0
14	1.8661103169774983E-4	0.0	6.220367723258329E-5	7.464441267909993E-4	0.0
15	1.8661103169774983E-4	0.0	6.220367723258329E-5	8.70851481256166E-4	0.0
16	1.8661103169774983E-4	0.0	6.220367723258329E-5	0.0013062772218842488	0.0
17	3.7322206339549966E-4	0.0	6.220367723258329E-5	0.0019283139942100817	0.0
18	4.35425740628083E-4	0.0	6.220367723258329E-5	0.0023015360576055816	0.0
19	4.35425740628083E-4	0.0	6.220367723258329E-5	0.003110183861629164	0.0
20	4.976294178606663E-4	0.0	6.220367723258329E-5	0.0036700169567224134	0.0
21	4.976294178606663E-4	0.0	6.220367723258329E-5	0.005971553014327995	6.220367723258329E-5
22	4.976294178606663E-4	0.0	6.220367723258329E-5	0.009703773648282992	6.220367723258329E-5
23	4.976294178606663E-4	0.0	6.220367723258329E-5	0.011569883965260491	6.220367723258329E-5
24	4.976294178606663E-4	0.0	6.220367723258329E-5	0.013933623700098655	6.220367723258329E-5
25	4.976294178606663E-4	0.0	6.220367723258329E-5	0.015799734017076152	6.220367723258329E-5
26	4.976294178606663E-4	0.0	6.220367723258329E-5	0.01890991787870532	6.220367723258329E-5
27	4.976294178606663E-4	0.0	6.220367723258329E-5	0.040681204910109466	6.220367723258329E-5
28	5.598330950932495E-4	0.0	6.220367723258329E-5	0.13715910829784614	6.220367723258329E-5
29	5.598330950932495E-4	0.0	6.220367723258329E-5	0.2812850284457416	6.220367723258329E-5
30	5.598330950932495E-4	0.0	6.220367723258329E-5	0.4622355255153264	6.220367723258329E-5
31	5.598330950932495E-4	0.0	6.220367723258329E-5	1.0070153307182907	6.220367723258329E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2570	0.0	28.793774	1
ATACGGC	160	0.0	19.65625	29
CAGTCGG	490	0.0	19.255104	10
GCAGTCG	490	0.0	18.877552	9
GCGGTAA	930	0.0	18.698927	23
CTCTATG	745	0.0	18.62416	1
GTACTAT	60	9.2390017E-4	18.5	1
CTACACT	90	2.1533033E-6	18.5	4
GTATCAA	4060	0.0	17.998768	2
CTTATAC	1840	0.0	17.997282	37
ATTTCGG	135	1.1532393E-9	17.814814	29
AGTCGGT	510	0.0	17.77451	11
CGCGGTA	1010	0.0	17.767326	22
AATACTG	220	0.0	17.65909	5
AACTCCG	1030	0.0	17.422329	5
TACCGCT	85	2.72421E-5	17.411764	35
AGCCGCG	1030	0.0	17.242718	19
CGTGCCA	1080	0.0	16.787037	10
CCGCGGT	1095	0.0	16.726027	21
GGTAATA	1040	0.0	16.721153	25
>>END_MODULE