FastQCFastQC Report
Fri 10 Feb 2017
ERR1631751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055165
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.30251192941388316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30320.28734842418010453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24630.23342320869247937No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15810.14983438609127483No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15130.14338989636691893No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14510.1375140380888297No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12500.11846488463889535No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12460.11808579700805087No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12240.11600081503840631No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11710.11097790392971717No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11690.11078836011429492No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11520.10917723768320595No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10570.10017390645064991No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACTC250.005496142429.5999983
GGTATCA14550.028.6082481
CGTCTAT451.3231114E-424.66666827
TAGTGGT400.001931082623.1250024
TTTTACG903.8271537E-922.6111134
GTAAGAC603.725914E-521.5833323
CTTATAC10250.020.57561137
TCTAGGT555.14271E-420.1818182
GTCTATG656.901445E-519.92307728
CTCTATG4700.018.8936181
GTATCAA22300.018.582962
TATACAC3600.018.537
CGAATTA500.007034510418.49999815
TGTTACG500.007034510418.49999816
CCGAATT500.007034510418.49999814
TCTATGG4900.017.7448982
TAGAACT1800.017.4722234
TGCGGGT3950.017.32911321
CGTAATG650.001579817917.0769232
GCGTAAT650.001579817917.0769231
Produced by FastQC (version 0.11.5)