##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631751.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055165
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36300957670127	33.0	31.0	34.0	31.0	34.0
2	32.542261162946076	34.0	31.0	34.0	31.0	34.0
3	32.630858680869814	34.0	31.0	34.0	31.0	34.0
4	36.154430823615265	37.0	35.0	37.0	35.0	37.0
5	36.01921784744566	37.0	35.0	37.0	35.0	37.0
6	36.061986513957535	37.0	35.0	37.0	35.0	37.0
7	36.046003231722054	37.0	35.0	37.0	35.0	37.0
8	36.02415262068018	37.0	35.0	37.0	35.0	37.0
9	37.81162851307615	39.0	38.0	39.0	35.0	39.0
10	37.71492894476219	39.0	37.0	39.0	35.0	39.0
11	37.79909871915767	39.0	38.0	39.0	35.0	39.0
12	37.73380750877825	39.0	37.0	39.0	35.0	39.0
13	37.79116251960594	39.0	38.0	39.0	35.0	39.0
14	39.10391455364801	40.0	38.0	41.0	36.0	41.0
15	39.09710424435988	40.0	38.0	41.0	36.0	41.0
16	39.04180199305322	40.0	38.0	41.0	36.0	41.0
17	39.017879668108776	40.0	38.0	41.0	36.0	41.0
18	39.03680561807869	40.0	38.0	41.0	36.0	41.0
19	39.069879118431714	40.0	39.0	41.0	36.0	41.0
20	39.05784972018594	40.0	39.0	41.0	36.0	41.0
21	39.024582885141186	40.0	38.0	41.0	36.0	41.0
22	38.99623945070202	40.0	38.0	41.0	36.0	41.0
23	38.93451545492885	40.0	38.0	41.0	35.0	41.0
24	38.945793311946474	40.0	38.0	41.0	35.0	41.0
25	38.8828894059223	40.0	38.0	41.0	35.0	41.0
26	38.80427326531869	40.0	38.0	41.0	35.0	41.0
27	38.69997772860169	40.0	38.0	41.0	35.0	41.0
28	38.68076272431326	40.0	38.0	41.0	35.0	41.0
29	38.648147919993555	40.0	38.0	41.0	35.0	41.0
30	38.58833547359892	40.0	38.0	41.0	35.0	41.0
31	38.48919552866139	40.0	38.0	41.0	35.0	41.0
32	38.429097818824545	40.0	38.0	41.0	34.0	41.0
33	38.364983675538895	40.0	38.0	41.0	34.0	41.0
34	38.325242971478396	40.0	38.0	41.0	34.0	41.0
35	38.24186359479323	40.0	38.0	41.0	34.0	41.0
36	38.18727876682794	40.0	38.0	41.0	34.0	41.0
37	38.13927016153872	40.0	38.0	41.0	34.0	41.0
38	38.0913648576289	40.0	37.0	41.0	34.0	41.0
39	38.02636459700616	40.0	37.0	41.0	33.0	41.0
40	37.69811451289609	40.0	37.0	41.0	33.0	41.0
41	37.764139257841194	40.0	37.0	41.0	33.0	41.0
42	37.72958352485156	40.0	37.0	41.0	33.0	41.0
43	37.22396781546014	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	1.0
18	9.0
19	34.0
20	58.0
21	114.0
22	230.0
23	448.0
24	717.0
25	1222.0
26	2009.0
27	3077.0
28	4636.0
29	6634.0
30	9305.0
31	12557.0
32	16824.0
33	22888.0
34	32494.0
35	48121.0
36	75562.0
37	138034.0
38	302750.0
39	377437.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.23386863665872	17.626721887098228	13.432685883250487	26.706723592992564
2	19.588689920533756	20.304217823752683	34.43650992972663	25.670582325986928
3	20.586543336824096	21.106651566342705	30.133201916287973	28.17360318054522
4	15.431994048324196	16.64564309847275	34.03752019826283	33.88484265494022
5	16.1379499888643	33.869394833983314	33.78770144953633	16.204953727616058
6	33.267687991925435	34.284874877388845	16.045073519307408	16.402363611378316
7	29.508655044471716	29.000108987693867	20.88715982808376	20.604076139750653
8	26.496045642150754	32.53424819814911	20.470258206062557	20.499447953637585
9	27.58848142233679	14.144896769699525	18.9863196751219	39.28030213284178
10	18.56704875540792	25.17644159918117	31.143565224396184	25.11294442101472
11	36.73055872778191	20.932934659508227	20.420787270237355	21.915719342472503
12	22.092279406538314	24.872887178782467	27.93060800917392	25.104225405505296
13	32.69924608947416	18.81990020518118	23.09714594399928	25.38370776134538
14	23.38496822771794	21.046471404946146	24.678415224159256	30.89014514317666
15	27.686191259186955	25.78070728274725	21.748541697270095	24.784559760795705
16	24.521188629266515	25.42047926153729	23.78395795918174	26.274374150014452
17	25.20079797946293	25.198239137954726	23.890386811541322	25.710576071041025
18	25.036084403861008	23.232669772026178	25.374799202020537	26.356446622092278
19	26.460032317220527	23.797794657707563	25.582823539446437	24.15934948562547
20	27.39154539811309	22.70062028213597	25.29083129178849	24.61700302796245
21	25.530698990205323	24.096326166997578	24.365857472527992	26.007117370269107
22	26.060853041941307	24.30188643482299	23.91057322788379	25.726687295351912
23	25.66660190586306	23.94525974610606	24.82739666308113	25.560741684949743
24	26.15932105405316	23.587969654035152	24.89212587604782	25.360583415863868
25	26.09459184108647	23.394824506119896	25.259272246520688	25.251311406272954
26	25.936322755208902	24.198016423971605	24.678225680343832	25.18743514047566
27	25.521127027526504	23.515469144636146	25.44549904517303	25.517904782664324
28	24.716703074874545	24.334298427260194	24.746366681988125	26.202631815877137
29	25.45004809674316	24.701918657271612	24.718029881582503	25.130003364402725
30	25.062241450389276	24.270232617647476	25.391099970146847	25.2764259618164
31	26.034980311136174	23.78395795918174	24.977515364895538	25.20354636478655
32	24.140774191714094	23.995678401008373	25.102993370705057	26.760554036572483
33	24.41741339032284	23.46912568176541	26.40004169963939	25.713419228272354
34	24.78759246184246	23.36724588097596	25.825344851279187	26.019816805902394
35	24.649604564215075	24.375334663299107	26.031852838181706	24.943207934304112
36	24.67130733108092	23.657342690479688	26.328773225040635	25.34257675339876
37	25.08877758454839	23.0606587595305	26.047111115323197	25.803452540597917
38	24.343112214677326	22.78383001710633	27.00250671695896	25.870551051257383
39	23.961370970416947	22.075694322688868	27.86123497272938	26.1016997341648
40	23.627584311458396	22.837660460686244	28.16990707614449	25.364848151710873
41	22.455540128795022	22.478001070922556	29.07241995327745	25.99403884700497
42	21.815450664114145	22.652665696834145	29.078485355370958	26.45339828368075
43	21.19621101912971	23.2524771007378	29.14131913018343	26.409992749949062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	55.5
2	61.0
3	122.5
4	184.0
5	184.0
6	232.5
7	281.0
8	310.0
9	339.0
10	526.0
11	713.0
12	713.0
13	1436.5
14	2160.0
15	4127.0
16	6094.0
17	5952.5
18	5811.0
19	5811.0
20	6600.0
21	7389.0
22	6739.0
23	6089.0
24	6632.0
25	7175.0
26	7175.0
27	7949.0
28	8723.0
29	11598.5
30	14474.0
31	16136.0
32	17798.0
33	17798.0
34	20766.0
35	23734.0
36	26013.0
37	28292.0
38	33857.5
39	39423.0
40	39423.0
41	44883.0
42	50343.0
43	53740.0
44	57137.0
45	69416.5
46	81696.0
47	81696.0
48	88013.0
49	94330.0
50	103419.5
51	112509.0
52	112452.0
53	112395.0
54	112395.0
55	99644.0
56	86893.0
57	80061.0
58	73229.0
59	65696.0
60	58163.0
61	58163.0
62	52981.0
63	47799.0
64	40821.0
65	33843.0
66	29410.0
67	24977.0
68	24977.0
69	21434.5
70	17892.0
71	15506.5
72	13121.0
73	10622.0
74	8123.0
75	8123.0
76	6687.0
77	5251.0
78	4406.5
79	3562.0
80	2852.5
81	2143.0
82	2143.0
83	1753.5
84	1364.0
85	1102.5
86	841.0
87	658.0
88	475.0
89	475.0
90	347.5
91	220.0
92	137.5
93	55.0
94	33.5
95	12.0
96	12.0
97	7.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1055165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.146905185764155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74255832596641	42.13981384431124
2	7.106811483859995	6.985555803407341
3	2.2386333505705522	3.3006570307854135
4	1.126154541529703	2.2138804190831207
5	0.6870320486345523	1.6882749476911822
6	0.48646847479286726	1.4345052003905008
7	0.338577159124287	1.16480136762827
8	0.2581357412341203	1.014925823959221
9	0.1943745247709306	0.8597615705500404
>10	1.4723894277284717	14.561911227315147
>50	0.1937102331751969	6.682681856867455
>100	0.1452759257208599	13.399331658633276
>500	0.006973244394399661	2.314076907640179
>1k	0.0029055184976665258	2.2398223417376224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3192	0.30251192941388316	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3032	0.28734842418010453	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2463	0.23342320869247937	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1581	0.14983438609127483	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1513	0.14338989636691893	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1451	0.1375140380888297	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1250	0.11846488463889535	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1246	0.11808579700805087	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1224	0.11600081503840631	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1171	0.11097790392971717	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1169	0.11078836011429492	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1152	0.10917723768320595	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1057	0.10017390645064991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.477190771111628E-5	0.0
4	0.0	0.0	0.0	9.477190771111628E-5	0.0
5	0.0	0.0	0.0	9.477190771111628E-5	0.0
6	0.0	0.0	0.0	9.477190771111628E-5	0.0
7	0.0	0.0	0.0	9.477190771111628E-5	0.0
8	9.477190771111628E-5	0.0	0.0	9.477190771111628E-5	0.0
9	9.477190771111628E-5	0.0	0.0	9.477190771111628E-5	0.0
10	1.8954381542223256E-4	0.0	0.0	9.477190771111628E-5	0.0
11	1.8954381542223256E-4	0.0	0.0	9.477190771111628E-5	0.0
12	1.8954381542223256E-4	0.0	0.0	1.8954381542223256E-4	0.0
13	1.8954381542223256E-4	0.0	0.0	1.8954381542223256E-4	0.0
14	1.8954381542223256E-4	0.0	0.0	2.843157231333488E-4	0.0
15	2.843157231333488E-4	0.0	0.0	2.843157231333488E-4	0.0
16	2.843157231333488E-4	0.0	0.0	6.634033539778139E-4	0.0
17	2.843157231333488E-4	0.0	0.0	0.0011372628925333953	0.0
18	2.843157231333488E-4	0.0	0.0	0.0011372628925333953	0.0
19	2.843157231333488E-4	0.0	0.0	0.0016111224310889766	9.477190771111628E-5
20	2.843157231333488E-4	0.0	0.0	0.0019902100619334415	9.477190771111628E-5
21	3.790876308444651E-4	0.0	0.0	0.003411788677600186	9.477190771111628E-5
22	3.790876308444651E-4	0.0	0.0	0.005496770647244744	9.477190771111628E-5
23	3.790876308444651E-4	0.0	0.0	0.006918349262911488	9.477190771111628E-5
24	3.790876308444651E-4	0.0	0.0	0.008908559324844929	9.477190771111628E-5
25	3.790876308444651E-4	0.0	0.0	0.010140594125089442	9.477190771111628E-5
26	3.790876308444651E-4	0.0	0.0	0.01269943563328958	9.477190771111628E-5
27	3.790876308444651E-4	0.0	0.0	0.03051655428297944	9.477190771111628E-5
28	3.790876308444651E-4	0.0	0.0	0.1060497647287391	9.477190771111628E-5
29	3.790876308444651E-4	0.0	0.0	0.22821075376836797	9.477190771111628E-5
30	3.790876308444651E-4	0.0	0.0	0.38714324299991	9.477190771111628E-5
31	4.7385953855558135E-4	0.0	0.0	0.895025896423782	9.477190771111628E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACTC	25	0.0054961424	29.599998	3
GGTATCA	1455	0.0	28.608248	1
CGTCTAT	45	1.3231114E-4	24.666668	27
TAGTGGT	40	0.0019310826	23.125002	4
TTTTACG	90	3.8271537E-9	22.611113	4
GTAAGAC	60	3.725914E-5	21.583332	3
CTTATAC	1025	0.0	20.575611	37
TCTAGGT	55	5.14271E-4	20.181818	2
GTCTATG	65	6.901445E-5	19.923077	28
CTCTATG	470	0.0	18.893618	1
GTATCAA	2230	0.0	18.58296	2
TATACAC	360	0.0	18.5	37
CGAATTA	50	0.0070345104	18.499998	15
TGTTACG	50	0.0070345104	18.499998	16
CCGAATT	50	0.0070345104	18.499998	14
TCTATGG	490	0.0	17.744898	2
TAGAACT	180	0.0	17.472223	4
TGCGGGT	395	0.0	17.329113	21
CGTAATG	65	0.0015798179	17.076923	2
GCGTAAT	65	0.0015798179	17.076923	1
>>END_MODULE