FastQCFastQC Report
Fri 10 Feb 2017
ERR1631750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797464
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62050.778091550214179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58900.7385913345304616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45650.5724396336386345No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA35000.43889128537463756No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28140.35286859344120863No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27820.3488558731177834No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA26110.32741289888947966No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA24760.31048423502502936No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA24550.3078508873127815No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23520.2949349437717565No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC21950.2752475346849513No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT21760.27286498199291753No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG21190.26571732391681635No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA21060.2640871562854248No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20870.26170460359339104No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20790.2607014235125348No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC20660.2590712558811432No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG19780.24803627499172376No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA19780.24803627499172376No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC19240.2412648094459436No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19060.23900765426401693No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA19030.23863146173369582No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC18880.23675049908209023No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA18510.23211079120812977No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17800.22320756799052996No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA17750.22258058043999476No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17490.21932024517721174No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16470.20652969914629377No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16460.2064043016361867No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA15540.1948677307063391No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15110.1894756377717364No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14250.17869145190253102No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA13790.17292316643760722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.17104220378600163No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13560.17003902370514531No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT13530.1696628311748242No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC13340.16728027848279045No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT13140.16477232828064967No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13070.16389454570990036No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA12940.16226437807850888No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12880.16151199301786665No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC12640.1585024527752977No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12060.15122939718908943No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11940.14972462706780496No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT11920.14947383204759085No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11760.14746747188587825No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11690.14658968931512897No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11540.14470872666352338No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11460.14370554658266704No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT11410.14307855903213185No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11120.1394420312390277No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11030.13831345364806436No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10960.13743567107731508No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG10920.13693408103688692No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10790.13530391340549544No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA10380.1301626154911068No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT10350.12978642296078569No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10270.12878324287992937No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10270.12878324287992937No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC10250.1285324478597153No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10190.12778006279907306No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG10150.1272784727586449No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC10110.12677688271821674No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10070.1262752926777886No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9970.1250213175767182No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9850.12351654745543374No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9730.12201177733414925No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA9730.12201177733414925No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9670.121259392273507No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC9660.12113399476339998No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9360.1173720694601888No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA9270.11624349186922543No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9220.11561650431869024No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT9010.11298315660644243No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9010.11298315660644243No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8950.11223077154580018No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA8920.11185457901547906No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8890.11147838648515795No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC8700.1090958337931242No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC8650.108468846242589No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC8610.10796725620216086No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT8580.10759106367183974No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8570.10746566616173268No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8540.10708947363141158No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8410.10545930600002007No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC8400.10533390848991302No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8400.10533390848991302No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA8390.10520851097980599No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA8160.10232436824734409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTTA200.001841597837.014
GGTATCA21050.031.111641
GTCTATA250.00549534729.5999982
CCCGTTA250.00549534729.5999988
ACATGCC250.00549534729.59999833
TATACCC250.00549534729.5999985
CCTGCAA250.00549534729.59999810
TAAGGTA556.2577055E-726.909095
AACCCTG1400.023.78571536
GTACTTG551.9017118E-523.5454546
ACTCCCA551.9017118E-523.5454545
TGTACTT551.9017118E-523.5454545
ATACACA1350.023.29629537
GATGGCA400.001930671223.12500225
AAGGTAG652.6811358E-622.769236
ATGCCCC652.6811358E-622.7692337
GGTGTTA502.701097E-422.19999912
TCCTGTA502.701097E-422.1999992
TCAATGC502.701097E-422.19999932
TTGGTGT502.701097E-422.19999910
Produced by FastQC (version 0.11.5)