FastQCFastQC Report
Fri 10 Feb 2017
ERR1631749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191069
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3680.19260057884847884No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3570.18684349632855146No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2920.15282437234716253No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2900.1517776300708121No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2870.15020751665628648No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2670.1397400938927822No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2520.13188952682015398No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2520.13188952682015398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2520.13188952682015398No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2440.12770255771475228No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2440.12770255771475228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.12665581543840182No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2340.12246884633300012No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2300.12037536178029927No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2280.11932861950394882No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2240.11723513495124797No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2180.11409490812219669No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2130.11147805243132063No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2120.1109546812931454No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2000.10467422763504285No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1960.10258074308234198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCGC303.5883536E-430.83333218
GGTATCA2850.030.5087741
ATCGGGT250.005485022429.59999812
GCGCTAT250.005485022429.5999981
GTGTCCG358.8422414E-426.4285717
TGGCTAG358.8422414E-426.4285725
CTTATAC2500.024.42000237
CAGCATT400.001925330823.12534
TGTACCA400.001925330823.12529
AGCACTG400.001925330823.1255
GTATCAA3950.022.4810122
CGCGGTA1250.022.222
GCGGTAA1301.8189894E-1221.34615323
GTGCAAA450.003814318220.55555511
GTACCAG450.003814318220.55555530
GTCCTAC450.003814318220.5555551
CATCCCA450.003814318220.55555516
TATACAC450.003814318220.55555537
GGCCTGA450.003814318220.5555556
ATACGGC555.1215535E-420.18181829
Produced by FastQC (version 0.11.5)