FastQCFastQC Report
Fri 10 Feb 2017
ERR1631748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881998
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC27820.3154202163723727No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT22450.25453572457080403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18260.2070299479137141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.20510250590137394No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15020.1702951707373486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14050.15929741337281944No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG12800.1451250456350241No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT12670.1436511193902934No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT12160.13786879335327293No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG11630.1318597094324477No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11450.12981888847820516No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG11120.1260773833954272No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10920.12380980455737994No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC10730.12165560466123507No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT10360.11746058381084765No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10100.1145127313213862No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10040.11383245766997203No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10010.11349232084426496No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC9940.11269866825094842No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG9710.11009095258719408No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9690.10986419470338936No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT9480.10748323692343974No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9450.10714310009773266No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG9410.1066895843301232No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG9370.10623606856251375No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT9350.10600931067870903No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9140.10362835289875941No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG8900.1009072582931027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.031.5255091
AGGTATA358.8677544E-426.428578
GACAGGC7600.023.1257
CCAGGAC8800.022.9147763
ACAGGCT7900.022.2468368
TCCAGGA8700.022.1149432
GGACAGG8700.021.0517256
CAGGACA9500.020.6421054
TAGGTTG450.003825113220.5555555
TTAACGA450.003825113220.55555512
GTATCAA15250.020.2590162
CATCAGA8850.019.85875916
TGCATCA8900.019.7471914
TAATACT759.2617665E-619.7333324
ACTGAGC851.2446362E-619.5882348
GCGGTAA5500.019.50909223
ATACACA1900.019.47368437
AGGCCAT9050.019.4198925
AGAAGAG9500.019.0842120
CGTGCCA5550.019.010
Produced by FastQC (version 0.11.5)