##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881998
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.600662359778596	34.0	31.0	34.0	31.0	34.0
2	32.770280658232785	34.0	31.0	34.0	31.0	34.0
3	32.853467921695966	34.0	31.0	34.0	31.0	34.0
4	36.2873090415171	37.0	37.0	37.0	35.0	37.0
5	36.20719774874773	37.0	35.0	37.0	35.0	37.0
6	36.251551590819936	37.0	37.0	37.0	35.0	37.0
7	36.23937469245962	37.0	37.0	37.0	35.0	37.0
8	36.21452883113113	37.0	37.0	37.0	35.0	37.0
9	38.04821212746514	39.0	38.0	39.0	35.0	39.0
10	37.987630357438455	39.0	38.0	39.0	35.0	39.0
11	38.06471329866961	39.0	38.0	39.0	37.0	39.0
12	38.00883788852129	39.0	38.0	39.0	35.0	39.0
13	38.04975861623269	39.0	38.0	39.0	35.0	39.0
14	39.446563370892	40.0	39.0	41.0	37.0	41.0
15	39.44406790038072	41.0	39.0	41.0	37.0	41.0
16	39.40115850602836	40.0	39.0	41.0	37.0	41.0
17	39.36670151179481	40.0	39.0	41.0	36.0	41.0
18	39.354059759772696	40.0	39.0	41.0	36.0	41.0
19	39.371107417477134	40.0	39.0	41.0	36.0	41.0
20	39.35361758189928	40.0	39.0	41.0	36.0	41.0
21	39.335055181531025	40.0	39.0	41.0	36.0	41.0
22	39.308929271948465	40.0	39.0	41.0	36.0	41.0
23	39.239484670033264	40.0	39.0	41.0	36.0	41.0
24	39.24917403440824	40.0	39.0	41.0	36.0	41.0
25	39.19230088957118	40.0	39.0	41.0	36.0	41.0
26	39.11640842722999	40.0	39.0	41.0	35.0	41.0
27	39.02077555731419	40.0	39.0	41.0	35.0	41.0
28	39.00117007068043	40.0	39.0	41.0	35.0	41.0
29	38.971384288853265	40.0	38.0	41.0	35.0	41.0
30	38.92276059582902	40.0	38.0	41.0	35.0	41.0
31	38.84654840487167	40.0	38.0	41.0	35.0	41.0
32	38.803417921582586	40.0	38.0	41.0	35.0	41.0
33	38.73793931505514	40.0	38.0	41.0	35.0	41.0
34	38.67860357959995	40.0	38.0	41.0	35.0	41.0
35	38.594346018925215	40.0	38.0	41.0	35.0	41.0
36	38.55216224980102	40.0	38.0	41.0	35.0	41.0
37	38.50342404404545	40.0	38.0	41.0	35.0	41.0
38	38.47569835759265	40.0	38.0	41.0	35.0	41.0
39	38.40417325209354	40.0	38.0	41.0	34.0	41.0
40	38.05149558162263	40.0	37.0	41.0	34.0	41.0
41	38.105832439529344	40.0	37.0	41.0	34.0	41.0
42	38.08752514178037	40.0	37.0	41.0	34.0	41.0
43	37.604563729169456	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	1.0
15	0.0
16	0.0
17	5.0
18	4.0
19	16.0
20	30.0
21	58.0
22	122.0
23	245.0
24	461.0
25	777.0
26	1277.0
27	1996.0
28	2918.0
29	4339.0
30	6162.0
31	8551.0
32	11602.0
33	15655.0
34	23009.0
35	37207.0
36	53768.0
37	94068.0
38	204613.0
39	415111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.795224025451304	15.768403102954881	12.162272476808338	28.274100394785474
2	20.596305207041286	18.379293377082487	33.63839827301195	27.38600314286427
3	21.70707870085873	19.631110274626472	28.30493946698292	30.35687155753188
4	15.906158517366254	15.188356436182396	32.58329383966857	36.32219120678278
5	17.52282884995204	32.80574332368101	32.19077594280259	17.480651883564363
6	35.82275696770287	32.1046079469568	15.23087353939578	16.841761545944546
7	31.457214188694305	27.146320059682676	19.69052084018331	21.70594491143971
8	28.152558169066143	30.910614309783014	19.456960219864445	21.479867301286397
9	28.646890355760448	13.074519443354745	17.470221020909342	40.808369179975465
10	20.13439939773106	23.450166553665657	29.25947677885891	27.155957269744373
11	38.39532515946748	19.822607307499563	19.024759693332637	22.757307839700317
12	24.331914584840327	22.937353599441266	26.2981321953111	26.432599620407306
13	33.802457601944674	17.34663797423577	21.617849473581575	27.233054950237985
14	24.24030439978322	19.593241708031083	23.62941866081329	32.53703523137241
15	29.40550885602915	23.462978374100622	20.462971571364108	26.66854119850612
16	25.701192066195162	23.518307297748976	21.869097208837207	28.91140342721866
17	26.38498046480831	23.849373808103874	22.599824489397935	27.16582123768988
18	26.461397871650504	21.169322379415824	23.856289923559917	28.51298982537375
19	28.095528561289257	22.027714348558614	23.257762489257345	26.618994600894787
20	27.983056650922112	21.43338193510643	23.677377953237986	26.906183460733473
21	28.753693319032468	21.799029022741546	22.342681049163378	27.104596609062604
22	28.64711711364425	22.813883931709597	21.66138698727208	26.877611967374076
23	28.11491636035456	22.446196023120237	22.233270370227597	27.20561724629761
24	28.227274891779803	21.72850732087828	22.7372397669836	27.306978020358326
25	27.557091966194935	21.79766847543872	23.542797149199885	27.10244240916646
26	28.02614064884501	22.365470216485754	23.37125481010161	26.23713432456763
27	28.155619400497507	21.783609486642828	23.308102739461994	26.75266837339767
28	26.434527062419644	22.186898382989533	23.307309086868678	28.07126546772215
29	27.505277789745552	22.439053149780385	23.270007414982803	26.78566164549126
30	26.721829301200227	21.685083186129674	24.423298012013632	27.169789500656467
31	27.186116068290406	22.039845895342168	23.310937213009552	27.463100823357877
32	25.549604420871702	22.09143331390774	23.768194485701784	28.59076777951877
33	26.498926301420184	22.286104957154098	24.263660461815107	26.951308279610615
34	26.460037324347674	22.27896208381425	24.051981977283397	27.20901861455468
35	26.689629681699962	22.964677924439737	24.52295810194581	25.822734291914497
36	26.755389468003326	22.055945705092302	23.91876172054812	27.269903106356253
37	25.665704457379725	22.02465311712725	24.711847419155145	27.597795006337883
38	26.2740958596278	21.077712194358718	25.44813026786909	27.200061678144394
39	24.705044682641002	20.84086358472468	26.602327896435142	27.851763836199172
40	25.047562466128042	21.00594332413452	27.633622751979033	26.31287145775841
41	23.48304644681734	20.533379894285474	28.272172952773133	27.71140070612405
42	22.389733310052858	21.515241531159933	28.248703511799345	27.84632164698786
43	22.065809673037805	20.804922460141633	28.720473289055075	28.408794577765484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	41.0
2	27.0
3	60.0
4	93.0
5	93.0
6	141.5
7	190.0
8	186.5
9	183.0
10	285.0
11	387.0
12	387.0
13	821.0
14	1255.0
15	2249.5
16	3244.0
17	3092.0
18	2940.0
19	2940.0
20	3365.5
21	3791.0
22	3157.5
23	2524.0
24	2617.5
25	2711.0
26	2711.0
27	3166.5
28	3622.0
29	4589.0
30	5556.0
31	6901.5
32	8247.0
33	8247.0
34	10845.5
35	13444.0
36	15217.0
37	16990.0
38	20187.5
39	23385.0
40	23385.0
41	27151.5
42	30918.0
43	36168.5
44	41419.0
45	50749.0
46	60079.0
47	60079.0
48	64572.5
49	69066.0
50	77278.5
51	85491.0
52	87993.0
53	90495.0
54	90495.0
55	86626.5
56	82758.0
57	76743.5
58	70729.0
59	64584.5
60	58440.0
61	58440.0
62	57082.0
63	55724.0
64	44716.0
65	33708.0
66	30378.0
67	27048.0
68	27048.0
69	23644.5
70	20241.0
71	19202.0
72	18163.0
73	20583.0
74	23003.0
75	23003.0
76	19843.0
77	16683.0
78	11207.0
79	5731.0
80	3681.5
81	1632.0
82	1632.0
83	1322.5
84	1013.0
85	776.5
86	540.0
87	412.0
88	284.0
89	284.0
90	207.0
91	130.0
92	87.5
93	45.0
94	28.5
95	12.0
96	12.0
97	6.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	881998.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.035258906119424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.40740406944053	36.755297466279316
2	6.898201092116282	5.937317399714
3	2.2440669530802055	2.897220069854196
4	1.1438672452484793	1.9690649221399164
5	0.7358189643138684	1.5833079818639986
6	0.5281159004904162	1.3636562706026079
7	0.37131531171933063	1.1185755402953483
8	0.30468147097310794	1.0489636789780008
9	0.23255324314123232	0.9007190125236607
>10	1.7169386946549507	14.839553401326134
>50	0.20282571868452368	6.125556437002772
>100	0.19196936798448525	17.382321759747317
>500	0.01747583211994055	5.233316130230101
>1k	0.004766136032711059	2.8451299294426935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2782	0.3154202163723727	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	2245	0.25453572457080403	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1826	0.2070299479137141	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1809	0.20510250590137394	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1502	0.1702951707373486	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1405	0.15929741337281944	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1280	0.1451250456350241	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1267	0.1436511193902934	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1216	0.13786879335327293	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1163	0.1318597094324477	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1145	0.12981888847820516	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1112	0.1260773833954272	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1092	0.12380980455737994	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	1073	0.12165560466123507	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1036	0.11746058381084765	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1010	0.1145127313213862	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1004	0.11383245766997203	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1001	0.11349232084426496	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	994	0.11269866825094842	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	971	0.11009095258719408	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	969	0.10986419470338936	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	948	0.10748323692343974	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	945	0.10714310009773266	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	941	0.1066895843301232	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	937	0.10623606856251375	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	935	0.10600931067870903	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	914	0.10362835289875941	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	890	0.1009072582931027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.267578838047252E-4	0.0	0.0	0.0	0.0
6	2.267578838047252E-4	0.0	0.0	0.0	0.0
7	2.267578838047252E-4	0.0	1.133789419023626E-4	0.0	0.0
8	3.4013682570708777E-4	0.0	1.133789419023626E-4	0.0	0.0
9	3.4013682570708777E-4	0.0	1.133789419023626E-4	0.0	0.0
10	4.535157676094504E-4	0.0	1.133789419023626E-4	0.0	0.0
11	4.535157676094504E-4	0.0	1.133789419023626E-4	0.0	0.0
12	4.535157676094504E-4	0.0	1.133789419023626E-4	0.0	0.0
13	4.535157676094504E-4	0.0	1.133789419023626E-4	0.0	0.0
14	4.535157676094504E-4	0.0	1.133789419023626E-4	0.0	0.0
15	5.66894709511813E-4	0.0	1.133789419023626E-4	0.0	0.0
16	5.66894709511813E-4	0.0	1.133789419023626E-4	6.802736514141755E-4	0.0
17	5.66894709511813E-4	0.0	1.133789419023626E-4	0.001133789419023626	0.0
18	6.802736514141755E-4	0.0	1.133789419023626E-4	0.001360547302828351	0.0
19	7.936525933165381E-4	0.0	1.133789419023626E-4	0.0018140630704378015	0.0
20	7.936525933165381E-4	0.0	1.133789419023626E-4	0.0020408209542425266	0.0
21	7.936525933165381E-4	0.0	1.133789419023626E-4	0.0026077156637543397	0.0
22	7.936525933165381E-4	0.0	1.133789419023626E-4	0.003854884024680328	0.0
23	7.936525933165381E-4	0.0	1.133789419023626E-4	0.004195020850387416	0.0
24	7.936525933165381E-4	0.0	1.133789419023626E-4	0.005328810269411042	0.0
25	7.936525933165381E-4	0.0	1.133789419023626E-4	0.006462599688434668	0.0
26	7.936525933165381E-4	0.0	1.133789419023626E-4	0.009523831119798458	0.0
27	9.070315352189008E-4	0.0	1.133789419023626E-4	0.031405966906954436	0.0
28	9.070315352189008E-4	0.0	1.133789419023626E-4	0.13276674096766658	0.0
29	9.070315352189008E-4	0.0	1.133789419023626E-4	0.27698475506747183	0.0
30	0.0010204104771212633	0.0	1.133789419023626E-4	0.4736972192680709	0.0
31	0.0010204104771212633	0.0	1.133789419023626E-4	1.0123605722461955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	980	0.0	31.525509	1
AGGTATA	35	8.8677544E-4	26.42857	8
GACAGGC	760	0.0	23.125	7
CCAGGAC	880	0.0	22.914776	3
ACAGGCT	790	0.0	22.246836	8
TCCAGGA	870	0.0	22.114943	2
GGACAGG	870	0.0	21.051725	6
CAGGACA	950	0.0	20.642105	4
TAGGTTG	45	0.0038251132	20.555555	5
TTAACGA	45	0.0038251132	20.555555	12
GTATCAA	1525	0.0	20.259016	2
CATCAGA	885	0.0	19.858759	16
TGCATCA	890	0.0	19.74719	14
TAATACT	75	9.2617665E-6	19.733332	4
ACTGAGC	85	1.2446362E-6	19.588234	8
GCGGTAA	550	0.0	19.509092	23
ATACACA	190	0.0	19.473684	37
AGGCCAT	905	0.0	19.41989	25
AGAAGAG	950	0.0	19.08421	20
CGTGCCA	555	0.0	19.0	10
>>END_MODULE