FastQCFastQC Report
Fri 10 Feb 2017
ERR1631747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178893
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3070.17161096297786943No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2710.15148720184691408No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2680.14981022175266778No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2440.13639438099869755No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2240.12521451370372236No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2070.11571162650299341No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2060.11515263313824466No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1940.10844471276125953No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1910.10676773266701325No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1850.10341377247852068No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1820.1017367923842744No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1820.1017367923842744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCT200.00183778437.04
GGTATCA1950.032.2564131
GCGTAGT250.005484096729.59999820
TAGTCGA250.005484096729.59999823
AGTCGAT250.005484096729.59999824
CAGGACC358.840022E-426.428574
TAGGAGT400.001924852323.1254
CTGTAGA450.00381337820.5555571
TATACAC555.119792E-420.18181837
GAATAGG656.860571E-519.9230771
CGCTACA759.194246E-619.7333342
TACACCA759.194246E-619.7333345
CTTATAC2000.019.42537
GTATCAA3300.019.0606062
TGTATCG902.1316173E-618.55
TCGATGG609.1953116E-418.526
GTATCGC902.1316173E-618.56
ACATCTA801.6042692E-518.530
ATTGCCG801.6042692E-518.511
GGCCGAA500.00701224718.49999811
Produced by FastQC (version 0.11.5)