FastQCFastQC Report
Fri 10 Feb 2017
ERR1631746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98153
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5940.6051776308416452No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5170.526728678695506No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4550.46356198995445885No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4510.459486719713101No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4210.4289221929029169No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4080.41567756461850375No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4060.4136399294978248No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3970.4044705714547696No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3860.3932635782910354No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3280.3341721597913462No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3190.32500280174829094No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3120.31787107882591464No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2730.2781371939726753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2580.26285493056758324No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2560.2608172954469043No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2430.2475726671624912No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2400.24451621448147282No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2380.24247857936079387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.24044094424011492No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2280.23229040375739915No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2270.23127158619705968No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2250.22923395107638075No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2170.22108341059366501No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2060.20987641742993085No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2050.20885759986959135No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2050.20885759986959135No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1990.20274469450755453No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1960.19968824182653613No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1850.18848124866280197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1730.1762554379387283No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1710.17421780281804938No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1700.17319898525770988No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1700.17319898525770988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1700.17319898525770988No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG1690.17218016769737043No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1690.17218016769737043No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1670.17014253257669149No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1650.16810489745601256No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1650.16810489745601256No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1650.16810489745601256No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1650.16810489745601256No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1640.1670860798956731No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1610.16402962721465467No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1600.16301080965431522No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1570.1599543569732968No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1530.1558790867319389No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1520.15486026917159945No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1460.14874736380956263No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1460.14874736380956263No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1450.14772854624922313No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1420.14467209356820473No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1400.1426344584475258No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1400.1426344584475258No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1400.1426344584475258No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1380.14059682332684686No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1380.14059682332684686No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1350.13754037064582844No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1340.136521553085489No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1320.13448391796481005No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG1280.13040864772345215No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1260.12837101260277323No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1240.12633337748209428No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1240.12633337748209428No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1220.12429574236141533No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1200.12225810724073641No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT1190.12123928968039693No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1180.12022047212005746No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA1180.12022047212005746No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1180.12022047212005746No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1140.11614520187869957No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1120.11410756675802064No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT1110.11308874919768117No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1090.11105111407700224No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1090.11105111407700224No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1080.11003229651666276No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1060.10799466139598383No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1050.10697584383564435No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1040.10595702627530489No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA1040.10595702627530489No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1030.10493820871496543No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1030.10493820871496543No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1030.10493820871496543No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1030.10493820871496543No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1020.10391939115462594No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1020.10391939115462594No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1020.10391939115462594No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1010.10290057359428648No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC1000.10188175603394699No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT990.10086293847360753No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT990.10086293847360753No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC990.10086293847360753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGCT308.2347215E-637.034
AACCCTG401.5804162E-632.37536
ACAGCTG352.3662855E-531.71428535
CGCTTAA303.5757534E-430.83333431
TACTGTC303.5757534E-430.83333436
GGTATCA1750.029.61
CAGCTGT405.8857935E-527.75000236
GCGGTAA1500.027.13333323
TAATACG1450.026.79310227
CGCGGTA1550.026.25806422
TAACCCT508.980991E-625.89999835
GGTAATA1600.025.43750225
AACGTCA1051.8189894E-1224.66666828
TCTAAGT601.3167064E-624.66666833
TTAACCC601.3167064E-624.66666834
CGGTAAT1650.024.66666624
AATACGG1600.024.28125228
GTAACGT1150.024.13043226
GTAATAC1700.023.94117526
CTGCGGG1103.6379788E-1223.54545420
Produced by FastQC (version 0.11.5)