FastQCFastQC Report
Fri 10 Feb 2017
ERR1631745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174075
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4080.23438173201206378No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3770.21657331609938246No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3670.21082866580496912No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3620.20795634065776245No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3500.20106276030446646No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3460.19876490018670115No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2940.16889271865575184No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2810.1614246732730145No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT2800.16085020824357318No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2620.1505098377136292No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2600.14936090765474652No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2590.1487864426253052No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2500.14361625736033318No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2070.11891426109435588No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2060.11833979606491454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2030.11661640097659054No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2010.11546747091770788No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1980.1137440758293839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1068504954760879No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1800.1034037052994399No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1780.10225477524055723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTAG200.001837648737.01
GCGTACA303.587052E-430.8333322
TCGAACG303.587052E-430.83333218
GGTATCA1900.030.1842121
AACGACC250.005483696729.59999837
GTACACG358.839065E-426.428574
GTCGAAC358.839065E-426.4285717
ATGCAAG358.839065E-426.4285711
GTAACAG358.839065E-426.4285725
TACACGG358.839065E-426.428575
AGTCGAA358.839065E-426.4285716
CTAAGTA659.296309E-825.61538534
TAAGTAC659.296309E-825.61538535
TCTAAGT659.296309E-825.61538533
ATCTAAG701.9034815E-723.78571332
AGCGTAC400.00192464623.1251
CATCTAA802.6922862E-823.12531
AACGGTA400.00192464623.12521
TGCAAGT400.00192464623.12512
ACGGTAA400.00192464623.12522
Produced by FastQC (version 0.11.5)