##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174075
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44980037340227	33.0	31.0	34.0	31.0	34.0
2	32.614430561539564	34.0	31.0	34.0	31.0	34.0
3	32.6912078127244	34.0	31.0	34.0	31.0	34.0
4	36.190779836277464	37.0	35.0	37.0	35.0	37.0
5	36.08456125233376	37.0	35.0	37.0	35.0	37.0
6	36.13836564699124	37.0	35.0	37.0	35.0	37.0
7	36.12722102542008	37.0	35.0	37.0	35.0	37.0
8	36.10510412178659	37.0	35.0	37.0	35.0	37.0
9	37.93642395519173	39.0	38.0	39.0	35.0	39.0
10	37.83901192014936	39.0	38.0	39.0	35.0	39.0
11	37.91839149791756	39.0	38.0	39.0	35.0	39.0
12	37.84550911963234	39.0	38.0	39.0	35.0	39.0
13	37.916311934510986	39.0	38.0	39.0	35.0	39.0
14	39.25016803102111	40.0	39.0	41.0	36.0	41.0
15	39.253741203504234	40.0	39.0	41.0	36.0	41.0
16	39.18625305184547	40.0	39.0	41.0	36.0	41.0
17	39.14809133993968	40.0	39.0	41.0	36.0	41.0
18	39.14826942409881	40.0	39.0	41.0	36.0	41.0
19	39.176636507252624	40.0	39.0	41.0	36.0	41.0
20	39.15628895590981	40.0	39.0	41.0	36.0	41.0
21	39.12868016659486	40.0	39.0	41.0	36.0	41.0
22	39.07843171046962	40.0	39.0	41.0	36.0	41.0
23	39.036989803245724	40.0	39.0	41.0	36.0	41.0
24	39.04376849059314	40.0	39.0	41.0	35.0	41.0
25	38.98343242855091	40.0	38.0	41.0	35.0	41.0
26	38.90345540715209	40.0	38.0	41.0	35.0	41.0
27	38.79196897888841	40.0	38.0	41.0	35.0	41.0
28	38.79052132701422	40.0	38.0	41.0	35.0	41.0
29	38.75874766623582	40.0	38.0	41.0	35.0	41.0
30	38.71998851069941	40.0	38.0	41.0	35.0	41.0
31	38.656182679879365	40.0	38.0	41.0	35.0	41.0
32	38.61329886543157	40.0	38.0	41.0	35.0	41.0
33	38.541663076260235	40.0	38.0	41.0	34.0	41.0
34	38.50764325721671	40.0	38.0	41.0	34.0	41.0
35	38.413017377567144	40.0	38.0	41.0	34.0	41.0
36	38.39389343673704	40.0	38.0	41.0	34.0	41.0
37	38.354720666379436	40.0	38.0	41.0	34.0	41.0
38	38.328220594571306	40.0	38.0	41.0	34.0	41.0
39	38.25361482119776	40.0	38.0	41.0	34.0	41.0
40	37.91200919144047	40.0	37.0	41.0	33.0	41.0
41	37.99549619416918	40.0	37.0	41.0	33.0	41.0
42	37.98604624443487	40.0	37.0	41.0	33.0	41.0
43	37.49860117765331	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	1.0
19	1.0
20	2.0
21	14.0
22	21.0
23	51.0
24	85.0
25	180.0
26	306.0
27	475.0
28	713.0
29	995.0
30	1386.0
31	1881.0
32	2527.0
33	3416.0
34	5204.0
35	7859.0
36	11755.0
37	20067.0
38	45811.0
39	71322.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.90564411891426	15.753554502369669	12.442338072669825	28.898463306046246
2	21.108717506821773	18.56556082148499	32.51299727129111	27.81272440040213
3	21.333333333333336	19.275025132845037	28.893293120781273	30.498348413040354
4	15.692661209248888	15.360620422231797	33.3453970989516	35.60132126956771
5	17.254631624299872	32.56297572885251	33.12480252764613	17.057590119201492
6	35.11072813442482	33.05759011920149	15.285365503374981	16.546316242998707
7	30.897027143472638	27.210110584518166	19.755277897457994	22.1375843745512
8	27.9931064196467	30.716645124228066	19.654172052276316	21.636076403848918
9	29.058164584230934	12.371104409019102	17.074249605055293	41.49648140169467
10	19.839149791756427	22.87117621714778	29.109291971851214	28.180382019244576
11	39.70357604480827	19.010196754272584	18.19560534252477	23.09062185839437
12	24.166020393508543	23.190578773517164	25.448800804251043	27.194600028723254
13	34.5420077552779	17.159270429412608	20.61123079132558	27.687491023983913
14	24.184403274450666	19.438173201206375	23.07798362774666	33.2994398965963
15	30.29843458279477	23.09981329886543	19.91842596581933	26.683326152520465
16	26.037627459428407	23.470917707884535	21.60333189717076	28.888122935516304
17	27.21585523481258	23.53066207094643	21.458566709751544	27.794915984489442
18	26.682177222461583	20.83642108286658	23.110153669395377	29.371248025276465
19	28.50438029584949	21.81358609794629	22.570731006749963	27.111302599454255
20	29.72396955335344	20.281487864426254	22.871750682177222	27.122791900043087
21	28.64225190291541	21.47005601034037	21.494183541576906	28.39350854516731
22	29.4315668533678	22.602901048398678	20.812293551630045	27.153238546603475
23	28.928335487577193	22.081286801665946	21.23682320838719	27.75355450236967
24	28.693379290535688	21.304035616831825	21.968117190866007	28.03446790176648
25	28.167743788596873	21.316099382450094	22.932069510268562	27.584087318684475
26	28.49116760017234	22.279477236823208	22.38747666235818	26.84187850064627
27	28.164871463449664	21.183397960649145	23.37210972282062	27.279620853080566
28	26.57302886686773	21.89975585236249	22.394944707740915	29.13227057302887
29	28.05055292259084	22.583943702427113	22.558092776102253	26.807410598879795
30	26.989516013212693	21.04322849346546	23.986212839293408	27.98104265402844
31	27.43932213126526	22.09909521757863	22.903920723825937	27.557661927330173
32	25.752118339796066	21.721097228206233	22.99583512853655	29.530949303461153
33	26.64311360045957	21.839437024271145	24.07353152376849	27.44391785150079
34	26.91368662932644	21.674565560821485	23.897170759730002	27.514577050122075
35	26.39724256785868	22.654028436018958	24.80137871607066	26.1473502800517
36	26.57877351716214	21.432141318397242	24.45325290822921	27.5358322562114
37	25.929628033893437	21.485566566135287	24.92603762745943	27.65876777251185
38	26.252477380439466	20.111446215711617	25.952606635071092	27.683469768777826
39	24.699123940830102	19.84489444205084	27.30259945425822	28.153382162860837
40	24.708889846330603	20.544018382880942	28.454976303317537	26.292115467470918
41	22.945856670975154	19.519172770357603	29.073675140025852	28.46129541864139
42	21.127100387763896	20.725549332184404	29.981904351572595	28.165445928479105
43	20.778400114893007	20.29699842022117	30.085882521901482	28.838718942984347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	6.0
4	9.0
5	9.0
6	9.0
7	9.0
8	12.5
9	16.0
10	25.0
11	34.0
12	34.0
13	74.5
14	115.0
15	219.5
16	324.0
17	315.0
18	306.0
19	306.0
20	349.0
21	392.0
22	295.5
23	199.0
24	221.5
25	244.0
26	244.0
27	281.5
28	319.0
29	472.5
30	626.0
31	772.0
32	918.0
33	918.0
34	1326.0
35	1734.0
36	2102.5
37	2471.0
38	3186.5
39	3902.0
40	3902.0
41	4616.5
42	5331.0
43	6487.5
44	7644.0
45	9996.5
46	12349.0
47	12349.0
48	13546.5
49	14744.0
50	17011.0
51	19278.0
52	19715.5
53	20153.0
54	20153.0
55	18866.0
56	17579.0
57	16241.0
58	14903.0
59	13878.0
60	12853.0
61	12853.0
62	12111.0
63	11369.0
64	9099.0
65	6829.0
66	6018.5
67	5208.0
68	5208.0
69	4438.0
70	3668.0
71	3440.5
72	3213.0
73	3327.5
74	3442.0
75	3442.0
76	2917.5
77	2393.0
78	1621.5
79	850.0
80	575.0
81	300.0
82	300.0
83	231.5
84	163.0
85	128.5
86	94.0
87	71.0
88	48.0
89	48.0
90	41.0
91	34.0
92	20.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	174075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.54947580066064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.00686410437075	42.46502944133276
2	6.845921312816783	6.921154674709178
3	2.6865468100097734	4.074105988797932
4	1.4239607245948587	2.87921872755996
5	0.8977884855785622	2.2691368662932643
6	0.6557265268086461	1.988797931925894
7	0.5068527399595427	1.7934798219158408
8	0.3443416596586131	1.3925032313657906
9	0.2886560447303225	1.3132270573028868
>10	2.044457576652954	20.332040787017092
>50	0.20569584289837944	6.8953037483843165
>100	0.09318817192081277	7.676001723395089
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	408	0.23438173201206378	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	377	0.21657331609938246	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	367	0.21082866580496912	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	362	0.20795634065776245	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	350	0.20106276030446646	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	346	0.19876490018670115	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	294	0.16889271865575184	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	281	0.1614246732730145	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	280	0.16085020824357318	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	262	0.1505098377136292	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	260	0.14936090765474652	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	259	0.1487864426253052	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	250	0.14361625736033318	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	207	0.11891426109435588	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	206	0.11833979606491454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	203	0.11661640097659054	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	201	0.11546747091770788	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	198	0.1137440758293839	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.1068504954760879	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	180	0.1034037052994399	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	178	0.10225477524055723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.744650294413328E-4	0.0	0.0	0.0	0.0
3	5.744650294413328E-4	0.0	0.0	0.0	0.0
4	5.744650294413328E-4	0.0	0.0	0.0	0.0
5	5.744650294413328E-4	0.0	0.0	0.0	0.0
6	5.744650294413328E-4	0.0	0.0	0.0	0.0
7	5.744650294413328E-4	0.0	0.0	0.0	0.0
8	5.744650294413328E-4	0.0	0.0	0.0	0.0
9	5.744650294413328E-4	0.0	0.0	0.0	0.0
10	5.744650294413328E-4	0.0	0.0	0.0	0.0
11	5.744650294413328E-4	0.0	0.0	0.0	0.0
12	5.744650294413328E-4	0.0	0.0	0.0	0.0
13	5.744650294413328E-4	0.0	0.0	0.0	0.0
14	5.744650294413328E-4	0.0	0.0	0.0	0.0
15	5.744650294413328E-4	0.0	0.0	0.0	0.0
16	5.744650294413328E-4	0.0	0.0	0.0	0.0
17	5.744650294413328E-4	0.0	0.0	0.0	0.0
18	5.744650294413328E-4	0.0	0.0	0.0	0.0
19	5.744650294413328E-4	0.0	0.0	0.0	0.0
20	5.744650294413328E-4	0.0	0.0	0.0011489300588826655	0.0
21	5.744650294413328E-4	0.0	0.0	0.0034467901766479965	0.0
22	5.744650294413328E-4	0.0	0.0	0.005170185264971995	0.0
23	5.744650294413328E-4	0.0	0.0	0.007468045382737326	0.0
24	5.744650294413328E-4	0.0	0.0	0.008042510412178659	0.0
25	5.744650294413328E-4	0.0	0.0	0.013212695677150653	0.0
26	5.744650294413328E-4	0.0	0.0	0.018957345971563982	0.0
27	5.744650294413328E-4	0.0	0.0	0.044233807266982623	0.0
28	0.0011489300588826655	0.0	0.0	0.18210541433290248	0.0
29	0.0011489300588826655	0.0	0.0	0.38374263966681027	0.0
30	0.0011489300588826655	0.0	0.0	0.67671980468189	0.0
31	0.0011489300588826655	0.0	0.0	1.4200775527789746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTAG	20	0.0018376487	37.0	1
GCGTACA	30	3.587052E-4	30.833332	2
TCGAACG	30	3.587052E-4	30.833332	18
GGTATCA	190	0.0	30.184212	1
AACGACC	25	0.0054836967	29.599998	37
GTACACG	35	8.839065E-4	26.42857	4
GTCGAAC	35	8.839065E-4	26.42857	17
ATGCAAG	35	8.839065E-4	26.42857	11
GTAACAG	35	8.839065E-4	26.42857	25
TACACGG	35	8.839065E-4	26.42857	5
AGTCGAA	35	8.839065E-4	26.42857	16
CTAAGTA	65	9.296309E-8	25.615385	34
TAAGTAC	65	9.296309E-8	25.615385	35
TCTAAGT	65	9.296309E-8	25.615385	33
ATCTAAG	70	1.9034815E-7	23.785713	32
AGCGTAC	40	0.001924646	23.125	1
CATCTAA	80	2.6922862E-8	23.125	31
AACGGTA	40	0.001924646	23.125	21
TGCAAGT	40	0.001924646	23.125	12
ACGGTAA	40	0.001924646	23.125	22
>>END_MODULE