FastQCFastQC Report
Fri 10 Feb 2017
ERR1631744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473141
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8980.18979543096032683No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8800.1859910682016566No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8370.17690286827816656No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7570.15999458935074323No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7420.15682428705185134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.1559788731054802No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7330.1549221056725162No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6860.14498849180265502No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6530.13801382674509288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6480.13695705931212893No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6430.13590029187916497No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6300.13315269655345868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5970.12617803149589657No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5880.12427585011656145No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5700.1204714873578912No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5290.11180599440758675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.028.5359481
TAACCGT358.861651E-426.4285727
TCTGTAC358.861651E-426.4285723
GTACTAT358.861651E-426.4285721
CAGTCGG1350.026.03703510
GCAGTCG1450.024.241389
TATTAGA851.9699655E-923.9411772
CTAACCG400.001929516823.1250026
CTTATTG1600.023.12500228
GTATAGA400.001929516823.1250021
AGTCGGT1650.022.42424211
ATACGGC753.7311474E-722.229
GTGCTAG502.6988474E-422.1999991
CTAAGTC603.7203237E-521.58333234
TAAGTCT603.7203237E-521.58333235
ATATGCC1201.0913936E-1121.58333235
GATATGC1301.8189894E-1221.34615134
CCGCTTA705.0914587E-621.14285925
ATTAGAG909.4487405E-820.5555573
ATCGTCT450.003822530420.5555574
Produced by FastQC (version 0.11.5)