##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473141
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59435348025219	34.0	31.0	34.0	31.0	34.0
2	32.75788824050336	34.0	31.0	34.0	31.0	34.0
3	32.84842996062485	34.0	31.0	34.0	31.0	34.0
4	36.28467201109183	37.0	37.0	37.0	35.0	37.0
5	36.20148116523404	37.0	35.0	37.0	35.0	37.0
6	36.25609490616962	37.0	37.0	37.0	35.0	37.0
7	36.2411839176905	37.0	37.0	37.0	35.0	37.0
8	36.21688883440666	37.0	36.0	37.0	35.0	37.0
9	38.05121517687117	39.0	38.0	39.0	35.0	39.0
10	37.98791480763662	39.0	38.0	39.0	35.0	39.0
11	38.05614816724824	39.0	38.0	39.0	35.0	39.0
12	37.989214631579166	39.0	38.0	39.0	35.0	39.0
13	38.0457728245914	39.0	38.0	39.0	35.0	39.0
14	39.42239417002543	40.0	39.0	41.0	37.0	41.0
15	39.43435889090144	40.0	39.0	41.0	37.0	41.0
16	39.379009639832525	40.0	39.0	41.0	37.0	41.0
17	39.35016622951721	40.0	39.0	41.0	36.0	41.0
18	39.37165665203396	40.0	39.0	41.0	36.0	41.0
19	39.39872892013163	40.0	39.0	41.0	37.0	41.0
20	39.38781251254911	40.0	39.0	41.0	37.0	41.0
21	39.3581004394039	40.0	39.0	41.0	36.0	41.0
22	39.329743142107745	40.0	39.0	41.0	36.0	41.0
23	39.26813571430081	40.0	39.0	41.0	36.0	41.0
24	39.27579939172467	40.0	39.0	41.0	36.0	41.0
25	39.21901082341205	40.0	39.0	41.0	36.0	41.0
26	39.16178686691705	40.0	39.0	41.0	36.0	41.0
27	39.045810868218986	40.0	39.0	41.0	35.0	41.0
28	39.039455468877144	40.0	39.0	41.0	35.0	41.0
29	39.01780653124544	40.0	39.0	41.0	35.0	41.0
30	38.974758053096224	40.0	38.0	41.0	35.0	41.0
31	38.892837441692855	40.0	38.0	41.0	35.0	41.0
32	38.84962833489383	40.0	38.0	41.0	35.0	41.0
33	38.78669994779569	40.0	38.0	41.0	35.0	41.0
34	38.75978619481296	40.0	38.0	41.0	35.0	41.0
35	38.68465003032922	40.0	38.0	41.0	35.0	41.0
36	38.6440025277877	40.0	38.0	41.0	35.0	41.0
37	38.605565782715935	40.0	38.0	41.0	35.0	41.0
38	38.5698132269239	40.0	38.0	41.0	35.0	41.0
39	38.495945183359716	40.0	38.0	41.0	35.0	41.0
40	38.162579442491776	40.0	38.0	41.0	34.0	41.0
41	38.24856015437258	40.0	37.0	41.0	34.0	41.0
42	38.24053928955639	40.0	37.0	41.0	34.0	41.0
43	37.762223100513374	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	5.0
18	4.0
19	12.0
20	19.0
21	48.0
22	76.0
23	134.0
24	220.0
25	350.0
26	633.0
27	995.0
28	1472.0
29	2200.0
30	3142.0
31	4429.0
32	5976.0
33	8317.0
34	12099.0
35	18058.0
36	27608.0
37	50086.0
38	114333.0
39	222924.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.87876129948578	16.515161442360736	12.860225598711589	27.7458516594419
2	20.37447610754511	19.44409805956364	33.20426680418734	26.977159028703916
3	20.80394639230166	20.077524458882237	29.335652585592882	29.782876563223226
4	15.496014930010293	16.075334836761137	34.042283378527756	34.38636685470082
5	16.763501789107263	32.891674997516596	33.53397824327209	16.81084497010405
6	33.78337535745158	33.827970943122665	15.716245263039982	16.672408436385773
7	30.183180066829973	28.282478161900997	20.035042408077086	21.499299363191945
8	27.19844612916657	31.21500778837598	20.241534764478242	21.345011317979207
9	28.221608357762275	13.249116014042325	18.04768557364507	40.48159005455034
10	19.14482152254825	23.987775314335472	29.897430152956517	26.969973010159766
11	38.30316966823843	20.066111370606222	19.16933852699301	22.461380434162333
12	22.774183594319663	24.092395290198905	27.113904734529452	26.01951638095198
13	34.24137836289816	17.992310960157752	21.623363859821914	26.14294681712217
14	23.85821562705409	20.36623332156799	23.559995857471662	32.21555519390626
15	28.923513286736934	24.465011487061997	20.620068858965933	25.991406367235136
16	25.34318522385505	24.504745942541444	22.83357392405224	27.318494909551276
17	26.287089894978454	24.145445015333696	22.468355099219895	27.099109990467955
18	26.1421014031758	22.02662631224096	24.038711504604336	27.79256077997891
19	27.69195652036074	22.592630949336456	24.029623304680843	25.685789225621956
20	29.034051160224966	21.39911781054696	23.531463136781635	26.035367892446438
21	27.091712618437207	22.738042148112296	22.58142921454704	27.588816018903454
22	27.663001092697527	23.30552625961394	22.219380692013583	26.812091955674948
23	27.438120982962793	22.4886450339328	23.21696914873156	26.85626483437284
24	27.274110677366792	22.335413756153027	23.378443212488456	27.012032353991728
25	27.306236407328893	22.555432735696122	23.618752126744457	26.519578730230524
26	27.10058946487411	23.190761316394056	23.14997009348165	26.55867912525019
27	26.557199650844037	22.324634728336797	24.49100796591291	26.627157654906252
28	25.877064130988437	23.433395119002583	23.051690722215998	27.637850027792982
29	26.68675933812542	23.785721381152765	23.18251853041694	26.345000750304877
30	26.30061651812039	22.738887562058668	24.26464838177203	26.695847538048913
31	27.155118664415046	22.893386960757997	23.36216899402081	26.589325380806144
32	25.126547900097435	22.833362570565647	23.85039554805016	28.189693981286762
33	25.47042002278391	22.376839039525215	25.21193470868092	26.940806229009954
34	25.939202056046717	22.205431361898462	24.75667929856005	27.098687283494773
35	25.405111795426734	23.45453046766186	25.084277202778875	26.05608053413253
36	25.512902073589057	22.170980743583836	25.740529778649496	26.575587404177615
37	25.813658085010598	21.69585810572324	25.460909115887233	27.029574693378926
38	25.10583525841134	21.154581826559102	26.508376995441107	27.231205919588454
39	24.657343159861437	20.513758055209756	27.532807344956368	27.296091439972443
40	24.227027461158514	21.43990903345937	28.18039442787668	26.152669077505436
41	22.673367981214902	20.769707127473627	29.161497312640417	27.39542757867105
42	21.458930847252724	21.356213052768624	29.71756833586605	27.4672877641126
43	20.899689521728195	21.8260518534644	29.49712664934977	27.777131975457632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	24.0
4	43.0
5	43.0
6	54.0
7	65.0
8	69.5
9	74.0
10	109.0
11	144.0
12	144.0
13	319.5
14	495.0
15	919.0
16	1343.0
17	1332.0
18	1321.0
19	1321.0
20	1476.5
21	1632.0
22	1461.0
23	1290.0
24	1465.5
25	1641.0
26	1641.0
27	1888.5
28	2136.0
29	2825.5
30	3515.0
31	4174.5
32	4834.0
33	4834.0
34	5968.0
35	7102.0
36	8212.0
37	9322.0
38	11420.5
39	13519.0
40	13519.0
41	15791.0
42	18063.0
43	20710.0
44	23357.0
45	29182.0
46	35007.0
47	35007.0
48	38718.5
49	42430.0
50	47472.5
51	52515.0
52	53062.5
53	53610.0
54	53610.0
55	48659.5
56	43709.0
57	40754.0
58	37799.0
59	35118.0
60	32437.0
61	32437.0
62	29580.0
63	26723.0
64	22420.5
65	18118.0
66	15735.0
67	13352.0
68	13352.0
69	11420.5
70	9489.0
71	8114.0
72	6739.0
73	5494.0
74	4249.0
75	4249.0
76	3463.0
77	2677.0
78	2195.0
79	1713.0
80	1398.5
81	1084.0
82	1084.0
83	880.0
84	676.0
85	556.5
86	437.0
87	352.0
88	267.0
89	267.0
90	209.0
91	151.0
92	99.5
93	48.0
94	27.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	473141.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.429815610711294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54474555107566	47.10614542420975
2	6.48116441198726	7.0553716777434134
3	2.1678039273479324	3.539795041371713
4	1.0706662712722184	2.331046709038186
5	0.6478362044042787	1.7630802575833981
6	0.4691481382687822	1.5321387996046982
7	0.35109166156200833	1.337689808089486
8	0.2795093086805324	1.2170912106367011
9	0.21159712333363306	1.0365473166125927
>10	1.5261538817686844	16.329407183358104
>50	0.1581354535474511	5.9502895213681475
>100	0.0859007401921328	8.436200533507472
>500	0.00624732655942784	2.3651965168762983
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	898	0.18979543096032683	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	880	0.1859910682016566	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	837	0.17690286827816656	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	757	0.15999458935074323	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	742	0.15682428705185134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.1559788731054802	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	733	0.1549221056725162	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	686	0.14498849180265502	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	653	0.13801382674509288	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.13695705931212893	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	643	0.13590029187916497	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	630	0.13315269655345868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.12617803149589657	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	588	0.12427585011656145	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	570	0.1204714873578912	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	529	0.11180599440758675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1135348659279157E-4	0.0
2	0.0	0.0	0.0	2.1135348659279157E-4	0.0
3	0.0	0.0	0.0	2.1135348659279157E-4	0.0
4	0.0	0.0	0.0	2.1135348659279157E-4	0.0
5	0.0	0.0	0.0	2.1135348659279157E-4	0.0
6	0.0	0.0	0.0	2.1135348659279157E-4	0.0
7	0.0	0.0	0.0	2.1135348659279157E-4	0.0
8	0.0	0.0	0.0	2.1135348659279157E-4	0.0
9	0.0	0.0	0.0	2.1135348659279157E-4	0.0
10	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
11	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
12	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
13	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
14	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
15	0.0	0.0	2.1135348659279157E-4	2.1135348659279157E-4	0.0
16	0.0	0.0	2.1135348659279157E-4	4.2270697318558313E-4	0.0
17	0.0	0.0	2.1135348659279157E-4	8.454139463711663E-4	0.0
18	0.0	0.0	2.1135348659279157E-4	0.0012681209195567495	0.0
19	0.0	0.0	2.1135348659279157E-4	0.0016908278927423325	0.0
20	0.0	0.0	2.1135348659279157E-4	0.0023248883525207073	0.0
21	2.1135348659279157E-4	0.0	2.1135348659279157E-4	0.004438423218448623	0.0
22	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.007397372030747705	0.0
23	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.008876846436897247	0.0
24	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.012469855708974704	0.0
25	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.014160683601717036	0.0
26	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.019655874253129618	0.0
27	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.04142528337218715	0.0
28	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.1477360871283613	0.0
29	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.3259070763260846	0.0
30	4.2270697318558313E-4	0.0	2.1135348659279157E-4	0.5518439534937788	0.0
31	4.2270697318558313E-4	0.0	2.1135348659279157E-4	1.23409300821531	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	765	0.0	28.535948	1
TAACCGT	35	8.861651E-4	26.428572	7
TCTGTAC	35	8.861651E-4	26.428572	3
GTACTAT	35	8.861651E-4	26.428572	1
CAGTCGG	135	0.0	26.037035	10
GCAGTCG	145	0.0	24.24138	9
TATTAGA	85	1.9699655E-9	23.941177	2
CTAACCG	40	0.0019295168	23.125002	6
CTTATTG	160	0.0	23.125002	28
GTATAGA	40	0.0019295168	23.125002	1
AGTCGGT	165	0.0	22.424242	11
ATACGGC	75	3.7311474E-7	22.2	29
GTGCTAG	50	2.6988474E-4	22.199999	1
CTAAGTC	60	3.7203237E-5	21.583332	34
TAAGTCT	60	3.7203237E-5	21.583332	35
ATATGCC	120	1.0913936E-11	21.583332	35
GATATGC	130	1.8189894E-12	21.346151	34
CCGCTTA	70	5.0914587E-6	21.142859	25
ATTAGAG	90	9.4487405E-8	20.555557	3
ATCGTCT	45	0.0038225304	20.555557	4
>>END_MODULE