FastQCFastQC Report
Fri 10 Feb 2017
ERR1631743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156391
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4630.2960528419154555No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4390.2807066902826889No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4290.27431246043570284No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4260.272394191481607No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3970.2538509249253474No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3570.22827400553740307No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3390.2167643918128281No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3290.21037016196584205No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3080.19694227928717126No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3070.19630285630247266No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2990.1911874724248838No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2910.18607208854729493No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2240.1432307485724882No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2230.14259132558778956No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2120.1355576727561049No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2120.1355576727561049No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2030.1298028658938174No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2010.1285240199244202No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2000.1278845969397216No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1970.1259663279856258No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1880.12021152112333829No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1840.11765382918454387No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1770.11317786829165362No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1710.10934133038346197No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1690.10806248441406474No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1610.10294710053647589No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1570.10038940859768146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATT303.5853966E-430.83333226
TAGAGCT250.005482011529.59999833
CTGGTAC250.005482011529.5999983
AGCGCAA250.005482011529.59999810
TGAATAG250.005482011529.5999985
GGTATCA2700.028.7777791
CCCCGAT358.835021E-426.42857225
TTAACGT358.835021E-426.42857231
CGACTCA358.835021E-426.42857213
ATTGCCG358.835021E-426.42857211
CTAATAC358.835021E-426.4285723
ATGTGTA400.001923774623.1257
ATACGGC400.001923774623.12529
AAAGACT400.001923774623.12536
CTCTATG1250.022.1999991
AGCGTAC603.6998506E-521.5833321
GTACACG603.6998506E-521.5833324
TCTATGG1201.0913936E-1121.5833322
TAATACG1850.021.027
CAGTCGG806.8813097E-720.812510
Produced by FastQC (version 0.11.5)