FastQCFastQC Report
Fri 10 Feb 2017
ERR1631742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323746
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8060.2489606049186708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7130.2202343812742088No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7030.2171455400221161No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6470.19984802901039703No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6100.18841931637765408No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5770.1782261402457482No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5600.17297511011719063No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5410.16710631173821452No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5100.1575309038567272No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5080.15691313560630865No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4650.1436311182223101No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4070.12571583896017247No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3980.12293588183328906No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3960.12231811358287054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3680.11366935807701099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.10996274857449975No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3560.10996274857449975No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3310.10224064544426803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTT303.5938175E-430.83333431
GGTATCA4650.028.247311
TACCCTC358.855581E-426.428575
GTTCTAG659.348332E-825.6153851
TGTCCGA601.3324534E-624.66666812
TACGGCT701.9140498E-723.78571330
TAGGACA551.8971901E-523.5454544
ATTAGAG400.001928207723.1253
AGTGCGC400.001928207723.1253
AAGTTCG400.001928207723.12533
TTGAATA400.001928207723.1254
ACTAAGT400.001928207723.12530
CTACCCA400.001928207723.12527
TATACAC1450.022.96551737
CGCGGTA2850.022.71929722
AGCCGCG2750.022.219
AAATCTC753.7236168E-722.29
ATACGGC753.7236168E-722.229
GCGGTAA2800.021.8035723
GCTTAGG603.7156504E-521.5833341
Produced by FastQC (version 0.11.5)