##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631742.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323746
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62052967449791	34.0	31.0	34.0	31.0	34.0
2	32.78393555441612	34.0	31.0	34.0	31.0	34.0
3	32.87027484509461	34.0	31.0	34.0	31.0	34.0
4	36.30760843377215	37.0	37.0	37.0	35.0	37.0
5	36.21301575926807	37.0	35.0	37.0	35.0	37.0
6	36.26695310521211	37.0	37.0	37.0	35.0	37.0
7	36.251873382219394	37.0	37.0	37.0	35.0	37.0
8	36.22994878701204	37.0	37.0	37.0	35.0	37.0
9	38.074323698207856	39.0	38.0	39.0	37.0	39.0
10	38.00071043348798	39.0	38.0	39.0	35.0	39.0
11	38.084726915544906	39.0	38.0	39.0	37.0	39.0
12	38.013204796352696	39.0	38.0	39.0	35.0	39.0
13	38.06942170714078	39.0	38.0	39.0	35.0	39.0
14	39.45923965083738	40.0	39.0	41.0	37.0	41.0
15	39.45951455770882	41.0	39.0	41.0	37.0	41.0
16	39.40777337789502	40.0	39.0	41.0	37.0	41.0
17	39.37794443792356	40.0	39.0	41.0	37.0	41.0
18	39.39591840516948	40.0	39.0	41.0	37.0	41.0
19	39.41224602002805	40.0	39.0	41.0	37.0	41.0
20	39.41677735014487	40.0	39.0	41.0	37.0	41.0
21	39.37420385116727	40.0	39.0	41.0	37.0	41.0
22	39.3467224305474	40.0	39.0	41.0	36.0	41.0
23	39.284497723524	40.0	39.0	41.0	36.0	41.0
24	39.297588232750364	40.0	39.0	41.0	36.0	41.0
25	39.251138238001396	40.0	39.0	41.0	36.0	41.0
26	39.192456431894136	40.0	39.0	41.0	36.0	41.0
27	39.078206371661736	40.0	39.0	41.0	36.0	41.0
28	39.07806119612289	40.0	39.0	41.0	35.0	41.0
29	39.053538885422526	40.0	39.0	41.0	35.0	41.0
30	39.0231014437244	40.0	39.0	41.0	35.0	41.0
31	38.93485324915211	40.0	38.0	41.0	35.0	41.0
32	38.89322493559766	40.0	38.0	41.0	35.0	41.0
33	38.83466668314048	40.0	38.0	41.0	35.0	41.0
34	38.81276679866315	40.0	38.0	41.0	35.0	41.0
35	38.74425938853299	40.0	38.0	41.0	35.0	41.0
36	38.70572609391313	40.0	38.0	41.0	35.0	41.0
37	38.66470628208534	40.0	38.0	41.0	35.0	41.0
38	38.635828705219524	40.0	38.0	41.0	35.0	41.0
39	38.57465420422183	40.0	38.0	41.0	35.0	41.0
40	38.25092819679625	40.0	38.0	41.0	34.0	41.0
41	38.34016173172796	40.0	38.0	41.0	34.0	41.0
42	38.33615241578274	40.0	37.0	41.0	34.0	41.0
43	37.873153645141564	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	6.0
19	4.0
20	11.0
21	32.0
22	44.0
23	91.0
24	149.0
25	220.0
26	400.0
27	646.0
28	935.0
29	1465.0
30	2003.0
31	2875.0
32	3962.0
33	5586.0
34	8037.0
35	12254.0
36	18706.0
37	33479.0
38	76766.0
39	156071.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.266468157135535	16.200045714850532	13.25946884285829	28.274017285155644
2	20.423727242962077	19.40255632502023	33.01199088174062	27.161725550277072
3	20.710989479406695	19.798854657663725	29.56175520315309	29.928400659776493
4	15.63416999746715	16.01440635559976	33.819105100912445	34.53231854602065
5	16.744608427594475	33.02434624674899	33.41508466513872	16.815960660517813
6	33.899414973466854	33.62172814490372	15.745986050792906	16.732870830836518
7	30.090564825511358	27.881425562014666	20.299864708753155	21.728144903720818
8	27.313387655754823	31.103395872072554	20.419093981083936	21.164122491088694
9	28.4890006363013	13.049118753590777	17.80933200719082	40.6525486029171
10	18.958072068844096	24.001223181135828	29.602219023555502	27.438485726464574
11	38.75661784238261	19.630203925299465	18.95127661808949	22.661901614228437
12	22.71688298851569	24.297443057211517	26.629827086666708	26.355846867606086
13	34.623130478832174	17.718828958504506	21.38497464061332	26.273065922050005
14	24.071030993433123	20.409518573202448	23.15920505581536	32.36024537754907
15	29.233720262180846	24.526017309866376	20.30882234838423	25.931440079568553
16	25.552439257936776	24.404008080408715	22.567383071914403	27.476169589740106
17	26.91925151198779	24.05095352529452	21.730615976722493	27.299178985995194
18	26.397237340384127	21.67717902306129	23.644770900644332	28.28081273591025
19	28.09548226078469	22.545761183149754	23.523070555311882	25.835686000753675
20	29.821217868328876	21.05539527901503	23.13109660042132	25.992290252234778
21	27.175316451786273	22.655415047599043	22.00984722591167	28.159421274703007
22	28.291623680292577	22.86514736861614	21.665132542178128	27.17809640891316
23	27.628758347593486	22.362901781025865	22.842289943350654	27.16604992803
24	27.753856418303236	22.013553835414182	23.23271947761517	26.999870268667415
25	27.725747962909193	22.22019731517918	23.303762826413298	26.750291895498325
26	27.403890704441135	23.155807330438062	22.71163195838713	26.728670006733672
27	26.666275413441404	21.996565208527674	24.496673317971496	26.840486060059433
28	25.898080594045947	23.014647285217425	22.687847880746016	28.399424239990612
29	26.946742199131418	23.808788371130454	22.67765470461411	26.566814725124015
30	26.45901416542598	22.45371371383739	23.935739746591462	27.151532374145166
31	27.588912295441485	22.688465648996438	23.242603769621866	26.48001828594021
32	25.119074830268172	22.481204400981017	23.42947866537347	28.97024210337734
33	25.566956811821612	21.986989800646185	25.071815559111155	27.374237828421045
34	26.112446176941184	22.04783997331241	24.501306579849636	27.33840726989677
35	25.47212938538237	23.424536519370122	25.049575902096088	26.05375819315142
36	25.52402191841752	22.135254180746635	25.7430207631909	26.597703137644945
37	26.163103173475506	21.6308464042799	25.245717321603973	26.960333100640625
38	25.20865122657886	21.018946952240334	26.522644295219088	27.24975752596171
39	24.691270316853338	19.963181012275054	27.602194312825485	27.743354358046123
40	24.41451014066583	21.0943146787914	28.328689775317688	26.162485405225084
41	22.53433247051701	20.493535055259372	29.458279021207982	27.513853453015635
42	21.285513952295936	20.866049310261747	30.046085511481223	27.802351225961093
43	20.39685432406887	21.49215743206094	30.111568946025585	27.999419297844607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	9.0
4	16.0
5	16.0
6	20.0
7	24.0
8	25.5
9	27.0
10	56.5
11	86.0
12	86.0
13	156.0
14	226.0
15	453.5
16	681.0
17	676.0
18	671.0
19	671.0
20	746.0
21	821.0
22	738.5
23	656.0
24	728.0
25	800.0
26	800.0
27	914.5
28	1029.0
29	1559.0
30	2089.0
31	2337.5
32	2586.0
33	2586.0
34	3237.5
35	3889.0
36	4390.5
37	4892.0
38	6569.5
39	8247.0
40	8247.0
41	9856.5
42	11466.0
43	12802.0
44	14138.0
45	19404.0
46	24670.0
47	24670.0
48	27582.0
49	30494.0
50	34838.0
51	39182.0
52	39917.5
53	40653.0
54	40653.0
55	35921.5
56	31190.0
57	28890.5
58	26591.0
59	24382.5
60	22174.0
61	22174.0
62	20134.5
63	18095.0
64	14993.0
65	11891.0
66	10328.0
67	8765.0
68	8765.0
69	7423.5
70	6082.0
71	5210.5
72	4339.0
73	3497.0
74	2655.0
75	2655.0
76	2189.0
77	1723.0
78	1451.5
79	1180.0
80	932.5
81	685.0
82	685.0
83	575.0
84	465.0
85	370.0
86	275.0
87	212.5
88	150.0
89	150.0
90	122.0
91	94.0
92	65.0
93	36.0
94	21.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	323746.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.49613761691769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.88709970664087	44.228539060111096
2	6.269110656218059	6.456699701765807
3	2.243218305599615	3.4655123570984014
4	1.1754512064531262	2.421247883579285
5	0.7802348953370777	2.0089541771899766
6	0.5266661640693617	1.6272763957854004
7	0.38269605733722745	1.3795158183862783
8	0.3199842300692211	1.3182361557510451
9	0.25539958533190443	1.1836882974199916
>10	1.8766991122951877	19.202469272184253
>50	0.18555717144141085	6.617709431001673
>100	0.09187782275254323	8.180602116248517
>500	0.006005086454414591	1.9095493334782863
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	806	0.2489606049186708	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	713	0.2202343812742088	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	703	0.2171455400221161	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	647	0.19984802901039703	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	610	0.18841931637765408	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	577	0.1782261402457482	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	560	0.17297511011719063	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	541	0.16710631173821452	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	510	0.1575309038567272	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	508	0.15691313560630865	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	465	0.1436311182223101	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	407	0.12571583896017247	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	398	0.12293588183328906	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	396	0.12231811358287054	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.11366935807701099	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.10996274857449975	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	356	0.10996274857449975	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	331	0.10224064544426803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	3.08884125209269E-4	0.0	0.0	0.0	0.0
13	3.08884125209269E-4	0.0	0.0	0.0	0.0
14	3.08884125209269E-4	0.0	0.0	0.0	0.0
15	3.08884125209269E-4	0.0	0.0	9.26652375627807E-4	0.0
16	3.08884125209269E-4	0.0	0.0	0.002162188876464883	0.0
17	6.17768250418538E-4	0.0	0.0	0.004633261878139035	0.0
18	9.26652375627807E-4	0.0	0.0	0.004633261878139035	0.0
19	9.26652375627807E-4	0.0	0.0	0.005251030128557573	0.0
20	9.26652375627807E-4	0.0	0.0	0.0061776825041853795	0.0
21	9.26652375627807E-4	0.0	0.0	0.00926652375627807	0.0
22	9.26652375627807E-4	0.0	0.0	0.012664249133580028	0.0
23	9.26652375627807E-4	0.0	0.0	0.016679742761300527	0.0
24	9.26652375627807E-4	0.0	0.0	0.019768584013393216	0.0
25	9.26652375627807E-4	0.0	0.0	0.021930772889858098	0.0
26	9.26652375627807E-4	0.0	0.0	0.02749068714362494	0.0
27	9.26652375627807E-4	0.0	0.0	0.05034811240911084	0.0
28	9.26652375627807E-4	0.0	0.0	0.17760837199532967	0.0
29	9.26652375627807E-4	0.0	0.0	0.3746764438788433	0.0
30	9.26652375627807E-4	0.0	0.0	0.6146794091664453	0.0
31	9.26652375627807E-4	0.0	0.0	1.3053443131343707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGTT	30	3.5938175E-4	30.833334	31
GGTATCA	465	0.0	28.24731	1
TACCCTC	35	8.855581E-4	26.42857	5
GTTCTAG	65	9.348332E-8	25.615385	1
TGTCCGA	60	1.3324534E-6	24.666668	12
TACGGCT	70	1.9140498E-7	23.785713	30
TAGGACA	55	1.8971901E-5	23.545454	4
ATTAGAG	40	0.0019282077	23.125	3
AGTGCGC	40	0.0019282077	23.125	3
AAGTTCG	40	0.0019282077	23.125	33
TTGAATA	40	0.0019282077	23.125	4
ACTAAGT	40	0.0019282077	23.125	30
CTACCCA	40	0.0019282077	23.125	27
TATACAC	145	0.0	22.965517	37
CGCGGTA	285	0.0	22.719297	22
AGCCGCG	275	0.0	22.2	19
AAATCTC	75	3.7236168E-7	22.2	9
ATACGGC	75	3.7236168E-7	22.2	29
GCGGTAA	280	0.0	21.80357	23
GCTTAGG	60	3.7156504E-5	21.583334	1
>>END_MODULE