FastQCFastQC Report
Fri 10 Feb 2017
ERR1631741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692017
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25650.3706556341823973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23830.344355702244309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20320.29363440493513887No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18570.26834600884082327No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC10970.15852211723122409No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA10880.15722157114637356No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10840.15664355066421778No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10010.14464962565948525No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9800.14161501812816737No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9410.13597931842714844No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9260.13381174161906428No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9210.13308921601636955No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9120.13178866993151903No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8710.1258639599894222No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8700.12571945486888328No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8700.12571945486888328No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8540.12340737294026013No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8370.12095078589109806No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7940.11473706570792337No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7540.1089568608863655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.031.479841
GTATAGA250.005494851629.5999981
TACCGCT358.865814E-426.42857235
ATACGGC601.3361223E-624.66666629
GTTCCGC502.700597E-422.1999991
TACGGCT705.097083E-621.14285930
GTATCAA18650.020.9302942
TAGCCCA450.003824293420.5555554
GTAACGT3000.020.3526
GCTGATA555.140252E-420.1818188
GTACTGG5500.020.1818181
TGCGGGT3050.020.01639221
CTTATAC9050.018.8066337
AACGTCA3350.018.7761228
TACTGGT5750.018.660872
CGGGTAA3350.018.2238823
GGTAACG3350.018.2238825
GCGGGTA3350.018.2238822
TGGTTCA5950.017.722695
TTCTGCG3450.017.69565218
Produced by FastQC (version 0.11.5)