FastQCFastQC Report
Fri 10 Feb 2017
ERR1631740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309498
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9030.2917627900664948No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8220.2655913770040517No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7200.23263478277727156No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6930.2239109784231239No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6660.21518717406897622No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6630.2142178624740709No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6210.20064750014539673No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5970.19289300738615436No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5880.18998507260143846No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5800.187400241681691No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4930.15929020542943734No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4510.14571984310076316No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4410.14248880445107887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.1273029227975625No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3870.1250411957427835No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3830.12374878028290975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3800.12277946868800445No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3550.11470187206379363No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3490.11276324887398302No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3290.10630117157461438No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3190.10307013292493006No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3190.10307013292493006No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3170.10242392519499317No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3160.10210082133002477No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3140.10145461360008788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT200.001839858237.013
TCTACGC200.001839858237.010
TAAGCGG308.292356E-637.018
CGCATTT250.005490215529.614
TACGCAT250.005490215529.612
CTACGCA250.005490215529.611
TTACGAG250.005490215529.66
TTTTACG250.005490215529.64
GGTATCA5150.028.7378641
TAGCGAA551.8968398E-523.54545410
GTAGCGA551.8968398E-523.5454549
AGCGAAA551.8968398E-523.54545411
TGTCGGG551.8968398E-523.54545423
GATCTCT400.001928016523.1250026
AGCGGAG502.6959236E-422.220
AACGACC502.6959236E-422.237
TATAAGA502.6959236E-422.213
AATAAGC502.6959236E-422.216
GGTAAGT603.7149708E-521.58333228
GCGAAAT603.7149708E-521.58333212
Produced by FastQC (version 0.11.5)