##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309498
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63294754731856	34.0	31.0	34.0	31.0	34.0
2	32.7963864063742	34.0	31.0	34.0	31.0	34.0
3	32.880936225759136	34.0	31.0	34.0	31.0	34.0
4	36.312732230902945	37.0	37.0	37.0	35.0	37.0
5	36.22648934726557	37.0	35.0	37.0	35.0	37.0
6	36.28130714899611	37.0	37.0	37.0	35.0	37.0
7	36.26673839572469	37.0	37.0	37.0	35.0	37.0
8	36.24931663532559	37.0	37.0	37.0	35.0	37.0
9	38.086533677115845	39.0	38.0	39.0	37.0	39.0
10	38.01542497851359	39.0	38.0	39.0	35.0	39.0
11	38.0987954687914	39.0	38.0	39.0	37.0	39.0
12	38.03150908891172	39.0	38.0	39.0	35.0	39.0
13	38.09213306709575	39.0	38.0	39.0	37.0	39.0
14	39.47594814829175	40.0	39.0	41.0	37.0	41.0
15	39.48148614853731	41.0	39.0	41.0	37.0	41.0
16	39.422997886900724	40.0	39.0	41.0	37.0	41.0
17	39.405440422878335	40.0	39.0	41.0	37.0	41.0
18	39.426739429657054	40.0	39.0	41.0	37.0	41.0
19	39.44593826131348	41.0	39.0	41.0	37.0	41.0
20	39.448855889214144	41.0	39.0	41.0	37.0	41.0
21	39.40811895391893	40.0	39.0	41.0	37.0	41.0
22	39.38511395873317	40.0	39.0	41.0	37.0	41.0
23	39.32061596520818	40.0	39.0	41.0	36.0	41.0
24	39.33088420603687	40.0	39.0	41.0	36.0	41.0
25	39.28081603112136	40.0	39.0	41.0	36.0	41.0
26	39.224583034462256	40.0	39.0	41.0	36.0	41.0
27	39.11296680430891	40.0	39.0	41.0	36.0	41.0
28	39.11582627351388	40.0	39.0	41.0	36.0	41.0
29	39.08768392687514	40.0	39.0	41.0	35.0	41.0
30	39.0527208576469	40.0	39.0	41.0	35.0	41.0
31	38.96338910106043	40.0	38.0	41.0	35.0	41.0
32	38.923728101635554	40.0	38.0	41.0	35.0	41.0
33	38.86448054591629	40.0	38.0	41.0	35.0	41.0
34	38.844994151820046	40.0	38.0	41.0	35.0	41.0
35	38.775963011069535	40.0	38.0	41.0	35.0	41.0
36	38.724925524559126	40.0	38.0	41.0	35.0	41.0
37	38.67902862054036	40.0	38.0	41.0	35.0	41.0
38	38.645076866409475	40.0	38.0	41.0	35.0	41.0
39	38.57381307795204	40.0	38.0	41.0	35.0	41.0
40	38.24668010778745	40.0	38.0	41.0	34.0	41.0
41	38.327278366903826	40.0	38.0	41.0	34.0	41.0
42	38.315514155180324	40.0	37.0	41.0	34.0	41.0
43	37.84535279711016	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	5.0
20	8.0
21	16.0
22	42.0
23	78.0
24	127.0
25	218.0
26	343.0
27	528.0
28	873.0
29	1328.0
30	1964.0
31	2657.0
32	3740.0
33	5072.0
34	7602.0
35	11548.0
36	18000.0
37	31938.0
38	73694.0
39	149713.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.69106747054908	16.211413320926145	13.024963004607462	28.07255620391731
2	20.57493101732483	19.376538782156913	32.83284544649723	27.21568475402103
3	20.96685600553154	19.77104860128337	29.361094417411422	29.901000975773673
4	15.543880735901364	15.972962668579441	34.09488914306393	34.38826745245527
5	16.793323381734293	32.61281171445373	33.70942623215659	16.884438671655392
6	33.90587338205739	33.45062003631688	15.870538743384449	16.772967838241282
7	30.093247775429887	27.91132737529806	20.135186657102793	21.860238192169255
8	27.47739888464546	30.676127147832943	20.435673251523433	21.410800715998164
9	28.599215503815856	12.795559260479875	17.91190896225501	40.69331627344926
10	19.34164356474032	23.62244667170709	29.543971204983556	27.49193855856904
11	38.91107535428338	19.479932018946812	18.781381462885058	22.827611163884743
12	22.676398555079516	24.031173060892154	26.611803630395027	26.680624753633303
13	35.05967728385967	17.80173054430077	20.878648650395156	26.259943521444402
14	24.09223969137119	20.411117357785834	23.313236272932297	32.18340667791068
15	29.75301940561813	24.070591732418304	20.094798673981735	26.081590187981828
16	25.31454161254677	24.167845995773803	22.847320499647815	27.670291892031614
17	26.781433159503454	23.85863559699901	22.041176356551578	27.318754886945957
18	26.682886480688083	21.386244822260565	23.540055186140137	28.390813510911215
19	28.040892025150406	22.446348603221992	23.52486930448662	25.987890067140984
20	29.92394135018643	20.732282599564456	23.177532649645556	26.166243400603555
21	27.255749633277116	22.389482322987547	22.03794531790189	28.316822725833447
22	28.507130902299853	22.672844412564864	21.639235148530847	27.18078953660444
23	27.83765969408526	22.21791417068931	22.586252576753324	27.358173558472103
24	27.581115225300323	21.82857401340235	23.4298767681859	27.160433993111425
25	27.764638220602393	22.004665619810144	23.278341055515707	26.952355104071756
26	27.612133196337297	22.877046055224913	22.5946532772425	26.91616747119529
27	26.67287026087406	21.79723293850041	24.59078895501748	26.939107845608053
28	26.041202204860774	22.888354690498808	22.415007528320054	28.655435576320365
29	27.222146831320398	23.468972335847084	22.776237649354762	26.53264318347776
30	26.791449379317477	22.06120879617962	23.851204208104736	27.29613761639817
31	27.773362024956544	22.60725432797627	23.026643144705297	26.592740502361888
32	25.70194314664392	22.35167917078624	23.090940813834017	28.855436868735822
33	25.878357856916683	21.784631887766643	25.003715694447138	27.333294560869536
34	26.36042882344959	21.752321501269797	24.293210295381552	27.59403937989906
35	25.47770906435583	23.534239316570705	24.65056317003664	26.337488449036826
36	25.879973376241526	22.28608908619765	25.286431576294515	26.54750596126631
37	26.440235478096792	22.001111477295492	24.847656527667382	26.710996516940334
38	25.709051431673224	21.39561483434465	25.87641923372687	27.018914500255253
39	25.20727112937725	20.413055980975646	26.72036652902442	27.659306360622686
40	24.736508798118244	21.51677878370781	27.40114637251291	26.34556604566104
41	23.21759752890164	20.74456054643326	28.39630627661568	27.64153564804942
42	21.76653807132841	21.13551622304506	29.333630588889104	27.764315116737425
43	21.157164181997945	22.043761187471326	29.027651228764	27.771423401766732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	5.5
4	9.0
5	9.0
6	13.5
7	18.0
8	27.0
9	36.0
10	46.5
11	57.0
12	57.0
13	142.5
14	228.0
15	457.5
16	687.0
17	672.0
18	657.0
19	657.0
20	738.0
21	819.0
22	688.0
23	557.0
24	639.5
25	722.0
26	722.0
27	825.5
28	929.0
29	1274.0
30	1619.0
31	1966.0
32	2313.0
33	2313.0
34	2921.0
35	3529.0
36	4069.0
37	4609.0
38	5843.0
39	7077.0
40	7077.0
41	8585.0
42	10093.0
43	11687.5
44	13282.0
45	18551.5
46	23821.0
47	23821.0
48	26393.0
49	28965.0
50	32990.0
51	37015.0
52	37211.5
53	37408.0
54	37408.0
55	33638.5
56	29869.0
57	28115.0
58	26361.0
59	24259.5
60	22158.0
61	22158.0
62	20293.5
63	18429.0
64	15228.5
65	12028.0
66	10386.0
67	8744.0
68	8744.0
69	7447.0
70	6150.0
71	5290.5
72	4431.0
73	3553.5
74	2676.0
75	2676.0
76	2160.5
77	1645.0
78	1348.5
79	1052.0
80	850.0
81	648.0
82	648.0
83	526.0
84	404.0
85	318.0
86	232.0
87	183.0
88	134.0
89	134.0
90	96.0
91	58.0
92	41.0
93	24.0
94	12.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	309498.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.44305649023355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.27520454628834	47.51528891310265
2	5.843154229101755	6.362383515522679
3	1.9705062890351737	3.218411556249073
4	1.0766233991525547	2.344586741550792
5	0.6990472001597433	1.9029133103818257
6	0.48139721287153886	1.5725241392763705
7	0.33216017013894256	1.2658670434676027
8	0.27860687877929546	1.213456803195907
9	0.2077233049282117	1.0178182462090175
>10	1.5829389420316253	16.784456261731247
>50	0.1624947529072878	6.0519252265334424
>100	0.08421260917093017	8.537721871810918
>500	0.005930465434572547	2.2126463709685065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	903	0.2917627900664948	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	822	0.2655913770040517	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	720	0.23263478277727156	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	693	0.2239109784231239	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	666	0.21518717406897622	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	663	0.2142178624740709	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	621	0.20064750014539673	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	597	0.19289300738615436	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	588	0.18998507260143846	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	580	0.187400241681691	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	493	0.15929020542943734	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	451	0.14571984310076316	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	441	0.14248880445107887	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	394	0.1273029227975625	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	387	0.1250411957427835	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	383	0.12374878028290975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.12277946868800445	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	355	0.11470187206379363	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	349	0.11276324887398302	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	329	0.10630117157461438	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	319	0.10307013292493006	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	319	0.10307013292493006	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	317	0.10242392519499317	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	316	0.10210082133002477	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	314	0.10145461360008788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2310386496843275E-4
2	0.0	0.0	0.0	0.0	3.2310386496843275E-4
3	0.0	0.0	0.0	0.0	3.2310386496843275E-4
4	0.0	0.0	0.0	0.0	3.2310386496843275E-4
5	0.0	0.0	0.0	0.0	3.2310386496843275E-4
6	0.0	0.0	0.0	0.0	3.2310386496843275E-4
7	0.0	0.0	0.0	0.0	3.2310386496843275E-4
8	0.0	0.0	0.0	0.0	3.2310386496843275E-4
9	0.0	0.0	0.0	0.0	3.2310386496843275E-4
10	0.0	0.0	0.0	0.0	3.2310386496843275E-4
11	0.0	0.0	0.0	0.0	3.2310386496843275E-4
12	0.0	0.0	0.0	0.0	3.2310386496843275E-4
13	0.0	0.0	0.0	0.0	3.2310386496843275E-4
14	0.0	0.0	0.0	0.0	3.2310386496843275E-4
15	3.2310386496843275E-4	0.0	0.0	0.0	3.2310386496843275E-4
16	3.2310386496843275E-4	0.0	0.0	3.2310386496843275E-4	3.2310386496843275E-4
17	3.2310386496843275E-4	0.0	0.0	0.001292415459873731	3.2310386496843275E-4
18	6.462077299368655E-4	0.0	0.0	0.0016155193248421637	3.2310386496843275E-4
19	6.462077299368655E-4	0.0	0.0	0.0019386231898105965	3.2310386496843275E-4
20	6.462077299368655E-4	0.0	0.0	0.0019386231898105965	3.2310386496843275E-4
21	6.462077299368655E-4	0.0	0.0	0.003877246379621193	3.2310386496843275E-4
22	6.462077299368655E-4	0.0	0.0	0.008400700489179251	3.2310386496843275E-4
23	6.462077299368655E-4	0.0	0.0	0.010016219814021416	3.2310386496843275E-4
24	6.462077299368655E-4	0.0	0.0	0.01292415459873731	3.2310386496843275E-4
25	6.462077299368655E-4	0.0	0.0	0.015508985518484772	3.2310386496843275E-4
26	6.462077299368655E-4	0.0	0.0	0.020355543493011264	3.2310386496843275E-4
27	6.462077299368655E-4	0.0	0.0	0.04135729471595939	3.2310386496843275E-4
28	6.462077299368655E-4	3.2310386496843275E-4	0.0	0.13667293488164706	3.2310386496843275E-4
29	6.462077299368655E-4	3.2310386496843275E-4	0.0	0.2962862441760528	3.2310386496843275E-4
30	6.462077299368655E-4	3.2310386496843275E-4	0.0	0.504688237080692	3.2310386496843275E-4
31	6.462077299368655E-4	3.2310386496843275E-4	0.0	1.0536417036620591	3.2310386496843275E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	20	0.0018398582	37.0	13
TCTACGC	20	0.0018398582	37.0	10
TAAGCGG	30	8.292356E-6	37.0	18
CGCATTT	25	0.0054902155	29.6	14
TACGCAT	25	0.0054902155	29.6	12
CTACGCA	25	0.0054902155	29.6	11
TTACGAG	25	0.0054902155	29.6	6
TTTTACG	25	0.0054902155	29.6	4
GGTATCA	515	0.0	28.737864	1
TAGCGAA	55	1.8968398E-5	23.545454	10
GTAGCGA	55	1.8968398E-5	23.545454	9
AGCGAAA	55	1.8968398E-5	23.545454	11
TGTCGGG	55	1.8968398E-5	23.545454	23
GATCTCT	40	0.0019280165	23.125002	6
AGCGGAG	50	2.6959236E-4	22.2	20
AACGACC	50	2.6959236E-4	22.2	37
TATAAGA	50	2.6959236E-4	22.2	13
AATAAGC	50	2.6959236E-4	22.2	16
GGTAAGT	60	3.7149708E-5	21.583332	28
GCGAAAT	60	3.7149708E-5	21.583332	12
>>END_MODULE