FastQCFastQC Report
Fri 10 Feb 2017
ERR1631739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174946
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4030.2303567958112789No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3920.2240691413350405No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3240.18520000457283964No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3180.18177037485852776No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3100.17719753523944531No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2920.1669086460965098No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2850.16290741142981263No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2590.14804568266779464No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2560.14633086781063873No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2400.1371851885724738No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2230.12746790438192357No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2000.11432099047706148No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1980.11317778057229087No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1920.10974815085797902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAA200.001837673437.01
GGTATCA2450.032.469391
CTTATTG551.8368155E-830.27272828
TTATTGA659.296855E-825.61538529
GCAGTCG601.3266053E-624.6666669
CAGTCGG601.3266053E-624.66666610
CTCTATG851.9481377E-923.9411771
GCCTTAT551.8907287E-523.54545426
TTGGACA400.001924683523.1254
AGTCGGT652.6613143E-622.7692311
GTATCAA3500.022.7285732
TATGGGC903.772584E-922.6111134
TCTATGG903.772584E-922.6111132
TTAATAC502.689431E-422.1999992
CCTTATT603.703088E-521.58333227
TCCTCGC603.703088E-521.58333221
CTATGGG957.041308E-921.4210533
GGGCAGT705.0612343E-621.1428577
GTGATTC806.8909503E-720.812516
ATTCCTC806.8909503E-720.812519
Produced by FastQC (version 0.11.5)