##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174946
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54953528517371	34.0	31.0	34.0	31.0	34.0
2	32.71298572130829	34.0	31.0	34.0	31.0	34.0
3	32.80386519268803	34.0	31.0	34.0	31.0	34.0
4	36.259588673076266	37.0	37.0	37.0	35.0	37.0
5	36.16020372000503	37.0	35.0	37.0	35.0	37.0
6	36.207995610073965	37.0	36.0	37.0	35.0	37.0
7	36.201439301270106	37.0	36.0	37.0	35.0	37.0
8	36.17760337475564	37.0	36.0	37.0	35.0	37.0
9	38.00663633349719	39.0	38.0	39.0	35.0	39.0
10	37.91559109668126	39.0	38.0	39.0	35.0	39.0
11	38.0037611605867	39.0	38.0	39.0	35.0	39.0
12	37.943319652921474	39.0	38.0	39.0	35.0	39.0
13	37.992660592411376	39.0	38.0	39.0	35.0	39.0
14	39.36102568792656	40.0	39.0	41.0	36.0	41.0
15	39.36523841642564	40.0	39.0	41.0	36.0	41.0
16	39.31121031632618	40.0	39.0	41.0	36.0	41.0
17	39.27747990808592	40.0	39.0	41.0	36.0	41.0
18	39.29482811839082	40.0	39.0	41.0	36.0	41.0
19	39.314862871971926	40.0	39.0	41.0	36.0	41.0
20	39.3126278966081	40.0	39.0	41.0	36.0	41.0
21	39.269837549872534	40.0	39.0	41.0	36.0	41.0
22	39.237010277457046	40.0	39.0	41.0	36.0	41.0
23	39.173516399346084	40.0	39.0	41.0	36.0	41.0
24	39.19209356029861	40.0	39.0	41.0	36.0	41.0
25	39.13497879345627	40.0	39.0	41.0	36.0	41.0
26	39.07729242166154	40.0	39.0	41.0	36.0	41.0
27	38.951470739542486	40.0	39.0	41.0	35.0	41.0
28	38.950921998788196	40.0	38.0	41.0	35.0	41.0
29	38.90612531866976	40.0	38.0	41.0	35.0	41.0
30	38.872709293153314	40.0	38.0	41.0	35.0	41.0
31	38.78948932813554	40.0	38.0	41.0	35.0	41.0
32	38.73689595646657	40.0	38.0	41.0	35.0	41.0
33	38.67621437472134	40.0	38.0	41.0	35.0	41.0
34	38.65512786802785	40.0	38.0	41.0	35.0	41.0
35	38.581705211893954	40.0	38.0	41.0	35.0	41.0
36	38.54336766773747	40.0	38.0	41.0	35.0	41.0
37	38.4928835183428	40.0	38.0	41.0	34.0	41.0
38	38.45342562847964	40.0	38.0	41.0	34.0	41.0
39	38.38029449087147	40.0	38.0	41.0	34.0	41.0
40	38.06095023607285	40.0	37.0	41.0	34.0	41.0
41	38.15583094211928	40.0	37.0	41.0	34.0	41.0
42	38.1372880774639	40.0	37.0	41.0	34.0	41.0
43	37.657848707601204	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	4.0
19	8.0
20	9.0
21	15.0
22	27.0
23	67.0
24	97.0
25	166.0
26	246.0
27	396.0
28	586.0
29	874.0
30	1241.0
31	1732.0
32	2381.0
33	3308.0
34	4697.0
35	6904.0
36	10993.0
37	19244.0
38	43527.0
39	78422.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.441667714609075	16.615984360888504	12.838247230574007	28.104100693928412
2	20.861865947206567	19.52373875367256	32.4345798131995	27.17981548592137
3	20.812707921301428	19.962731357104477	29.41250442993838	29.812056291655708
4	15.72599545002458	15.743143598596138	33.60522675568461	34.92563419569467
5	16.780034982223086	32.913013158346004	33.668674905399385	16.638276954031532
6	34.69184777016908	33.47947366615984	15.344163341831193	16.48451522183988
7	30.634595818138166	27.569078458495767	20.063905433676677	21.73242028968939
8	27.771426611640166	30.775210636424955	20.130783213105758	21.322579538829125
9	28.576246384598676	12.608462039715112	17.51283253118105	41.30245904450516
10	19.12704491671716	23.73132280818081	30.002400740800017	27.13923153430201
11	39.14407874429824	19.356258502623668	18.817806637476707	22.681856115601384
12	23.093411681318806	23.783338858847873	26.533330284773587	26.589919175059734
13	34.97593543150458	17.67974117727756	20.804133847015652	26.540189544202207
14	24.250911709899054	19.937580739199525	22.875058589507617	32.9364489613938
15	29.58913036022544	24.287494426851712	19.84326592205595	26.2801092908669
16	25.88855989848296	23.979399357516034	22.274873389503046	27.857167354497957
17	26.9957586912533	23.62786231179907	21.771861031403976	27.60451796554365
18	26.668229053536518	21.555222754449947	23.522115395607788	28.254432796405748
19	28.164119213928867	22.218856104169287	23.35292032970174	26.264104352200107
20	29.803482217369933	20.92817212168326	22.823042538840557	26.44530312210625
21	27.3575846261132	22.342894378836895	22.1171104226447	28.182410572405196
22	28.471071073359777	22.779600562459272	21.28656842682885	27.4627599373521
23	27.711979696592092	22.19027585655002	22.59611537274359	27.501629074114298
24	27.921187109165114	21.462622752163522	23.000240074080004	27.61595006459136
25	27.94862414687961	21.967921530072136	22.963657357127342	27.119796965920912
26	27.87374389811713	22.668709201696522	22.44749808512341	27.01004881506293
27	26.983183382300822	21.676974609308015	24.0039783704686	27.335863637922557
28	26.185794473723323	22.62526722531524	22.404056108742125	28.784882192219314
29	27.218113017731188	23.474672184559807	22.63498450950579	26.67223028820322
30	26.774547574680184	22.18227338721663	23.642724040561085	27.400454997542102
31	27.870885873355206	22.461216603980656	22.85962525579322	26.80827226687092
32	25.750231500005714	22.360614132360844	23.003669703794312	28.88548466383913
33	26.064614223817635	21.841596835594984	24.746493203617113	27.347295736970267
34	26.545333988773677	21.91533387445269	23.877082071039062	27.66225006573457
35	25.849690761720762	23.292330204748893	24.41496233123364	26.443016702296706
36	26.141209287437267	22.33031906988442	24.733346289712255	26.79512535296606
37	26.60478090382175	21.852457329690306	24.424108010471805	27.118653756016144
38	25.695357424576727	21.383169663781967	25.866267305339935	27.055205606301374
39	25.15290432476307	20.405725195203093	26.556194482869	27.88517599716484
40	24.805368513712803	21.470053616544536	27.39531055297063	26.329267316772032
41	23.423799343797512	20.717249894253083	28.29730316783465	27.561647594114756
42	22.20742400512158	20.848719033301705	29.199867387651047	27.74398957392567
43	21.28428200701931	21.462051147211138	29.107838990317013	28.145827855452538
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	7.5
4	10.0
5	10.0
6	11.5
7	13.0
8	12.5
9	12.0
10	22.0
11	32.0
12	32.0
13	64.5
14	97.0
15	198.5
16	300.0
17	300.0
18	300.0
19	300.0
20	315.5
21	331.0
22	318.0
23	305.0
24	365.5
25	426.0
26	426.0
27	525.0
28	624.0
29	859.0
30	1094.0
31	1266.5
32	1439.0
33	1439.0
34	1808.0
35	2177.0
36	2500.5
37	2824.0
38	3621.0
39	4418.0
40	4418.0
41	5206.0
42	5994.0
43	6837.5
44	7681.0
45	10169.5
46	12658.0
47	12658.0
48	13901.0
49	15144.0
50	17133.0
51	19122.0
52	19785.5
53	20449.0
54	20449.0
55	18609.0
56	16769.0
57	15890.0
58	15011.0
59	14063.0
60	13115.0
61	13115.0
62	11893.5
63	10672.0
64	8991.0
65	7310.0
66	6395.5
67	5481.0
68	5481.0
69	4695.5
70	3910.0
71	3332.5
72	2755.0
73	2227.0
74	1699.0
75	1699.0
76	1394.0
77	1089.0
78	879.0
79	669.0
80	553.0
81	437.0
82	437.0
83	352.5
84	268.0
85	204.5
86	141.0
87	112.5
88	84.0
89	84.0
90	69.0
91	54.0
92	36.5
93	19.0
94	12.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	174946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.29293935525578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32713685808658	51.185896904393935
2	6.374322748788977	7.559046643495443
3	2.25802457479591	4.016547425281533
4	1.1806710929446664	2.8002183804988663
5	0.7457776301183175	2.2109673897555893
6	0.5218437087088952	1.856498842403897
7	0.3818855916372867	1.585018345791693
8	0.28491464124469734	1.3514741235797034
9	0.2159353069598403	1.1523095154205516
>10	1.5685300768980281	17.511666673384067
>50	0.0919724455542247	3.7776072744629894
>100	0.0489853242625762	4.992748481531737
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	403	0.2303567958112789	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	392	0.2240691413350405	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	324	0.18520000457283964	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	318	0.18177037485852776	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	310	0.17719753523944531	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	292	0.1669086460965098	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	285	0.16290741142981263	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	259	0.14804568266779464	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	256	0.14633086781063873	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	240	0.1371851885724738	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	223	0.12746790438192357	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	200	0.11432099047706148	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	198	0.11317778057229087	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	192	0.10974815085797902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	5.716049523853074E-4	0.0	0.0	0.0	0.0
11	5.716049523853074E-4	0.0	0.0	0.0	0.0
12	5.716049523853074E-4	0.0	0.0	0.0	0.0
13	5.716049523853074E-4	0.0	0.0	0.0	0.0
14	5.716049523853074E-4	0.0	0.0	0.0	0.0
15	5.716049523853074E-4	0.0	0.0	0.0	0.0
16	5.716049523853074E-4	0.0	0.0	5.716049523853074E-4	0.0
17	5.716049523853074E-4	0.0	0.0	0.0011432099047706148	0.0
18	5.716049523853074E-4	0.0	0.0	0.0028580247619265373	0.0
19	5.716049523853074E-4	0.0	0.0	0.0028580247619265373	0.0
20	5.716049523853074E-4	0.0	0.0	0.0040012346666971526	0.0
21	5.716049523853074E-4	0.0	0.0	0.008002469333394305	0.0
22	5.716049523853074E-4	0.0	0.0	0.013146913904862072	0.0
23	5.716049523853074E-4	0.0	0.0	0.01600493866678861	0.0
24	5.716049523853074E-4	0.0	0.0	0.018291358476329837	0.0
25	5.716049523853074E-4	0.0	0.0	0.022292593143026992	0.0
26	5.716049523853074E-4	0.0	0.0	0.02457901295256822	0.0
27	5.716049523853074E-4	0.0	0.0	0.04172716152412745	0.0
28	5.716049523853074E-4	0.0	0.0	0.1223234598104558	0.0
29	5.716049523853074E-4	0.0	0.0	0.28580247619265375	0.0
30	5.716049523853074E-4	0.0	0.0	0.4521395173367782	0.0
31	5.716049523853074E-4	0.0	0.0	0.9162827386736478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTAA	20	0.0018376734	37.0	1
GGTATCA	245	0.0	32.46939	1
CTTATTG	55	1.8368155E-8	30.272728	28
TTATTGA	65	9.296855E-8	25.615385	29
GCAGTCG	60	1.3266053E-6	24.666666	9
CAGTCGG	60	1.3266053E-6	24.666666	10
CTCTATG	85	1.9481377E-9	23.941177	1
GCCTTAT	55	1.8907287E-5	23.545454	26
TTGGACA	40	0.0019246835	23.125	4
AGTCGGT	65	2.6613143E-6	22.76923	11
GTATCAA	350	0.0	22.728573	2
TATGGGC	90	3.772584E-9	22.611113	4
TCTATGG	90	3.772584E-9	22.611113	2
TTAATAC	50	2.689431E-4	22.199999	2
CCTTATT	60	3.703088E-5	21.583332	27
TCCTCGC	60	3.703088E-5	21.583332	21
CTATGGG	95	7.041308E-9	21.421053	3
GGGCAGT	70	5.0612343E-6	21.142857	7
GTGATTC	80	6.8909503E-7	20.8125	16
ATTCCTC	80	6.8909503E-7	20.8125	19
>>END_MODULE