FastQCFastQC Report
Fri 10 Feb 2017
ERR1631738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346978
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10740.30952971081740055No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9730.28042123708131356No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9160.2639936825965911No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9040.2605352500734917No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8850.25505939857858423No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8310.23949645222463672No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8120.23402060072972927No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7680.22133968147836464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.21154078932958284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7140.20577673512441713No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7050.20318291073209255No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6890.19857166736795992No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6730.19396042400382732No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6440.185602545406337No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6440.185602545406337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5840.16831038279083976No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5210.15015361204456765No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5210.15015361204456765No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4940.1423721388675939No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4880.1406429226060442No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4870.1403547198957859No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4740.1366080846624282No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4650.1340142602701036No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4460.12853840877519612No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4280.12335075999054695No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4270.12306255728028867No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4270.12306255728028867No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4210.12133334101873892No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4100.11816311120589779No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3940.11355186784176517No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3920.11297546242124862No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3820.11009343531866575No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3810.10980523260840744No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3800.10951702989814915No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3750.10807601634685773No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3740.10778781363659945No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3730.10749961092634114No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3730.10749961092634114No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3710.10692320550582457No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3680.1060585973750497No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3650.10519398924427487No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3620.10432938111349999No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3610.10404117840324172No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3600.10375297569298343No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3530.1017355567211754No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3530.1017355567211754No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3490.10058274588014225No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3490.10058274588014225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCTT303.5943463E-430.8333325
GGATCCC250.00549112229.5999989
AGGATCC250.00549112229.5999988
GGTATCA8050.028.9565221
AATAGCC509.067226E-625.89999821
GTCGGTG2000.024.97499812
TAGCATC451.3206122E-424.6666685
CAGTCGG1950.024.66666810
GAATAGC601.3329154E-624.66666620
TGAATAG601.3329154E-624.66666619
AGTCGGT2000.024.0511
GCAGTCG2100.023.7857139
GGTAGCG701.9148865E-723.7857138
TCCTCGC1650.023.54545621
TCTAGCA551.8976993E-523.5454543
TTTCGGG400.001928485723.12522
TTTTTGC400.001928485723.1252
TGATTCC2100.022.9047617
GATTCCT2100.022.9047618
TAGCGAA652.6738908E-622.7692310
Produced by FastQC (version 0.11.5)