##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631738.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346978
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.583613370300135	34.0	31.0	34.0	31.0	34.0
2	32.74912530477437	34.0	31.0	34.0	31.0	34.0
3	32.835179752030385	34.0	31.0	34.0	31.0	34.0
4	36.26851846514764	37.0	37.0	37.0	35.0	37.0
5	36.190418412694754	37.0	35.0	37.0	35.0	37.0
6	36.24823475839967	37.0	37.0	37.0	35.0	37.0
7	36.24130060119085	37.0	37.0	37.0	35.0	37.0
8	36.22489322089585	37.0	37.0	37.0	35.0	37.0
9	38.05912190398239	39.0	38.0	39.0	35.0	39.0
10	37.985912651522575	39.0	38.0	39.0	35.0	39.0
11	38.07179417715244	39.0	38.0	39.0	37.0	39.0
12	37.990307742854014	39.0	38.0	39.0	35.0	39.0
13	38.05728028866383	39.0	38.0	39.0	35.0	39.0
14	39.410305552513414	40.0	39.0	41.0	37.0	41.0
15	39.4257964481898	40.0	39.0	41.0	37.0	41.0
16	39.354742952002724	40.0	39.0	41.0	36.0	41.0
17	39.340577788793524	40.0	39.0	41.0	36.0	41.0
18	39.373156223161125	40.0	39.0	41.0	37.0	41.0
19	39.396682210399504	40.0	39.0	41.0	37.0	41.0
20	39.395454466853806	40.0	39.0	41.0	37.0	41.0
21	39.356962112871706	40.0	39.0	41.0	36.0	41.0
22	39.32613595098248	40.0	39.0	41.0	36.0	41.0
23	39.27078085642317	40.0	39.0	41.0	36.0	41.0
24	39.28097458628501	40.0	39.0	41.0	36.0	41.0
25	39.221907440817574	40.0	39.0	41.0	36.0	41.0
26	39.16758411196099	40.0	39.0	41.0	36.0	41.0
27	39.02804212370813	40.0	39.0	41.0	35.0	41.0
28	39.039613462525004	40.0	39.0	41.0	35.0	41.0
29	39.015150816478275	40.0	39.0	41.0	35.0	41.0
30	38.97914853391281	40.0	38.0	41.0	35.0	41.0
31	38.8957830179435	40.0	38.0	41.0	35.0	41.0
32	38.83888315685721	40.0	38.0	41.0	35.0	41.0
33	38.77876695352443	40.0	38.0	41.0	35.0	41.0
34	38.757713745540066	40.0	38.0	41.0	35.0	41.0
35	38.67312048602505	40.0	38.0	41.0	35.0	41.0
36	38.63466271636818	40.0	38.0	41.0	35.0	41.0
37	38.586919055386794	40.0	38.0	41.0	35.0	41.0
38	38.55685663067975	40.0	38.0	41.0	35.0	41.0
39	38.46858878660895	40.0	38.0	41.0	34.0	41.0
40	38.127267434822954	40.0	38.0	41.0	34.0	41.0
41	38.20324631532835	40.0	37.0	41.0	34.0	41.0
42	38.185115482826	40.0	37.0	41.0	34.0	41.0
43	37.71239963340615	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	5.0
20	12.0
21	20.0
22	43.0
23	90.0
24	138.0
25	281.0
26	440.0
27	714.0
28	1139.0
29	1655.0
30	2328.0
31	3252.0
32	4508.0
33	6204.0
34	8797.0
35	13605.0
36	20228.0
37	37013.0
38	83620.0
39	162882.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.68455060551389	16.61229242199794	13.095354748716057	27.60780222377211
2	21.02669333502412	19.872729683149938	32.76720714281597	26.33336983900997
3	21.178864366040496	19.999250672953327	29.94137956873346	28.88050539227271
4	14.943886932312711	16.70278807301904	35.741747315391756	32.61157767927649
5	16.543411974246204	32.74876216935944	34.06094910916542	16.64687674722893
6	31.709791398878316	35.010865242176735	16.343111090616695	16.93623226832825
7	29.009043801047905	28.369810189695023	20.394952994137956	22.226193015119115
8	26.883837015603294	30.218918778712194	20.880574560923172	22.01666964476134
9	28.529474491178114	12.588982586792246	18.43776838877393	40.44377453325571
10	19.619111298122647	23.012121805993463	29.383995527093937	27.98477136878995
11	38.39292404705773	19.914230873427133	19.007256944244304	22.685588135270823
12	22.376058424453422	24.906478220521187	26.64318775253762	26.074275602487766
13	35.42587714494867	17.968862579183696	20.68574952878857	25.919510747079066
14	23.568929442212475	21.41115575050868	23.98855258834854	31.031362218930308
15	30.072800004611246	24.137265186841816	20.69266639383477	25.097268414712172
16	24.142741038336723	24.68513853904282	23.516188346235207	27.65593207638525
17	26.01144741165146	24.030053778625735	22.373176397350843	27.585322412371966
18	26.24690902593248	21.19183348800212	24.004691940123006	28.55656554594239
19	27.5933920882592	22.671754405178426	24.196346742444767	25.538506764117606
20	30.1843344534812	20.611105026831673	23.351624598677727	25.8529359210094
21	27.178380185487267	22.514107522667143	22.45906080500781	27.84845148683778
22	28.003216342246485	23.33548524690326	22.14809008063912	26.513208330211135
23	27.62912922433123	22.153277729423767	23.21299909504349	27.004593951201517
24	27.24956625492106	21.823862031598544	23.85713215247076	27.069439561009634
25	27.123909873248447	22.401708465666413	24.02918917049496	26.44519249059018
26	27.337468081549837	23.303782948774852	23.10232925430431	26.256419715371003
27	26.17572295649868	21.957011683737875	25.58029615710506	26.286969202658383
28	25.491817925055766	23.30580036774666	22.91038624927229	28.291995457925285
29	26.625895589922127	24.040429076195032	23.178126567102236	26.1555487667806
30	26.258725337053068	22.200831176616383	24.8704528817389	26.669990604591646
31	27.36110070379102	22.948140804316125	23.5680648340817	26.12269365781116
32	24.845667448656688	22.646392566675697	23.79862700228833	28.709312982379288
33	24.975935073693435	22.04866014560001	25.902794989884086	27.072609790822472
34	25.502481425335322	21.97228642738156	25.243098986102865	27.28213316118025
35	24.88716863893388	24.083947685444034	25.355786245813857	25.673097429808227
36	25.550899480658718	22.7838652594689	25.64744738859524	26.017787871277143
37	25.937667517825336	22.538892955749358	25.293246257687805	26.2301932687375
38	25.35031039431895	21.548628443301883	26.09934923828024	27.001711924098938
39	24.545360224567553	20.679985474583404	27.359083284819206	27.415571016029833
40	24.420280248315454	21.755269786557072	27.90609202888944	25.918357936238035
41	22.68472352714005	20.60620558075728	29.174183954025906	27.534886938076763
42	20.829274478497194	21.42614229144211	30.158396209557953	27.586187020502738
43	20.27448426124999	22.306601571281178	29.41512141980183	28.003792747667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	12.5
4	18.0
5	18.0
6	32.5
7	47.0
8	56.5
9	66.0
10	112.0
11	158.0
12	158.0
13	316.0
14	474.0
15	853.5
16	1233.0
17	1193.5
18	1154.0
19	1154.0
20	1288.5
21	1423.0
22	1075.0
23	727.0
24	714.0
25	701.0
26	701.0
27	775.0
28	849.0
29	1032.0
30	1215.0
31	1559.5
32	1904.0
33	1904.0
34	2705.5
35	3507.0
36	4085.5
37	4664.0
38	6072.5
39	7481.0
40	7481.0
41	9333.5
42	11186.0
43	13485.5
44	15785.0
45	22415.0
46	29045.0
47	29045.0
48	32696.0
49	36347.0
50	40978.5
51	45610.0
52	44964.0
53	44318.0
54	44318.0
55	39608.5
56	34899.0
57	32393.0
58	29887.0
59	27436.5
60	24986.0
61	24986.0
62	21924.5
63	18863.0
64	14759.5
65	10656.0
66	8925.0
67	7194.0
68	7194.0
69	5970.0
70	4746.0
71	3913.0
72	3080.0
73	2365.5
74	1651.0
75	1651.0
76	1355.5
77	1060.0
78	869.5
79	679.0
80	592.0
81	505.0
82	505.0
83	436.0
84	367.0
85	293.5
86	220.0
87	183.5
88	147.0
89	147.0
90	111.5
91	76.0
92	52.0
93	28.0
94	19.5
95	11.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	346978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.6049495665054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.84814460329112	35.7234597206827
2	6.753503164496815	5.754653234412428
3	2.626265611761016	3.3567574181197655
4	1.4268656817401006	2.431661616348573
5	0.9176127143053566	1.9547421707281927
6	0.6510173033076898	1.6641935624607886
7	0.4806704008991294	1.4335256731893535
8	0.3782058044474173	1.2890751379393453
9	0.27394906035610167	1.050442731023693
>10	2.1831894035029853	18.66279894803054
>50	0.26376613544491545	7.84250380652292
>100	0.1846362948114408	14.91968850696784
>500	0.011497498211701441	3.607027322998637
>1k	6.763234242177318E-4	0.3094701505752146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1074	0.30952971081740055	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	973	0.28042123708131356	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	916	0.2639936825965911	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	904	0.2605352500734917	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	885	0.25505939857858423	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	831	0.23949645222463672	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	812	0.23402060072972927	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	768	0.22133968147836464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.21154078932958284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.20577673512441713	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	705	0.20318291073209255	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	689	0.19857166736795992	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	673	0.19396042400382732	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	644	0.185602545406337	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	644	0.185602545406337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	584	0.16831038279083976	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	521	0.15015361204456765	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	521	0.15015361204456765	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	494	0.1423721388675939	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	488	0.1406429226060442	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	487	0.1403547198957859	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	474	0.1366080846624282	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	465	0.1340142602701036	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	446	0.12853840877519612	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	428	0.12335075999054695	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	427	0.12306255728028867	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	427	0.12306255728028867	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	421	0.12133334101873892	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	410	0.11816311120589779	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	394	0.11355186784176517	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	392	0.11297546242124862	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	382	0.11009343531866575	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	381	0.10980523260840744	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	380	0.10951702989814915	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	375	0.10807601634685773	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	374	0.10778781363659945	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	373	0.10749961092634114	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	373	0.10749961092634114	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	371	0.10692320550582457	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	368	0.1060585973750497	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	365	0.10519398924427487	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	362	0.10432938111349999	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	361	0.10404117840324172	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	360	0.10375297569298343	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	353	0.1017355567211754	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	353	0.1017355567211754	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	349	0.10058274588014225	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	349	0.10058274588014225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.882027102582873E-4	0.0
2	0.0	0.0	0.0	2.882027102582873E-4	0.0
3	0.0	0.0	0.0	2.882027102582873E-4	0.0
4	0.0	0.0	0.0	2.882027102582873E-4	0.0
5	0.0	0.0	0.0	2.882027102582873E-4	0.0
6	0.0	0.0	0.0	2.882027102582873E-4	0.0
7	0.0	0.0	0.0	2.882027102582873E-4	0.0
8	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
9	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
10	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
11	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
12	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
13	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
14	2.882027102582873E-4	0.0	0.0	2.882027102582873E-4	0.0
15	5.764054205165746E-4	0.0	0.0	2.882027102582873E-4	0.0
16	5.764054205165746E-4	0.0	0.0	0.0017292162615497237	0.0
17	5.764054205165746E-4	0.0	0.0	0.0025938243923245854	0.0
18	5.764054205165746E-4	0.0	0.0	0.0034584325230994474	0.0
19	5.764054205165746E-4	0.0	0.0	0.004323040653874309	0.0
20	5.764054205165746E-4	0.0	0.0	0.005475851494907458	0.0
21	5.764054205165746E-4	0.0	0.0	0.0072050677564571815	0.0
22	5.764054205165746E-4	0.0	0.0	0.010663500279556629	0.0
23	5.764054205165746E-4	0.0	0.0	0.01383373009239779	0.0
24	5.764054205165746E-4	0.0	0.0	0.016427554484722374	0.0
25	5.764054205165746E-4	0.0	0.0	0.018444973456530386	0.0
26	5.764054205165746E-4	0.0	0.0	0.026226446633504142	0.0
27	5.764054205165746E-4	0.0	0.0	0.05274109597726657	0.0
28	5.764054205165746E-4	0.0	0.0	0.15534126082921684	0.0
29	5.764054205165746E-4	0.0	0.0	0.34065560352529556	0.0
30	5.764054205165746E-4	0.0	0.0	0.5948503939731049	0.0
31	5.764054205165746E-4	0.0	0.0	1.259157641118457	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCCTT	30	3.5943463E-4	30.833332	5
GGATCCC	25	0.005491122	29.599998	9
AGGATCC	25	0.005491122	29.599998	8
GGTATCA	805	0.0	28.956522	1
AATAGCC	50	9.067226E-6	25.899998	21
GTCGGTG	200	0.0	24.974998	12
TAGCATC	45	1.3206122E-4	24.666668	5
CAGTCGG	195	0.0	24.666668	10
GAATAGC	60	1.3329154E-6	24.666666	20
TGAATAG	60	1.3329154E-6	24.666666	19
AGTCGGT	200	0.0	24.05	11
GCAGTCG	210	0.0	23.785713	9
GGTAGCG	70	1.9148865E-7	23.785713	8
TCCTCGC	165	0.0	23.545456	21
TCTAGCA	55	1.8976993E-5	23.545454	3
TTTCGGG	40	0.0019284857	23.125	22
TTTTTGC	40	0.0019284857	23.125	2
TGATTCC	210	0.0	22.90476	17
GATTCCT	210	0.0	22.90476	18
TAGCGAA	65	2.6738908E-6	22.76923	10
>>END_MODULE