FastQCFastQC Report
Fri 10 Feb 2017
ERR1631737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657211
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.21910771426528164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14380.21880339799546872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11570.17604696208675752No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10780.16402646942914834No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10320.15702719522345182No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9910.15078871169228755No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9490.14439807002621685No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8790.13374700058276567No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8590.13070383788463674No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8370.12735635891669495No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8060.12263945673459513No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7880.1199006103062791No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7410.11274917796567617No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6920.10529342935526034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6680.10164163411750564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA250.00549465329.5999984
ATACGGC405.9363178E-527.7529
GGTATCA10500.026.9571441
GTTTAGG701.9203253E-723.7857151
GGATCTT603.723162E-521.5833326
TGCGGGT3200.021.39062521
TATTAGA705.0964372E-621.1428592
TTCTGCG3150.021.14285918
TATTGCG450.00382409120.55555511
GTAACGT3150.020.55555526
GTACTAT555.1398744E-420.1818181
GTATCAA15150.018.6831682
TAAACCA701.2181892E-418.55
CGTCAAT3400.018.530
CCCCTAT500.00703175618.4999981
CTGCGGG3750.018.25333420
GGTAACG3650.017.73972525
ACGTCAA3650.017.73972529
AATGAGC3150.017.6190534
CGTGCCA3700.017.50000210
Produced by FastQC (version 0.11.5)