##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657211
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.453697518757295	33.0	31.0	34.0	31.0	34.0
2	32.62289736477326	34.0	31.0	34.0	31.0	34.0
3	32.7139092315862	34.0	31.0	34.0	31.0	34.0
4	36.199686249925826	37.0	35.0	37.0	35.0	37.0
5	36.090497572316956	37.0	35.0	37.0	35.0	37.0
6	36.14197571251851	37.0	36.0	37.0	35.0	37.0
7	36.13139767897981	37.0	35.0	37.0	35.0	37.0
8	36.10759710351775	37.0	35.0	37.0	35.0	37.0
9	37.90836580641529	39.0	38.0	39.0	35.0	39.0
10	37.82944594658336	39.0	38.0	39.0	35.0	39.0
11	37.907220055659444	39.0	38.0	39.0	35.0	39.0
12	37.845354079587835	39.0	38.0	39.0	35.0	39.0
13	37.89941738650144	39.0	38.0	39.0	35.0	39.0
14	39.24222053495757	40.0	39.0	41.0	36.0	41.0
15	39.240078148418085	40.0	39.0	41.0	36.0	41.0
16	39.18686388389726	40.0	39.0	41.0	36.0	41.0
17	39.15424270135466	40.0	39.0	41.0	36.0	41.0
18	39.175789814838765	40.0	39.0	41.0	36.0	41.0
19	39.20480789274677	40.0	39.0	41.0	36.0	41.0
20	39.191634041426575	40.0	39.0	41.0	36.0	41.0
21	39.15828097825508	40.0	39.0	41.0	36.0	41.0
22	39.12814149489281	40.0	39.0	41.0	36.0	41.0
23	39.06722194242032	40.0	39.0	41.0	36.0	41.0
24	39.07572453899889	40.0	39.0	41.0	36.0	41.0
25	39.02093695936313	40.0	39.0	41.0	36.0	41.0
26	38.94529002101304	40.0	38.0	41.0	35.0	41.0
27	38.84919607249422	40.0	38.0	41.0	35.0	41.0
28	38.83094622579354	40.0	38.0	41.0	35.0	41.0
29	38.794699114896126	40.0	38.0	41.0	35.0	41.0
30	38.744861239388875	40.0	38.0	41.0	35.0	41.0
31	38.66062497432331	40.0	38.0	41.0	35.0	41.0
32	38.613682668123325	40.0	38.0	41.0	35.0	41.0
33	38.55120805951209	40.0	38.0	41.0	35.0	41.0
34	38.51487117531508	40.0	38.0	41.0	35.0	41.0
35	38.43704837563583	40.0	38.0	41.0	34.0	41.0
36	38.39201869719162	40.0	38.0	41.0	34.0	41.0
37	38.34963809187612	40.0	38.0	41.0	34.0	41.0
38	38.30520183015805	40.0	38.0	41.0	34.0	41.0
39	38.23213701535732	40.0	38.0	41.0	34.0	41.0
40	37.905850632445286	40.0	37.0	41.0	33.0	41.0
41	37.98066222263474	40.0	37.0	41.0	33.0	41.0
42	37.9580378295555	40.0	37.0	41.0	33.0	41.0
43	37.4592178158917	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	3.0
18	6.0
19	12.0
20	26.0
21	68.0
22	129.0
23	262.0
24	379.0
25	676.0
26	1090.0
27	1751.0
28	2543.0
29	3657.0
30	5263.0
31	7180.0
32	9589.0
33	13009.0
34	18762.0
35	27779.0
36	42961.0
37	77667.0
38	177438.0
39	266954.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58830116963958	17.109269321420363	13.28462244241195	27.0178070665281
2	19.83944273604672	19.937280416791562	34.26114292061453	25.96213392654718
3	20.79651740460826	20.950957911538303	29.738090202385536	28.514434481467898
4	15.359450769996242	16.424709872476267	34.256578176567345	33.95926118096015
5	16.47674795461427	33.4483141639443	33.76525955895443	16.309678322486995
6	33.26968051356414	34.267533562280605	15.944498798711527	16.51828712544373
7	29.584714802399837	28.642247314789316	20.74858759211273	21.024450290698116
8	26.659626816958326	32.064588085105086	20.367431464172085	20.9083536337645
9	27.707539892059017	13.88990750306979	18.614874066319643	39.787678538551546
10	18.865022040105842	24.401600094946676	30.752528487806806	25.980849377140675
11	37.29654555386322	20.6108844800224	19.947779328100108	22.144790638014275
12	22.451237121715856	24.543411476679484	27.614419113496275	25.390932288108388
13	33.15738781000318	18.61472190818474	22.455193233223426	25.772697048588654
14	23.52821240058368	20.83349183139053	24.16468987889734	31.473605889128454
15	28.283458432679915	25.111874268689967	21.447297747603127	25.157369551026992
16	24.90569999589173	24.98649596552705	23.513453061497753	26.594350977083465
17	25.6155176952303	24.63774952032148	23.227395767873634	26.519337016574585
18	25.33326435497884	22.85689070937644	25.054814968100047	26.75502996754467
19	26.980528323476022	23.28034679882108	25.029861033975394	24.70926384372751
20	28.154428334279253	22.167462200115338	24.701960253252	24.976149212353416
21	26.015389273764438	23.617377067638856	23.62376770930493	26.743465949291778
22	26.7557907582192	23.953494387647194	23.22252670755663	26.068188146576976
23	26.336138622147224	23.59927024958499	24.18903518048237	25.87555594778542
24	26.70040519711326	23.12621060816085	24.190252445561622	25.983131749164272
25	26.590851339980613	23.18220480180642	24.598036247110898	25.628907611102065
26	26.422107968369367	23.825833712460685	24.00507599538048	25.746982323789467
27	26.093446396971444	23.072498786538876	24.870094992323622	25.96395982416606
28	25.26114139903319	23.90708615650073	24.052245017201475	26.77952742726461
29	26.049016221578764	24.295545874916883	24.05802702632792	25.59741087717643
30	25.55906702718001	23.635940360097443	25.050250224052856	25.754742388669698
31	26.312097636832004	23.448329379757794	24.429140717364742	25.810432266045456
32	24.272874312815823	23.54601490236773	24.725240447892684	27.45587033692376
33	24.794624557410025	23.068542675031306	25.934897620398928	26.201935147159737
34	25.188105494278094	22.8748453692954	25.626016606538844	26.31103252988766
35	24.96002045005333	23.93280088129992	25.660252186892794	25.446926481753955
36	25.044468214926408	22.97542189646856	26.14076757692735	25.839342311677683
37	25.296898560736203	22.352334334026665	26.00884647396346	26.34192063127367
38	24.69937356495859	21.956723183269908	26.970029412167477	26.373873839604023
39	24.17458015766626	21.28890112916552	27.93212529918093	26.60439341398729
40	23.82279054976256	22.138704312618017	28.337931044976422	25.700574092643002
41	22.33346672529827	21.66640546186841	29.443207736936845	26.55692007589648
42	21.47209952359288	22.08088422135357	29.592627025414973	26.85438922963858
43	20.937567995666537	22.5466402723022	29.544849371054347	26.970942360976917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	16.0
2	20.0
3	47.5
4	75.0
5	75.0
6	108.0
7	141.0
8	157.0
9	173.0
10	272.5
11	372.0
12	372.0
13	698.0
14	1024.0
15	1944.5
16	2865.0
17	2787.0
18	2709.0
19	2709.0
20	3082.5
21	3456.0
22	3029.0
23	2602.0
24	2803.0
25	3004.0
26	3004.0
27	3508.5
28	4013.0
29	5429.5
30	6846.0
31	7914.5
32	8983.0
33	8983.0
34	10811.0
35	12639.0
36	14250.5
37	15862.0
38	19159.5
39	22457.0
40	22457.0
41	25834.0
42	29211.0
43	32244.5
44	35278.0
45	42841.0
46	50404.0
47	50404.0
48	55395.0
49	60386.0
50	66609.5
51	72833.0
52	73103.5
53	73374.0
54	73374.0
55	65773.0
56	58172.0
57	53730.0
58	49288.0
59	44442.5
60	39597.0
61	39597.0
62	36142.0
63	32687.0
64	27069.0
65	21451.0
66	18379.5
67	15308.0
68	15308.0
69	13116.0
70	10924.0
71	9366.0
72	7808.0
73	6382.0
74	4956.0
75	4956.0
76	4041.0
77	3126.0
78	2555.0
79	1984.0
80	1607.5
81	1231.0
82	1231.0
83	1041.0
84	851.0
85	681.5
86	512.0
87	422.0
88	332.0
89	332.0
90	251.0
91	170.0
92	115.5
93	61.0
94	37.5
95	14.0
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	657211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.9663175093871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.29495226247003	43.11840984203981
2	6.410168251555981	6.405850042916779
3	2.194714931703842	3.289854693603209
4	1.1287777994032129	2.2560347969011274
5	0.6781806023314249	1.6943093652399681
6	0.47879626258850466	1.4354211647283066
7	0.3430960149458269	1.2000271093292028
8	0.263879748203013	1.0548079426407095
9	0.220809288073263	0.9929724297201344
>10	1.6393067519057485	16.781591897898718
>50	0.21274382661922628	7.395832585615649
>100	0.1278302198254153	11.58305139233181
>500	0.005211303925736327	1.8506046775032732
>1k	0.0015327364487459783	0.941232059531337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1440	0.21910771426528164	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1438	0.21880339799546872	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1157	0.17604696208675752	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1078	0.16402646942914834	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1032	0.15702719522345182	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	991	0.15078871169228755	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	949	0.14439807002621685	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	879	0.13374700058276567	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	859	0.13070383788463674	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	837	0.12735635891669495	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	806	0.12263945673459513	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	788	0.1199006103062791	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	741	0.11274917796567617	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	692	0.10529342935526034	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	668	0.10164163411750564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.5215813490644558E-4
11	0.0	0.0	0.0	0.0	1.5215813490644558E-4
12	0.0	0.0	0.0	0.0	1.5215813490644558E-4
13	0.0	0.0	0.0	0.0	1.5215813490644558E-4
14	0.0	0.0	0.0	4.564744047193367E-4	1.5215813490644558E-4
15	0.0	0.0	0.0	4.564744047193367E-4	1.5215813490644558E-4
16	0.0	0.0	0.0	9.129488094386734E-4	1.5215813490644558E-4
17	1.5215813490644558E-4	0.0	0.0	0.0015215813490644557	1.5215813490644558E-4
18	1.5215813490644558E-4	0.0	0.0	0.0016737394839709014	1.5215813490644558E-4
19	1.5215813490644558E-4	0.0	0.0	0.003499637102848248	1.5215813490644558E-4
20	1.5215813490644558E-4	0.0	0.0	0.004564744047193367	1.5215813490644558E-4
21	1.5215813490644558E-4	0.0	0.0	0.00791222301513517	1.5215813490644558E-4
22	1.5215813490644558E-4	0.0	0.0	0.010194595038731854	1.5215813490644558E-4
23	1.5215813490644558E-4	0.0	0.0	0.011716176387796309	1.5215813490644558E-4
24	1.5215813490644558E-4	0.0	0.0	0.014607180951018775	1.5215813490644558E-4
25	1.5215813490644558E-4	0.0	0.0	0.01719386924442835	1.5215813490644558E-4
26	1.5215813490644558E-4	0.0	0.0	0.021910771426528163	1.5215813490644558E-4
27	1.5215813490644558E-4	0.0	0.0	0.04397370098796277	1.5215813490644558E-4
28	3.0431626981289116E-4	0.0	0.0	0.14728907458943932	1.5215813490644558E-4
29	3.0431626981289116E-4	0.0	0.0	0.31222849282802634	1.5215813490644558E-4
30	3.0431626981289116E-4	0.0	0.0	0.5155117610630376	1.5215813490644558E-4
31	3.0431626981289116E-4	0.0	0.0	1.1621838344154312	1.5215813490644558E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	25	0.005494653	29.599998	4
ATACGGC	40	5.9363178E-5	27.75	29
GGTATCA	1050	0.0	26.957144	1
GTTTAGG	70	1.9203253E-7	23.785715	1
GGATCTT	60	3.723162E-5	21.583332	6
TGCGGGT	320	0.0	21.390625	21
TATTAGA	70	5.0964372E-6	21.142859	2
TTCTGCG	315	0.0	21.142859	18
TATTGCG	45	0.003824091	20.555555	11
GTAACGT	315	0.0	20.555555	26
GTACTAT	55	5.1398744E-4	20.181818	1
GTATCAA	1515	0.0	18.683168	2
TAAACCA	70	1.2181892E-4	18.5	5
CGTCAAT	340	0.0	18.5	30
CCCCTAT	50	0.007031756	18.499998	1
CTGCGGG	375	0.0	18.253334	20
GGTAACG	365	0.0	17.739725	25
ACGTCAA	365	0.0	17.739725	29
AATGAGC	315	0.0	17.61905	34
CGTGCCA	370	0.0	17.500002	10
>>END_MODULE