FastQCFastQC Report
Fri 10 Feb 2017
ERR1631736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787837
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18430.23393163814342308No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17980.2282197967346037No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17660.22415804284388777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16870.2141305879261827No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16150.20499164167207176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.20410313300847763No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13870.17605164520072047No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13240.16805506722837338No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12200.15485436708354647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1546005074653767No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11970.15193498147459436No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11880.1507926131928305No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11820.15003103433832124No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11570.1468577891111994No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10970.13924200056610694No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10620.13479945724813636No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9810.12451814271226154No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC8850.11233288104011362No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8710.11055586371292539No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8480.1076364781039733No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8060.10230542612240857No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8000.10154384726789932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAAC250.00549530829.59999817
TCGTATG250.00549530829.59999830
GGTATCA12450.028.9759041
CGTATGC400.00193065123.12531
TATTAGA652.6810685E-622.769232
ATACACA1005.329639E-1022.19999937
TCAGAAC855.1755705E-821.7647063
TATTAGC1403.6379788E-1219.821432
TTCTGCG5550.019.66666618
GTATCAA18100.019.5220992
CTTATAC7300.019.51369937
GTATTAG2100.019.380951
GCTACCG1355.638867E-1119.1851867
TGCGGGT5800.018.81896621
ATCTTAC609.233299E-418.51
GCCTAGT701.2185793E-418.51
TTATACA3050.018.19672237
GTAACGT5950.018.03361526
TTAGCTA1552.0008883E-1117.9032274
CTACCGT1552.0008883E-1117.9032278
Produced by FastQC (version 0.11.5)