FastQCFastQC Report
Fri 10 Feb 2017
ERR1631735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695083
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16450.2366623842044763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.22126853915287814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14240.2048676201259418No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT12710.18285586037926405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11860.1706271049644431No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11330.16300211629402533No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11240.16170730689716192No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10970.15782287870657175No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10360.14904694835005317No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10180.14645732955632637No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10140.14588185871327597No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9560.13753753148904518No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8770.12617198233879984No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8760.12602811462803723No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8760.12602811462803723No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7770.11178521126253987No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7520.10818851849347488No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT7480.10761304765042447No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7430.10689370909661149No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7250.10430409030288469No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT7160.1030092809060213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.030.0289841
ATACGGC509.084335E-625.90000229
ATCTAAC400.001930422823.12500220
GACTGAG400.001930422823.1250027
GTTCTAG502.7006125E-422.21
TCTATAC502.7006125E-422.23
TTCGTCC957.1322575E-921.4210534
GACAGGC3800.020.9342127
TCGGCGT450.003824308320.55555711
CCGTCAA450.003824308320.55555725
CTAACTT450.003824308320.55555722
TCTAAAC450.003824308320.5555573
AATCTAA450.003824308320.55555719
CTTATAC8100.020.55555537
GTATCAA15100.020.4602662
CCAGGAC4450.020.3707873
ACACCGT555.140284E-420.18181822
ACAGGCT4150.020.0602428
GTCTTCG1052.2555469E-819.380951
CGGCGTA500.00703215118.512
Produced by FastQC (version 0.11.5)