FastQCFastQC Report
Fri 10 Feb 2017
ERR1631734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628921
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10380.16504457634583677No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10080.16027450188497444No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8320.1322900650479154No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7890.1254529583206794No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7550.12004687393170207No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7190.11432278457866728No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7080.11257375727635108No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6900.10971171259983369No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6530.10382862076477013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6360.10112557857028148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAAC405.935919E-527.753
GGTATCA7900.027.6329141
TGTTACG659.377618E-825.61538516
AAACACG502.7002176E-422.19999915
GACCGTT806.949813E-720.81257
AGTATTA555.139536E-420.18181815
CGAACTA656.8957816E-519.92307724
GTACTAT759.254836E-619.7333341
CGTTATT951.6731065E-719.47368410
TATTAGA1052.2544555E-819.380952
CTTATAC7250.018.8827637
TACCTTA701.2180838E-418.531
AACGATT609.2304783E-418.522
ATAACGC801.6147746E-518.535
GTGTTAT500.007031427718.4999981
TTCGTCT500.007031427718.49999822
TTGTTAC1002.8697832E-718.49999815
ATCTAAG2150.018.06976732
TACCCTC1451.546141E-1017.862075
CCTCTAG1054.788726E-717.6190471