##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628921
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55868066100512	34.0	31.0	34.0	31.0	34.0
2	32.72976574164323	34.0	31.0	34.0	31.0	34.0
3	32.821146058089965	34.0	31.0	34.0	31.0	34.0
4	36.26813701561881	37.0	37.0	37.0	35.0	37.0
5	36.1708179564683	37.0	35.0	37.0	35.0	37.0
6	36.2199115628195	37.0	36.0	37.0	35.0	37.0
7	36.210725989432696	37.0	36.0	37.0	35.0	37.0
8	36.18620621667904	37.0	36.0	37.0	35.0	37.0
9	38.01122239518159	39.0	38.0	39.0	35.0	39.0
10	37.92411447542696	39.0	38.0	39.0	35.0	39.0
11	38.009586259641516	39.0	38.0	39.0	35.0	39.0
12	37.944884969654375	39.0	38.0	39.0	35.0	39.0
13	37.99872957016859	39.0	38.0	39.0	35.0	39.0
14	39.38453637261278	40.0	39.0	41.0	37.0	41.0
15	39.380927016270725	40.0	39.0	41.0	37.0	41.0
16	39.32826380419798	40.0	39.0	41.0	36.0	41.0
17	39.28815542810623	40.0	39.0	41.0	36.0	41.0
18	39.30731840724034	40.0	39.0	41.0	36.0	41.0
19	39.34126066707901	40.0	39.0	41.0	36.0	41.0
20	39.32151414883587	40.0	39.0	41.0	36.0	41.0
21	39.291192375512985	40.0	39.0	41.0	36.0	41.0
22	39.266591511493495	40.0	39.0	41.0	36.0	41.0
23	39.2005609607566	40.0	39.0	41.0	36.0	41.0
24	39.20925839652357	40.0	39.0	41.0	36.0	41.0
25	39.152292577287135	40.0	39.0	41.0	36.0	41.0
26	39.093137293873156	40.0	39.0	41.0	36.0	41.0
27	38.997195196217014	40.0	39.0	41.0	35.0	41.0
28	38.981174106127796	40.0	39.0	41.0	35.0	41.0
29	38.955126319521845	40.0	38.0	41.0	35.0	41.0
30	38.90888839774789	40.0	38.0	41.0	35.0	41.0
31	38.83475667055163	40.0	38.0	41.0	35.0	41.0
32	38.796034796103164	40.0	38.0	41.0	35.0	41.0
33	38.73708462589101	40.0	38.0	41.0	35.0	41.0
34	38.69831664072276	40.0	38.0	41.0	35.0	41.0
35	38.63374414274607	40.0	38.0	41.0	35.0	41.0
36	38.60350823076348	40.0	38.0	41.0	35.0	41.0
37	38.562191435808316	40.0	38.0	41.0	35.0	41.0
38	38.52279062076159	40.0	38.0	41.0	35.0	41.0
39	38.46506794971069	40.0	38.0	41.0	34.0	41.0
40	38.142485304195596	40.0	38.0	41.0	34.0	41.0
41	38.23006228127221	40.0	37.0	41.0	34.0	41.0
42	38.22575331400923	40.0	37.0	41.0	34.0	41.0
43	37.75588507936609	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	11.0
19	25.0
20	32.0
21	74.0
22	147.0
23	201.0
24	359.0
25	582.0
26	936.0
27	1455.0
28	2136.0
29	3119.0
30	4529.0
31	5941.0
32	8123.0
33	11284.0
34	16456.0
35	24552.0
36	37737.0
37	66673.0
38	156199.0
39	288347.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.86309409289879	16.604629198261787	12.712089435716093	27.82018727312333
2	20.27043142143449	19.308625407642612	33.352519632831466	27.06842353809143
3	20.64361024675595	20.3081150096753	29.250732603935948	29.7975421396328
4	15.722006420520223	15.514985188918798	33.67624868624199	35.086759704318986
5	16.789231079897156	33.02879057942095	33.848130369315065	16.333847971366833
6	35.05448220046715	33.61042165868209	15.168518780578163	16.166577360272594
7	31.023133271110364	27.655619704223582	20.207784443515166	21.11346258115089
8	27.609508984435248	31.596337218823983	19.963238626154954	20.83091517058581
9	28.412789523644467	13.161589452411352	17.7441999869618	40.681421036982385
10	18.857535366127067	23.851008314239785	30.777951443822037	26.513504875811112
11	38.7349126519865	19.790879935635793	19.118935446582324	22.35527196579539
12	23.263812148107633	23.799491510062474	26.777607998460855	26.159088343369042
13	33.56605996619607	17.96282839975132	21.83549285204342	26.635618782009185
14	24.347414063133527	19.619952267454895	23.34522141890635	32.68741225050523
15	28.810295728716323	24.42516627684558	20.545823720308274	26.218714274129816
16	26.247652725859048	24.051033436631947	22.284674863774622	27.41663897373438
17	26.631802722440494	23.90443314820144	22.29914408963924	27.164620039718816
18	26.290106388560723	22.2403131712886	24.020663962564456	27.448916477586216
19	27.92035883680144	22.3121822931656	23.635877956054895	26.13158091397807
20	28.951807937721906	21.484256369241926	23.391809146140773	26.172126546895395
21	27.074306629926493	22.78807672187763	22.38532343489882	27.752293213297058
22	28.017827358285064	23.224379532564505	21.77332288157018	26.984470227580253
23	27.306927261134543	22.863125893395196	22.984921794629216	26.84502505084104
24	27.497412234604983	22.46991275533811	22.921638806781775	27.111036203275134
25	27.566260309323425	22.417918943714714	23.326459126026958	26.689361620934903
26	27.370687256428074	23.008931169415554	22.814153128930343	26.806228445226033
27	27.219952903464822	22.170034074231896	23.43410380636042	27.175909215942863
28	26.148435177073114	22.801114925403986	23.01704029599902	28.03340960152388
29	27.074306629926493	23.17922282766834	23.01227022153816	26.734200320867007
30	26.553255496318297	22.827986344866844	23.819048815352005	26.79970934346285
31	27.138543632666106	22.72988181345511	23.198462127993817	26.93311242588497
32	25.297135888291216	22.731630840757425	23.477352481472234	28.493880789479125
33	25.807533855603488	22.344141792053374	24.823785499291645	27.024538853051496
34	26.142711087720077	22.01707368652025	24.587507810996932	27.252707414762746
35	25.890374148740463	23.304198778542933	24.770042660365927	26.035384412350677
36	25.836313304850687	22.470866770230284	25.087570616977327	26.605249307941698
37	26.071000968325116	22.021207751052994	24.914893921494112	26.992897359127777
38	25.473628643343122	21.540861252844156	25.901186317518416	27.084323786294306
39	24.932861201963362	20.78909751781225	26.809567497348635	27.46847378287575
40	24.614538232941815	21.694139645519865	27.462749693522714	26.2285724280156
41	23.235509706306516	20.60703967588934	28.643184120104117	27.514266497700028
42	22.34048473496671	21.066397846470384	29.078850920862877	27.514266497700028
43	21.691595605807407	21.243367608968374	29.297956341098484	27.76708044412573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	7.5
2	6.0
3	22.0
4	38.0
5	38.0
6	42.5
7	47.0
8	43.5
9	40.0
10	73.0
11	106.0
12	106.0
13	246.0
14	386.0
15	692.5
16	999.0
17	978.0
18	957.0
19	957.0
20	1171.5
21	1386.0
22	1263.0
23	1140.0
24	1408.0
25	1676.0
26	1676.0
27	2033.5
28	2391.0
29	3341.5
30	4292.0
31	5439.5
32	6587.0
33	6587.0
34	8194.5
35	9802.0
36	11396.0
37	12990.0
38	16089.5
39	19189.0
40	19189.0
41	21816.5
42	24444.0
43	27503.0
44	30562.0
45	37812.5
46	45063.0
47	45063.0
48	49210.0
49	53357.0
50	59952.0
51	66547.0
52	68595.5
53	70644.0
54	70644.0
55	65670.0
56	60696.0
57	57086.0
58	53476.0
59	48658.0
60	43840.0
61	43840.0
62	40193.5
63	36547.0
64	31105.0
65	25663.0
66	21925.0
67	18187.0
68	18187.0
69	15560.0
70	12933.0
71	11165.0
72	9397.0
73	7683.0
74	5969.0
75	5969.0
76	4801.5
77	3634.0
78	3029.5
79	2425.0
80	1932.5
81	1440.0
82	1440.0
83	1181.5
84	923.0
85	730.0
86	537.0
87	440.0
88	343.0
89	343.0
90	257.5
91	172.0
92	118.5
93	65.0
94	40.5
95	16.0
96	16.0
97	8.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	628921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.950891864827135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.74336184717284	45.93138373161219
2	6.321572366801254	6.69465789620345
3	2.0887479131767104	3.3180319465050996
4	1.1039620565272137	2.3382310191217863
5	0.6455612987576538	1.709152326131694
6	0.45409957760300873	1.4426986577712357
7	0.33594698658422967	1.2452084791255253
8	0.24631670246920068	1.0434151261557953
9	0.20341992763228595	0.969413993208732
>10	1.5543221573124049	16.582422220651967
>50	0.18963295671674907	7.015294623226804
>100	0.10883544410132966	10.094393212686539
>500	0.003617798695889037	1.2890773044524741
>1k	6.029664493148395E-4	0.32661946314671975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1038	0.16504457634583677	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1008	0.16027450188497444	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	832	0.1322900650479154	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	789	0.1254529583206794	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	755	0.12004687393170207	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	719	0.11432278457866728	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	708	0.11257375727635108	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	690	0.10971171259983369	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	653	0.10382862076477013	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	636	0.10112557857028148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.1800496405748894E-4	0.0	0.0	0.0	0.0
9	3.1800496405748894E-4	0.0	0.0	0.0	0.0
10	3.1800496405748894E-4	0.0	0.0	0.0	0.0
11	3.1800496405748894E-4	0.0	0.0	0.0	0.0
12	3.1800496405748894E-4	0.0	0.0	0.0	0.0
13	3.1800496405748894E-4	0.0	0.0	0.0	0.0
14	3.1800496405748894E-4	0.0	0.0	0.0	0.0
15	3.1800496405748894E-4	0.0	0.0	1.5900248202874447E-4	0.0
16	3.1800496405748894E-4	0.0	0.0	7.950124101437224E-4	0.0
17	3.1800496405748894E-4	0.0	0.0	0.0014310223382587002	0.0
18	3.1800496405748894E-4	0.0	0.0	0.0014310223382587002	1.5900248202874447E-4
19	3.1800496405748894E-4	0.0	0.0	0.0019080297843449337	1.5900248202874447E-4
20	3.1800496405748894E-4	0.0	0.0	0.0028620446765174005	1.5900248202874447E-4
21	3.1800496405748894E-4	0.0	0.0	0.007791121619408479	1.5900248202874447E-4
22	3.1800496405748894E-4	0.0	0.0	0.012561196080270813	1.5900248202874447E-4
23	3.1800496405748894E-4	0.0	0.0	0.015105235792730725	1.5900248202874447E-4
24	3.1800496405748894E-4	0.0	0.0	0.017967280469248125	1.5900248202874447E-4
25	3.1800496405748894E-4	0.0	0.0	0.01955730528953557	1.5900248202874447E-4
26	3.1800496405748894E-4	0.0	0.0	0.02210134500199548	1.5900248202874447E-4
27	4.7700744608623343E-4	0.0	0.0	0.041817652773559796	1.5900248202874447E-4
28	4.7700744608623343E-4	0.0	0.0	0.11543580195286848	1.5900248202874447E-4
29	4.7700744608623343E-4	0.0	0.0	0.24422781239615152	1.5900248202874447E-4
30	4.7700744608623343E-4	0.0	0.0	0.4218335848222591	1.5900248202874447E-4
31	4.7700744608623343E-4	0.0	0.0	0.9397046687898798	1.5900248202874447E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATAAC	40	5.935919E-5	27.75	3
GGTATCA	790	0.0	27.632914	1
TGTTACG	65	9.377618E-8	25.615385	16
AAACACG	50	2.7002176E-4	22.199999	15
GACCGTT	80	6.949813E-7	20.8125	7
AGTATTA	55	5.139536E-4	20.181818	15
CGAACTA	65	6.8957816E-5	19.923077	24
GTACTAT	75	9.254836E-6	19.733334	1
CGTTATT	95	1.6731065E-7	19.473684	10
TATTAGA	105	2.2544555E-8	19.38095	2
CTTATAC	725	0.0	18.88276	37
TACCTTA	70	1.2180838E-4	18.5	31
AACGATT	60	9.2304783E-4	18.5	22
ATAACGC	80	1.6147746E-5	18.5	35
GTGTTAT	50	0.0070314277	18.499998	1
TTCGTCT	50	0.0070314277	18.499998	22
TTGTTAC	100	2.8697832E-7	18.499998	15
ATCTAAG	215	0.0	18.069767	32
TACCCTC	145	1.546141E-10	17.86207	5
CCTCTAG	105	4.788726E-7	17.619047	1
>>END_MODULE