FastQCFastQC Report
Fri 10 Feb 2017
ERR1631733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987593
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.26873418503371327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24790.2510143348525152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20210.2046389555211509No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10340.10469900049919348No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9900.10024372388220654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.030.7572021
ATACGGC509.089397E-625.89999829
TAGGCTA652.6822108E-622.769235
AGACTAT450.003825431520.5555571
ACTAGAT450.003825431520.5555573
TCTAAGG555.142389E-420.1818183
TATACAC4200.019.82142837
GTATCAA19150.019.514362
CTTATAC14750.019.18982937
GCGGTAA3100.019.09677323
AGCATCG609.235563E-418.57
TAGAACT500.00703419918.4999984
TAGCTAG500.00703419918.4999985
ATACACA2800.017.83928537
TTAGGCA1054.7955655E-717.6190474
ACGGGTC1603.45608E-1117.3437515
TATGAAT1655.4569682E-1116.8181822
GTATAAT1107.804865E-716.8181821
TTCTAAG1005.876105E-616.652
GACAGGC3950.016.3924057
Produced by FastQC (version 0.11.5)