##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987593
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35274348846134	33.0	31.0	34.0	31.0	34.0
2	32.528864623382304	34.0	31.0	34.0	31.0	34.0
3	32.62326788464479	34.0	31.0	34.0	31.0	34.0
4	36.14961730186423	37.0	35.0	37.0	35.0	37.0
5	36.023519810286224	37.0	35.0	37.0	35.0	37.0
6	36.06000042527641	37.0	35.0	37.0	35.0	37.0
7	36.043186818861614	37.0	35.0	37.0	35.0	37.0
8	36.022506234855854	37.0	35.0	37.0	35.0	37.0
9	37.821160133779806	39.0	38.0	39.0	35.0	39.0
10	37.72153913606111	39.0	37.0	39.0	35.0	39.0
11	37.795179795725566	39.0	38.0	39.0	35.0	39.0
12	37.728010425347286	39.0	37.0	39.0	35.0	39.0
13	37.790306330644306	39.0	38.0	39.0	35.0	39.0
14	39.1221039436286	40.0	38.0	41.0	36.0	41.0
15	39.09737412071572	40.0	38.0	41.0	36.0	41.0
16	39.05630760849864	40.0	38.0	41.0	36.0	41.0
17	39.01418499321077	40.0	38.0	41.0	36.0	41.0
18	39.02294062432601	40.0	38.0	41.0	36.0	41.0
19	39.048097748768974	40.0	39.0	41.0	36.0	41.0
20	39.028776024131396	40.0	39.0	41.0	36.0	41.0
21	39.005236975150694	40.0	38.0	41.0	36.0	41.0
22	38.973099242299206	40.0	38.0	41.0	36.0	41.0
23	38.9224447722898	40.0	38.0	41.0	35.0	41.0
24	38.935967549385225	40.0	38.0	41.0	35.0	41.0
25	38.8711017595305	40.0	38.0	41.0	35.0	41.0
26	38.78797439836046	40.0	38.0	41.0	35.0	41.0
27	38.69624835331964	40.0	38.0	41.0	35.0	41.0
28	38.66923925139202	40.0	38.0	41.0	35.0	41.0
29	38.6233610404286	40.0	38.0	41.0	35.0	41.0
30	38.569107921988106	40.0	38.0	41.0	35.0	41.0
31	38.463881376234944	40.0	38.0	41.0	34.0	41.0
32	38.41300616752043	40.0	38.0	41.0	34.0	41.0
33	38.340557294351015	40.0	38.0	41.0	34.0	41.0
34	38.27681038646487	40.0	38.0	41.0	34.0	41.0
35	38.19671463852012	40.0	38.0	41.0	34.0	41.0
36	38.14804782941961	40.0	38.0	41.0	34.0	41.0
37	38.08757858753555	40.0	37.0	41.0	34.0	41.0
38	38.0545113219717	40.0	37.0	41.0	34.0	41.0
39	37.976308054026305	40.0	37.0	41.0	33.0	41.0
40	37.6308033775047	40.0	37.0	41.0	33.0	41.0
41	37.68645889551667	40.0	37.0	41.0	33.0	41.0
42	37.65180494393946	40.0	37.0	41.0	33.0	41.0
43	37.13972557521165	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	0.0
17	3.0
18	8.0
19	18.0
20	46.0
21	98.0
22	197.0
23	404.0
24	699.0
25	1184.0
26	1924.0
27	2897.0
28	4435.0
29	6356.0
30	8924.0
31	12191.0
32	16302.0
33	22180.0
34	31188.0
35	45906.0
36	71177.0
37	129311.0
38	277094.0
39	355046.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.49949827509916	17.28819463078414	12.549906692331762	26.662400401784947
2	19.941210599913124	19.49335404361918	34.5173568463932	26.048078510074497
3	21.133300863817382	20.575479980113265	28.742812069344353	29.548407086725
4	15.0810100922141	16.09600311059313	33.85301434902839	34.96997244816438
5	17.02189059663242	33.15313089501444	32.99274093680291	16.832237571550223
6	34.92795108916325	33.594102023809405	15.376172168089486	16.10177471893786
7	30.88316745865959	28.14469118351386	20.14109050995704	20.831050847869516
8	27.042313989669836	32.210029840227705	19.666907319108176	21.080748850994286
9	27.67202683696624	13.456150458741606	18.482411276710142	40.38941142758201
10	19.086810052319123	24.360338722530436	30.694932021591892	25.85791920355855
11	37.53904695557785	20.580137769303754	19.660224404182696	22.2205908709357
12	23.22201554689027	23.94913694203989	27.00160896239645	25.827238548673392
13	32.71884268114496	18.40444393591287	22.58126576433814	26.29544761860402
14	23.86681558091238	19.960854319542566	24.210378161854123	31.96195193769093
15	27.492803209419264	25.014150566073273	21.971905430678426	25.52114079382904
16	25.996944085265895	24.168863084286745	23.014946440487126	26.819246389960238
17	25.681935777187565	24.449950536303923	23.318917813309735	26.549195873198776
18	25.459678227771963	22.48081952788244	25.02437745103499	27.035124793310604
19	27.64104241322083	23.19690398777634	24.41066309704504	24.75139050195779
20	27.71718714085661	22.557673049525462	24.298167362466117	25.42697244715181
21	26.376452648003784	23.53347988493236	23.74743441883448	26.34263304822938
22	27.33767857811872	23.51474747188366	23.18627207766762	25.96130187233
23	25.945303379023542	23.523556768830886	24.094540969812464	26.436598882333108
24	26.53127351044408	23.509785913832925	23.601017828194408	26.35792274752859
25	26.372604909107295	23.09433136929889	24.692560599356213	25.8405031222376
26	26.841624029331925	23.629369588484327	23.61053591914888	25.918470463034875
27	26.823397897716973	23.079244182573184	23.884737943667076	26.21261997604276
28	25.568731248601395	23.656405017046495	24.243792736481527	26.531070997870582
29	26.537956425369558	23.994094733356757	24.36995806977166	25.097990771502026
30	26.181230527150355	22.662473306311405	24.6452739134441	26.51102225309414
31	25.725577236776687	22.971406237184752	24.532373153718183	26.77064337232038
32	24.506552800597007	23.270821077103623	24.702179946597436	27.520446175701935
33	25.330778974739594	22.743073310564167	25.609537532161532	26.316610182534706
34	25.93284885575333	22.812028841840718	25.58877999337784	25.66634230902811
35	25.834022719885617	23.271226102250623	24.841508597165028	26.053242580698726
36	25.23296540173938	22.477478070419696	25.049387753862167	27.240168773978755
37	24.45774727038365	22.09290669334432	26.56418180363773	26.885164232634295
38	24.692256830495964	21.26108629769551	26.627568239143045	27.419088632665478
39	23.533176116072106	21.554425760409398	28.29900576452041	26.61339235899809
40	24.469594255933366	21.52739033184723	28.020956001105716	25.98205941111369
41	22.617616771281288	21.537009679088452	29.016305299855304	26.829068249774956
42	21.505113948762293	22.718468032883994	28.80933744973891	26.967080568614804
43	21.64221496102139	21.814654417356138	28.735116591551378	27.808014030071092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	71.5
2	63.0
3	135.5
4	208.0
5	208.0
6	264.5
7	321.0
8	368.5
9	416.0
10	583.0
11	750.0
12	750.0
13	1370.5
14	1991.0
15	3489.5
16	4988.0
17	4813.0
18	4638.0
19	4638.0
20	5205.0
21	5772.0
22	5008.5
23	4245.0
24	4532.0
25	4819.0
26	4819.0
27	5372.5
28	5926.0
29	7280.0
30	8634.0
31	10769.0
32	12904.0
33	12904.0
34	18121.0
35	23338.0
36	25941.0
37	28544.0
38	32552.5
39	36561.0
40	36561.0
41	40256.5
42	43952.0
43	50944.0
44	57936.0
45	63187.0
46	68438.0
47	68438.0
48	71987.0
49	75536.0
50	83134.0
51	90732.0
52	93404.0
53	96076.0
54	96076.0
55	90989.5
56	85903.0
57	76965.5
58	68028.0
59	65330.0
60	62632.0
61	62632.0
62	59378.5
63	56125.0
64	44916.0
65	33707.0
66	30455.5
67	27204.0
68	27204.0
69	24424.5
70	21645.0
71	21423.5
72	21202.0
73	19633.0
74	18064.0
75	18064.0
76	13275.0
77	8486.0
78	6141.5
79	3797.0
80	2753.5
81	1710.0
82	1710.0
83	1368.0
84	1026.0
85	814.0
86	602.0
87	473.5
88	345.0
89	345.0
90	268.5
91	192.0
92	122.0
93	52.0
94	27.5
95	3.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	987593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.599397771320085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.89849125508279	39.1691947069678
2	6.567323420614509	5.989319858990148
3	2.1663139491376713	2.9634783439286383
4	1.1832447512308009	2.1582099228879987
5	0.7168849007880881	1.6344759873644685
6	0.49757390011126457	1.3613442115080385
7	0.36541515880206954	1.1663897824519966
8	0.2928376050339587	1.0682574747475366
9	0.2230448537748598	0.9153639907313182
>10	1.6722121172637425	15.145686757038785
>50	0.219847886647783	7.0882141613096366
>100	0.18428591596387867	16.848160293184783
>500	0.011629693723675914	3.656874900779549
>1k	8.945918248981472E-4	0.8350296081092907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2654	0.26873418503371327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2479	0.2510143348525152	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.2046389555211509	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1034	0.10469900049919348	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	990	0.10024372388220654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0125628674970357E-4	0.0	0.0	0.0	0.0
8	2.0251257349940713E-4	0.0	0.0	0.0	0.0
9	2.0251257349940713E-4	0.0	0.0	1.0125628674970357E-4	0.0
10	2.0251257349940713E-4	0.0	0.0	1.0125628674970357E-4	0.0
11	2.0251257349940713E-4	0.0	0.0	1.0125628674970357E-4	0.0
12	2.0251257349940713E-4	0.0	0.0	1.0125628674970357E-4	0.0
13	2.0251257349940713E-4	0.0	0.0	1.0125628674970357E-4	0.0
14	2.0251257349940713E-4	0.0	0.0	3.037688602491107E-4	0.0
15	2.0251257349940713E-4	0.0	0.0	5.062814337485178E-4	0.0
16	2.0251257349940713E-4	0.0	0.0	0.0012150754409964428	0.0
17	2.0251257349940713E-4	0.0	0.0	0.0018226131614946643	0.0
18	2.0251257349940713E-4	0.0	0.0	0.002328894595243182	0.0
19	2.0251257349940713E-4	0.0	0.0	0.002531407168742589	0.0
20	2.0251257349940713E-4	0.0	0.0	0.003341457462740218	0.0
21	2.0251257349940713E-4	0.0	0.0	0.004961558050735475	0.0
22	3.037688602491107E-4	0.0	0.0	0.006277889778481621	0.0
23	3.037688602491107E-4	0.0	0.0	0.007897990366476879	0.0
24	3.037688602491107E-4	0.0	0.0	0.010024372388220654	0.0
25	3.037688602491107E-4	0.0	0.0	0.0113407041159668	0.0
26	3.037688602491107E-4	0.0	0.0	0.016808543600450792	0.0
27	3.037688602491107E-4	0.0	0.0	0.049413067933855345	0.0
28	3.037688602491107E-4	0.0	0.0	0.1844889544579599	0.0
29	3.037688602491107E-4	0.0	0.0	0.37555956755465053	0.0
30	3.037688602491107E-4	0.0	0.0	0.6203972689154338	0.0
31	3.037688602491107E-4	0.0	0.0	1.3795156506779616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1215	0.0	30.757202	1
ATACGGC	50	9.089397E-6	25.899998	29
TAGGCTA	65	2.6822108E-6	22.76923	5
AGACTAT	45	0.0038254315	20.555557	1
ACTAGAT	45	0.0038254315	20.555557	3
TCTAAGG	55	5.142389E-4	20.181818	3
TATACAC	420	0.0	19.821428	37
GTATCAA	1915	0.0	19.51436	2
CTTATAC	1475	0.0	19.189829	37
GCGGTAA	310	0.0	19.096773	23
AGCATCG	60	9.235563E-4	18.5	7
TAGAACT	50	0.007034199	18.499998	4
TAGCTAG	50	0.007034199	18.499998	5
ATACACA	280	0.0	17.839285	37
TTAGGCA	105	4.7955655E-7	17.619047	4
ACGGGTC	160	3.45608E-11	17.34375	15
TATGAAT	165	5.4569682E-11	16.818182	2
GTATAAT	110	7.804865E-7	16.818182	1
TTCTAAG	100	5.876105E-6	16.65	2
GACAGGC	395	0.0	16.392405	7
>>END_MODULE