FastQCFastQC Report
Fri 10 Feb 2017
ERR1631732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229604
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5700.24825351474712984No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT4430.19294088953154126No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3880.16898660302085328No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3850.16768000557481577No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3850.16768000557481577No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3770.1641957457187157No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3760.1637602132367032No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2810.1223846274455149No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2760.12020696503545235No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2710.1180293026253898No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2620.11410951028727723No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG2620.11410951028727723No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2550.11106078291318966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11062525043117717No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2540.11062525043117717No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC2530.11018971794916466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2440.10626992561105207No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT2420.10539886064702705No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2400.10452779568300205No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2350.1023501332729395No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT2330.10147906830891447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC303.590592E-430.83333229
GGTATCA2750.030.2727281
AACTTAC250.00548730129.5999987
CTAAGTC250.00548730129.59999834
TAAGTCT250.00548730129.59999835
GATCCAC405.9197628E-527.756
TAAAGCC358.847703E-426.42857224
CAGATCC358.847703E-426.42857236
AGGGGTG358.847703E-426.4285725
ATAACGC358.847703E-426.42857235
GCGGTAA1750.023.25714123
TACGGCT400.001926509423.12530
CGCCTTA400.001926509423.12525
CAAGAGC400.001926509423.1254
GGCTAAG400.001926509423.1251
CCGCGGT1750.022.19999921
CGCGGTA1850.022.022
TAATACG1800.021.58333427
ACGGCTG603.7095953E-521.58333231
AGCCGCG1900.021.42105319
Produced by FastQC (version 0.11.5)