##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631732.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229604
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75905036497622	31.0	31.0	34.0	30.0	34.0
2	31.954848347589763	33.0	31.0	34.0	30.0	34.0
3	32.036401804846605	33.0	31.0	34.0	30.0	34.0
4	35.75898503510392	37.0	35.0	37.0	35.0	37.0
5	35.37644814550269	37.0	35.0	37.0	33.0	37.0
6	35.37190989704012	37.0	35.0	37.0	33.0	37.0
7	35.38255866622533	37.0	35.0	37.0	33.0	37.0
8	35.338866047629836	37.0	35.0	37.0	32.0	37.0
9	37.06304332677131	39.0	37.0	39.0	33.0	39.0
10	36.885546419051934	38.0	37.0	39.0	33.0	39.0
11	36.97172087594293	39.0	37.0	39.0	33.0	39.0
12	36.902614937022	38.0	37.0	39.0	33.0	39.0
13	36.980588317276705	39.0	37.0	39.0	33.0	39.0
14	38.109601749098445	40.0	38.0	40.0	33.0	41.0
15	38.078256476368004	40.0	37.0	40.0	33.0	41.0
16	38.007090468807164	39.0	37.0	40.0	33.0	41.0
17	37.95432135328653	39.0	37.0	40.0	33.0	41.0
18	37.94122489155241	39.0	37.0	40.0	33.0	41.0
19	37.95864183550809	40.0	37.0	40.0	33.0	41.0
20	37.93279298270065	39.0	37.0	40.0	33.0	41.0
21	37.90743192627306	39.0	37.0	40.0	33.0	41.0
22	37.85932736363478	39.0	37.0	40.0	33.0	41.0
23	37.800421595442586	39.0	37.0	40.0	33.0	41.0
24	37.77182017734883	39.0	37.0	40.0	33.0	41.0
25	37.71280117071131	39.0	37.0	40.0	33.0	41.0
26	37.562072089336425	39.0	37.0	40.0	33.0	41.0
27	37.45162976254769	39.0	37.0	40.0	32.0	41.0
28	37.42333757251616	39.0	37.0	40.0	32.0	41.0
29	37.37584275535269	39.0	36.0	40.0	32.0	41.0
30	37.30472465636487	39.0	36.0	40.0	32.0	41.0
31	37.24988676155468	39.0	36.0	40.0	32.0	41.0
32	37.20122907266424	39.0	36.0	40.0	32.0	41.0
33	37.11261127854915	39.0	36.0	40.0	31.0	41.0
34	37.05599641121235	39.0	36.0	40.0	31.0	41.0
35	36.95021428198115	39.0	36.0	40.0	31.0	41.0
36	36.90233619623351	39.0	35.0	40.0	31.0	41.0
37	36.84901830978554	39.0	35.0	40.0	31.0	41.0
38	36.83145328478598	39.0	35.0	40.0	31.0	41.0
39	36.769320220902074	39.0	35.0	40.0	31.0	41.0
40	36.382327834009864	38.0	35.0	40.0	30.0	41.0
41	36.463502378007355	38.0	35.0	40.0	30.0	41.0
42	36.42445253567011	38.0	35.0	40.0	30.0	41.0
43	35.86532029058727	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	12.0
20	27.0
21	54.0
22	97.0
23	197.0
24	336.0
25	538.0
26	778.0
27	1241.0
28	1763.0
29	2598.0
30	3516.0
31	4661.0
32	6306.0
33	9148.0
34	12968.0
35	18899.0
36	29161.0
37	46471.0
38	61190.0
39	29636.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.93043675197296	16.53107088726677	12.666591174369785	27.87190118639048
2	21.389871256598315	18.95829340952248	32.78644971341962	26.865385620459577
3	21.98742182191948	20.13684430584833	28.661086043797145	29.21464782843504
4	16.269751398059267	15.57769028414139	33.47241337258933	34.68014494521001
5	17.29281719830665	32.9249490426996	32.73810560791624	17.044128151077505
6	35.20975244333722	32.880089197052314	15.112541593352033	16.79761676625843
7	31.402763018065887	26.93419975261755	19.871605024302713	21.791432205013848
8	27.815281963728854	30.825682479399312	19.68519712200136	21.673838434870476
9	28.58225466455288	12.847772686886987	17.489242347694294	41.08073030086584
10	20.004442431316527	23.30534311248933	29.712025922893332	26.978188533300813
11	38.73625895019251	19.666904757756836	19.012734969774044	22.584101322276613
12	24.00306614867337	23.15421334123099	26.363652201181164	26.479068308914478
13	33.918834166652154	17.625128482082193	21.310168812390028	27.14586853887563
14	23.978240797198655	19.620302782181493	23.392885141373842	33.008571279246006
15	29.507761188829463	23.563178341840736	20.441717043257086	26.48734342607272
16	25.922893329384504	23.724325360185365	21.972613717531054	28.38016759289908
17	26.591000156791694	23.89548962561628	22.13985819062386	27.37365202696817
18	26.364958798627203	21.46826710336057	23.601505200257836	28.565268897754393
19	28.075730387972335	22.128969878573542	23.21518788871274	26.58011184474138
20	28.329210292503614	21.27227748645494	23.455166286301633	26.943345934739817
21	28.441577672862845	21.951708158394453	22.056235954077454	27.550478214665247
22	28.530426299193394	22.976951621051896	21.41469660807303	27.07792547168168
23	28.408912736711905	22.4129370568457	21.844567167819378	27.333583038623022
24	28.260831692827654	21.832372258323026	22.181233776415045	27.725562272434278
25	27.811797703872752	21.844131635337362	23.170328043065453	27.17374261772443
26	28.240361666173065	22.515287190118638	22.900733436699706	26.343617707008587
27	28.013449243044548	21.531419313252382	23.228253863173116	27.22687758052996
28	26.27306144492256	22.457361370010975	23.030086583857422	28.239490601209038
29	27.73470845455654	22.630703297851955	22.911621748750022	26.722966498841483
30	26.79395829340952	21.688646539258897	24.26395010539886	27.25344506193272
31	27.453790003658472	22.28968136443616	23.009616557202836	27.246912074702536
32	25.855821327154576	22.208672322781833	23.288792878172853	28.646713471890735
33	26.535251999094093	22.3571888991481	24.017003188097767	27.09055591366004
34	26.775230396682986	22.161199282242467	23.85890489712723	27.204665423947315
35	26.58838696189962	23.20473510914444	24.304454626226025	25.902423302729915
36	26.726886291179596	22.20431699796171	23.949060120903816	27.11973658995488
37	26.189874740858173	21.980888834689292	24.4220483963694	27.407188028083134
38	26.31095277085765	21.116792390376474	25.317067646905105	27.25518719186077
39	25.033100468632952	20.851117576348845	26.22733053431125	27.888451420706957
40	25.304437204926742	21.068012752391073	27.32792111635686	26.299628926325326
41	23.64941377327921	20.583700632393164	27.847511367397782	27.919374226929843
42	22.39420916011916	21.28577899339733	28.37363460566889	27.94637724081462
43	22.145084580408007	21.010522464765423	28.464225361927493	28.38016759289908
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	10.5
4	17.0
5	17.0
6	20.0
7	23.0
8	27.0
9	31.0
10	45.0
11	59.0
12	59.0
13	101.5
14	144.0
15	278.5
16	413.0
17	377.0
18	341.0
19	341.0
20	432.5
21	524.0
22	429.0
23	334.0
24	354.5
25	375.0
26	375.0
27	465.0
28	555.0
29	783.0
30	1011.0
31	1293.0
32	1575.0
33	1575.0
34	2369.0
35	3163.0
36	3661.0
37	4159.0
38	5286.5
39	6414.0
40	6414.0
41	7439.0
42	8464.0
43	10074.0
44	11684.0
45	14188.5
46	16693.0
47	16693.0
48	17919.5
49	19146.0
50	21624.0
51	24102.0
52	24557.0
53	25012.0
54	25012.0
55	23759.5
56	22507.0
57	20577.5
58	18648.0
59	17067.5
60	15487.0
61	15487.0
62	14901.0
63	14315.0
64	11482.5
65	8650.0
66	7655.5
67	6661.0
68	6661.0
69	5741.0
70	4821.0
71	4476.5
72	4132.0
73	4455.5
74	4779.0
75	4779.0
76	4102.5
77	3426.0
78	2301.5
79	1177.0
80	772.0
81	367.0
82	367.0
83	289.0
84	211.0
85	152.5
86	94.0
87	74.0
88	54.0
89	54.0
90	36.0
91	18.0
92	13.0
93	8.0
94	6.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	229604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.5217352520632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.96696281943066	38.59767078953325
2	7.263931313288811	6.758613788919877
3	2.700195014350317	3.768532727596393
4	1.5860833134348595	2.951493919813269
5	1.0373226914980842	2.412902581241575
6	0.7068144038184282	1.9729339540071487
7	0.48817677977156637	1.5897581633316302
8	0.37336988701028917	1.389585202766834
9	0.3354338437356468	1.404446802556653
>10	2.129405848031666	19.574012221051824
>50	0.27753156369260984	9.125188439875693
>100	0.13377420695974718	10.190134342391723
>500	9.98314977311546E-4	0.2647270669141462
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	570	0.24825351474712984	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	443	0.19294088953154126	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	388	0.16898660302085328	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	385	0.16768000557481577	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	385	0.16768000557481577	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	377	0.1641957457187157	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	376	0.1637602132367032	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	281	0.1223846274455149	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	276	0.12020696503545235	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	271	0.1180293026253898	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	262	0.11410951028727723	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	262	0.11410951028727723	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	255	0.11106078291318966	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.11062525043117717	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	254	0.11062525043117717	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	253	0.11018971794916466	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.10626992561105207	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	242	0.10539886064702705	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	240	0.10452779568300205	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	235	0.1023501332729395	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	233	0.10147906830891447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
9	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
10	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
11	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
12	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
13	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
14	4.355324820125085E-4	0.0	0.0	0.0	4.355324820125085E-4
15	4.355324820125085E-4	0.0	0.0	4.355324820125085E-4	4.355324820125085E-4
16	4.355324820125085E-4	0.0	0.0	0.0013065974460375255	0.0013065974460375255
17	4.355324820125085E-4	0.0	0.0	0.001742129928050034	0.0013065974460375255
18	4.355324820125085E-4	0.0	0.0	0.001742129928050034	0.001742129928050034
19	4.355324820125085E-4	0.0	0.0	0.001742129928050034	0.001742129928050034
20	4.355324820125085E-4	0.0	0.0	0.0021776624100625424	0.001742129928050034
21	4.355324820125085E-4	0.0	0.0	0.0030487273740875593	0.001742129928050034
22	4.355324820125085E-4	0.0	0.0	0.0030487273740875593	0.001742129928050034
23	4.355324820125085E-4	0.0	0.0	0.0030487273740875593	0.001742129928050034
24	4.355324820125085E-4	0.0	0.0	0.0047908573021375935	0.001742129928050034
25	4.355324820125085E-4	0.0	0.0	0.006968519712200136	0.001742129928050034
26	4.355324820125085E-4	0.0	0.0	0.012194909496350237	0.001742129928050034
27	4.355324820125085E-4	0.0	0.0	0.03702026097106322	0.001742129928050034
28	4.355324820125085E-4	0.0	0.0	0.13022421212174004	0.001742129928050034
29	4.355324820125085E-4	0.0	0.0	0.24956011219316737	0.001742129928050034
30	4.355324820125085E-4	0.0	0.0	0.41636905280395814	0.001742129928050034
31	4.355324820125085E-4	0.0	0.0	0.8688873016149544	0.001742129928050034
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	30	3.590592E-4	30.833332	29
GGTATCA	275	0.0	30.272728	1
AACTTAC	25	0.005487301	29.599998	7
CTAAGTC	25	0.005487301	29.599998	34
TAAGTCT	25	0.005487301	29.599998	35
GATCCAC	40	5.9197628E-5	27.75	6
TAAAGCC	35	8.847703E-4	26.428572	24
CAGATCC	35	8.847703E-4	26.428572	36
AGGGGTG	35	8.847703E-4	26.428572	5
ATAACGC	35	8.847703E-4	26.428572	35
GCGGTAA	175	0.0	23.257141	23
TACGGCT	40	0.0019265094	23.125	30
CGCCTTA	40	0.0019265094	23.125	25
CAAGAGC	40	0.0019265094	23.125	4
GGCTAAG	40	0.0019265094	23.125	1
CCGCGGT	175	0.0	22.199999	21
CGCGGTA	185	0.0	22.0	22
TAATACG	180	0.0	21.583334	27
ACGGCTG	60	3.7095953E-5	21.583332	31
AGCCGCG	190	0.0	21.421053	19
>>END_MODULE