FastQCFastQC Report
Fri 10 Feb 2017
ERR1631731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569866
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77830.49577479861338486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.4949467024574072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60800.38729420218031346No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA23900.15224229329127453No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23670.15077720009223716No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21840.13912015420424417No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG21460.13669956544061723No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20690.13179468820905732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.127909006246393No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT19300.12294042931052714No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG19030.12122053729426588No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18860.12013764232106434No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA18340.11682525769715377No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC18300.11657045887992988No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18120.11542386420242236No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17450.11115598401392221No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC17380.1107100860837804No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC17190.10949979170196693No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC17020.10841689672876538No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG16970.10809839820723553No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16930.10784359939001163No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16840.10727030205125787No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC16840.10727030205125787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.030.1454181
GATCTAA555.144245E-420.1818181
GTATATG759.269312E-619.7333341
TGTTACG1002.8765862E-718.516
ATACGGC1002.8765862E-718.529
ATACACA4600.018.49999837
GTATCAA41150.018.3876082
GCAGTCG4650.017.9032259
CAGTCGG4650.017.90322510
CTTATAC19800.017.37878837
GACCGTT1502.5102054E-1017.2666667
CTCTATG7550.016.9072861
TACGAGG1005.8805435E-616.6500027
TTTTACG1005.8805435E-616.6500024
GTATGTA904.4476248E-516.4444451
GGACCGT2150.016.3488376
CCGCTTA1251.6587364E-716.2825
GTCATAT803.3835517E-416.18751
AGGACCG1951.8189894E-1216.1282065
CGTTATT1151.2428263E-616.08695610
Produced by FastQC (version 0.11.5)