##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569866
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.581326686481525	34.0	31.0	34.0	31.0	34.0
2	32.74257420697053	34.0	31.0	34.0	31.0	34.0
3	32.83567450979893	34.0	31.0	34.0	31.0	34.0
4	36.27967673674059	37.0	37.0	37.0	35.0	37.0
5	36.19393757174179	37.0	35.0	37.0	35.0	37.0
6	36.24511391418121	37.0	37.0	37.0	35.0	37.0
7	36.220917581500586	37.0	37.0	37.0	35.0	37.0
8	36.20839549362812	37.0	36.0	37.0	35.0	37.0
9	38.038504560261835	39.0	38.0	39.0	35.0	39.0
10	37.9668111800625	39.0	38.0	39.0	35.0	39.0
11	38.041564057059645	39.0	38.0	39.0	35.0	39.0
12	37.96358861202166	39.0	38.0	39.0	35.0	39.0
13	38.03129502772848	39.0	38.0	39.0	35.0	39.0
14	39.40764944269129	40.0	39.0	41.0	37.0	41.0
15	39.398491336203215	40.0	39.0	41.0	37.0	41.0
16	39.362556422013085	40.0	39.0	41.0	36.0	41.0
17	39.32532458184329	40.0	39.0	41.0	36.0	41.0
18	39.35281036725428	40.0	39.0	41.0	36.0	41.0
19	39.372931829850444	40.0	39.0	41.0	37.0	41.0
20	39.37083929456399	40.0	39.0	41.0	36.0	41.0
21	39.334038701392345	40.0	39.0	41.0	36.0	41.0
22	39.30671280223917	40.0	39.0	41.0	36.0	41.0
23	39.24847534757744	40.0	39.0	41.0	36.0	41.0
24	39.257625173103946	40.0	39.0	41.0	36.0	41.0
25	39.1957676642465	40.0	39.0	41.0	36.0	41.0
26	39.12117403651012	40.0	39.0	41.0	36.0	41.0
27	39.00845422475549	40.0	39.0	41.0	35.0	41.0
28	38.98802509258752	40.0	39.0	41.0	35.0	41.0
29	38.93729719606642	40.0	38.0	41.0	35.0	41.0
30	38.86841934279741	40.0	38.0	41.0	35.0	41.0
31	38.74057085127011	40.0	38.0	41.0	35.0	41.0
32	38.67121716120994	40.0	38.0	41.0	35.0	41.0
33	38.58346572255211	40.0	38.0	41.0	35.0	41.0
34	38.514193568113456	40.0	38.0	41.0	35.0	41.0
35	38.43262163777036	40.0	38.0	41.0	35.0	41.0
36	38.35290082083439	40.0	38.0	41.0	34.0	41.0
37	38.2778765830969	40.0	38.0	41.0	34.0	41.0
38	38.23542582615332	40.0	38.0	41.0	34.0	41.0
39	38.128567024191874	40.0	37.0	41.0	34.0	41.0
40	37.75905905344787	40.0	37.0	41.0	33.0	41.0
41	37.79224723638833	40.0	37.0	41.0	33.0	41.0
42	37.727911172036336	40.0	37.0	41.0	33.0	41.0
43	37.23758333513816	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	2.0
14	2.0
15	2.0
16	3.0
17	3.0
18	4.0
19	17.0
20	62.0
21	126.0
22	244.0
23	498.0
24	807.0
25	1503.0
26	2441.0
27	3878.0
28	6077.0
29	8897.0
30	12620.0
31	17089.0
32	22403.0
33	29987.0
34	42387.0
35	62617.0
36	96465.0
37	186361.0
38	371133.0
39	704235.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.970568188622465	17.041135995046712	12.643690607988198	26.344605208342625
2	19.934886162258437	20.143311594747576	33.835690434725	26.086111808268985
3	21.40545753586612	20.54391903512784	28.880171938241862	29.170451490764183
4	14.725715443228912	16.72894374424314	33.97882367030052	34.566517142227426
5	17.052920440343318	32.86382404612878	32.92975324008546	17.15350227344245
6	33.67376578637922	33.67631377455146	16.14246056669805	16.50745987237127
7	30.460943800298878	28.093607989471714	20.40110429807385	21.04434391215556
8	27.152190059533744	31.447652219998396	20.27421448709635	21.125943233371512
9	27.786957612942757	13.224695610962975	19.034427142189205	39.95391963390506
10	19.036656631839914	24.40596840749465	30.637837879156564	25.91953708150887
11	37.34223175736018	20.73291605780366	19.50395766262853	22.420894522207625
12	22.219921955122285	24.42577901553381	27.46578370383205	25.888515325511857
13	33.801547393217	18.328634418479027	22.591291231226105	25.27852695707786
14	23.85904274632357	20.762217921784405	24.86893785839046	30.509801473501557
15	27.753005670547676	25.36496745582107	22.179408943183684	24.702617930447566
16	25.451853852494416	24.16480132699224	24.04033210477837	26.343012715734975
17	25.388281547597057	24.36475469880869	23.728840550722165	26.51812320287209
18	25.318785170199238	22.03850519725887	25.65531070804769	26.987398924494194
19	27.398707915197857	23.564622713021365	25.370318230982768	23.666351140798003
20	28.170047634638877	22.24380934423702	24.99499957321198	24.591143447912113
21	25.65690320065534	23.620359954289093	24.59674902189104	26.125987823164525
22	27.042371769310247	23.26357791047134	24.184166037101257	25.509884283117156
23	25.187308980511713	23.26606219893927	25.2799920502769	26.26663677027211
24	25.78602250128355	23.377345582361805	24.975953361624494	25.86067855473015
25	25.969413949980446	23.26880128622443	25.646583848557775	25.115200915237352
26	26.40015135049743	23.86222773153887	24.292391834717105	25.445229083246595
27	26.064326509396345	22.97922243044948	25.359935179180898	25.596515880973282
28	25.07602559708918	24.100974223277653	24.73879936249336	26.08420081713981
29	26.037763732700753	24.371124669239286	25.05729788402322	24.53381371403674
30	25.772008566336236	22.955589840151962	25.217120442126905	26.055281151384897
31	25.378599192542552	23.028717100695218	25.336621087404914	26.256062619357323
32	24.003258876872295	23.67928218077212	25.278845455599395	27.038613486756198
33	24.572543134254772	22.731239481586325	26.634502562639106	26.0617148215198
34	25.278972855008007	22.993554863918323	26.258228409303726	25.469243871769947
35	25.164695585483095	23.67603349585251	25.615498392856463	25.543772525807935
36	24.55203182946825	22.94170330461326	25.862016248520575	26.64424861739792
37	24.300736495981184	22.65040455682205	27.20334092209144	25.845518025105328
38	24.047657570773556	21.991494815481065	26.78578936036579	27.175058253379586
39	23.473786934681048	21.793197635976576	28.62237923491559	26.110636194426785
40	24.258630991434938	22.18374052307649	27.990924066130486	25.566704419358082
41	22.312477625478863	22.17781645057604	29.04840285731394	26.461303066631164
42	21.009436474195887	23.21057975648877	29.194338879878917	26.585644889436423
43	21.333859068226204	22.822712256969705	28.467525253747773	27.375903421056318
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	119.5
2	127.0
3	298.5
4	470.0
5	470.0
6	650.5
7	831.0
8	915.0
9	999.0
10	1464.5
11	1930.0
12	1930.0
13	3866.0
14	5802.0
15	10009.0
16	14216.0
17	13829.5
18	13443.0
19	13443.0
20	14921.0
21	16399.0
22	13180.5
23	9962.0
24	9789.0
25	9616.0
26	9616.0
27	9835.0
28	10054.0
29	11771.0
30	13488.0
31	16183.5
32	18879.0
33	18879.0
34	26615.0
35	34351.0
36	36831.5
37	39312.0
38	42935.0
39	46558.0
40	46558.0
41	52613.5
42	58669.0
43	69638.0
44	80607.0
45	97366.5
46	114126.0
47	114126.0
48	122912.0
49	131698.0
50	145237.5
51	158777.0
52	161241.0
53	163705.0
54	163705.0
55	150815.5
56	137926.0
57	122777.0
58	107628.0
59	103494.0
60	99360.0
61	99360.0
62	92945.0
63	86530.0
64	68370.0
65	50210.0
66	45460.5
67	40711.0
68	40711.0
69	36545.5
70	32380.0
71	32633.0
72	32886.0
73	27434.5
74	21983.0
75	21983.0
76	14061.5
77	6140.0
78	5043.0
79	3946.0
80	3249.0
81	2552.0
82	2552.0
83	2084.5
84	1617.0
85	1304.0
86	991.0
87	767.0
88	543.0
89	543.0
90	398.5
91	254.0
92	161.5
93	69.0
94	38.5
95	8.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1569866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.10264558863726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.54183264428875	32.21267486659218
2	7.2572753823019	5.530427836619825
3	2.3634285995430497	2.7015864690731433
4	1.2235674948694732	1.864846344431531
5	0.7698295185292813	1.466627065409623
6	0.5372604517181255	1.2282626748364138
7	0.4048242316516655	1.079741195702106
8	0.2956915840086358	0.9013305303219048
9	0.24885313649590587	0.8533766577171845
>10	1.8664971322279633	14.26283312931399
>50	0.24415800005627963	6.52981705965899
>100	0.20534269667074923	16.046592630894114
>500	0.02998440942923255	7.791942474753063
>1k	0.010949362993820873	6.141436286577914
>5k	5.053552150994249E-4	1.3885047780981148
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7783	0.49577479861338486	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7770	0.4949467024574072	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6080	0.38729420218031346	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2390	0.15224229329127453	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2367	0.15077720009223716	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2184	0.13912015420424417	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2146	0.13669956544061723	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2069	0.13179468820905732	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2008	0.127909006246393	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1930	0.12294042931052714	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1903	0.12122053729426588	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1886	0.12013764232106434	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1834	0.11682525769715377	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1830	0.11657045887992988	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1812	0.11542386420242236	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1745	0.11115598401392221	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	1738	0.1107100860837804	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1719	0.10949979170196693	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1702	0.10841689672876538	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1697	0.10809839820723553	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1693	0.10784359939001163	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1684	0.10727030205125787	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1684	0.10727030205125787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.2739940861194521E-4	0.0	0.0	0.0	0.0
9	1.2739940861194521E-4	0.0	0.0	0.0	6.369970430597261E-5
10	1.9109911291791782E-4	0.0	0.0	0.0	6.369970430597261E-5
11	1.9109911291791782E-4	0.0	0.0	0.0	6.369970430597261E-5
12	1.9109911291791782E-4	0.0	0.0	1.2739940861194521E-4	6.369970430597261E-5
13	2.5479881722389043E-4	0.0	0.0	1.2739940861194521E-4	6.369970430597261E-5
14	2.5479881722389043E-4	0.0	0.0	1.9109911291791782E-4	6.369970430597261E-5
15	3.1849852152986304E-4	0.0	0.0	5.095976344477809E-4	6.369970430597261E-5
16	3.8219822583583564E-4	0.0	0.0	0.001146594677507507	6.369970430597261E-5
17	3.8219822583583564E-4	0.0	0.0	0.0015287929033433426	6.369970430597261E-5
18	5.095976344477809E-4	0.0	0.0	0.001656192311955288	6.369970430597261E-5
19	5.095976344477809E-4	0.0	0.0	0.0023568890593209865	6.369970430597261E-5
20	5.095976344477809E-4	0.0	0.0	0.003057585806686685	6.369970430597261E-5
21	5.732973387537535E-4	0.0	0.0	0.004522679005724056	6.369970430597261E-5
22	6.369970430597261E-4	0.0	0.0	0.00681586836073907	6.369970430597261E-5
23	7.006967473656987E-4	0.0	0.0	0.008663159785612275	6.369970430597261E-5
24	7.006967473656987E-4	0.0	0.0	0.01095634914062729	6.369970430597261E-5
25	7.006967473656987E-4	0.0	0.0	0.012421442339664659	6.369970430597261E-5
26	7.006967473656987E-4	0.0	0.0	0.017453718979836497	6.369970430597261E-5
27	7.006967473656987E-4	0.0	0.0	0.04898507261129294	6.369970430597261E-5
28	7.006967473656987E-4	6.369970430597261E-5	0.0	0.17198920162612605	6.369970430597261E-5
29	7.643964516716713E-4	1.2739940861194521E-4	0.0	0.34633529231157306	6.369970430597261E-5
30	7.643964516716713E-4	1.2739940861194521E-4	0.0	0.5521490369241706	6.369970430597261E-5
31	7.643964516716713E-4	1.2739940861194521E-4	0.0	1.199720230898688	6.369970430597261E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2510	0.0	30.145418	1
GATCTAA	55	5.144245E-4	20.181818	1
GTATATG	75	9.269312E-6	19.733334	1
TGTTACG	100	2.8765862E-7	18.5	16
ATACGGC	100	2.8765862E-7	18.5	29
ATACACA	460	0.0	18.499998	37
GTATCAA	4115	0.0	18.387608	2
GCAGTCG	465	0.0	17.903225	9
CAGTCGG	465	0.0	17.903225	10
CTTATAC	1980	0.0	17.378788	37
GACCGTT	150	2.5102054E-10	17.266666	7
CTCTATG	755	0.0	16.907286	1
TACGAGG	100	5.8805435E-6	16.650002	7
TTTTACG	100	5.8805435E-6	16.650002	4
GTATGTA	90	4.4476248E-5	16.444445	1
GGACCGT	215	0.0	16.348837	6
CCGCTTA	125	1.6587364E-7	16.28	25
GTCATAT	80	3.3835517E-4	16.1875	1
AGGACCG	195	1.8189894E-12	16.128206	5
CGTTATT	115	1.2428263E-6	16.086956	10
>>END_MODULE