FastQCFastQC Report
Fri 10 Feb 2017
ERR1631730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351502
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25010.1850533702502845No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA24220.17920802188971974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.1524230078830812No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20570.15220103262888252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17410.12881963918662348No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16570.12260433206906095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16490.1220123980578645No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16420.12149445579806764No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16120.119274703256081No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14370.10632614676115908No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14280.10566022099856309No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13780.10196063342858537No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13760.10181264992578627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.030.9767421
GTACGAC358.870207E-426.428573
TACGGCT1800.023.6388930
ATACGGC1750.023.25714129
TATAAGG450.003826150420.5555572
CTTATAC13850.020.036137
TGCGGGT7550.018.86754821
ATTACAC500.00703550718.53
GTTAACG701.21939884E-418.513
GTATATC1409.458745E-1118.51
CTATTAC609.23796E-418.51
GTAACGT7300.018.526
GCGGTAA10850.018.41474723
TTCTGCG7900.018.26582318
AACTCCG11400.018.175445
GTATCAA25800.017.9980642
ATAACGC1258.581992E-917.7635
GGTAACG7900.017.56329225
TAATACG10050.017.48756227
CTAACTC11950.017.3389113
Produced by FastQC (version 0.11.5)