FastQCFastQC Report
Fri 10 Feb 2017
ERR1631729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81160
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2160.2661409561360276No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1960.24149827501232135No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1800.22178413011335632No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1540.18974864465253818No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1400.17249876786594381No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1350.16633809758501725No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1330.16387382947264661No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1310.161409561360276No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1280.15771315919172008No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1200.14785608674223755No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1050.12937407589945785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT960.11828486939379004No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA940.11582060128141941No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT920.11335633316904879No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA890.10965993100049286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT880.10842779694430753No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT870.10719566288812223No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT850.1047313947757516No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA830.10226712666338098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1200.033.9166641
AGAGTCA250.00546666429.66
GCTCAAT250.00546666429.634
GGTACTT358.798263E-426.428572
TACTTAG358.798263E-426.428574
GGGTACT358.798263E-426.428571
CTTATAC706.4010237E-926.4285737
GTACTTA358.798263E-426.428573
GTATCAA1650.024.6666682
CTCAATG400.001915845623.12500235
CCCAACA400.001915845623.1250022
TTCATAT400.001915845623.12500211
TTAGATG450.00379570520.5555577
TGGTTCA450.00379570520.5555575
GTTCGCC450.00379570520.55555728
CTCATTA450.00379570520.55555734
GTACTGG450.00379570520.5555571
CTTATTG555.0867087E-420.18181828
GTGTTAG609.136375E-418.51
ACCGGCG500.00698005918.57