FastQCFastQC Report
Fri 10 Feb 2017
ERR1631728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956353
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38440.40194363378375975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36520.38186736487468537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29120.3044900784542946No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19730.20630457582085276No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19390.2027494032015375No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17830.18643743471291457No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16100.168347879914634No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15740.1645835794941826No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15730.1644790155936145No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15610.16322424878679734No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15580.16291055708509306No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15140.15830974546009685No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14010.14649402469590203No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12780.13363266492602627No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12540.13112313131239198No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11400.11920284664762906No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10430.10906014829252378No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10300.10770081758513855No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10050.10508672007093615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG250.00549588729.66
GGTATCA17050.028.2111431
GTGCTAG1001.2891178E-820.351
CCTTATG851.24486E-619.5882361
TAGCGAA951.6753802E-719.47368410
TATACAC3250.018.78461537
GTATCAA25550.018.7534242
GCAGTCG4050.018.7283959
TCGGGTA902.151446E-618.525
CAGTCGG3950.018.26582310
AGTCGGT3950.018.26582311
CTTATAC9550.017.62827137
AACGACC1054.7951835E-717.61904737
CTTATGA852.7223416E-517.4117642
ATACACA2250.017.26666637
GTAGCGA1107.8042285E-716.8181829
CTCTATG6650.016.6917291
GTGATTC4450.016.62921516
GCGGTAA6150.016.54471623
ATACGGC1251.6567355E-716.27999929
Produced by FastQC (version 0.11.5)