##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	956353
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62001478533554	34.0	31.0	34.0	31.0	34.0
2	32.77952178745714	34.0	31.0	34.0	31.0	34.0
3	32.865592516570764	34.0	31.0	34.0	31.0	34.0
4	36.287915654575244	37.0	37.0	37.0	35.0	37.0
5	36.21314305491801	37.0	35.0	37.0	35.0	37.0
6	36.27392291340122	37.0	37.0	37.0	35.0	37.0
7	36.26014871077939	37.0	37.0	37.0	35.0	37.0
8	36.241028155921505	37.0	37.0	37.0	35.0	37.0
9	38.068980805204774	39.0	38.0	39.0	37.0	39.0
10	38.007917578551016	39.0	38.0	39.0	35.0	39.0
11	38.08425131724374	39.0	38.0	39.0	37.0	39.0
12	38.02115536836294	39.0	38.0	39.0	35.0	39.0
13	38.07466594447866	39.0	38.0	39.0	37.0	39.0
14	39.45419421489764	40.0	39.0	41.0	37.0	41.0
15	39.459207008290875	41.0	39.0	41.0	37.0	41.0
16	39.40757021727333	40.0	39.0	41.0	37.0	41.0
17	39.39090168588377	40.0	39.0	41.0	37.0	41.0
18	39.42359463503539	40.0	39.0	41.0	37.0	41.0
19	39.45005139315713	41.0	39.0	41.0	37.0	41.0
20	39.44051516542532	41.0	39.0	41.0	37.0	41.0
21	39.40945864131759	40.0	39.0	41.0	37.0	41.0
22	39.381189790799006	40.0	39.0	41.0	36.0	41.0
23	39.31907778822255	40.0	39.0	41.0	36.0	41.0
24	39.33301301925126	40.0	39.0	41.0	36.0	41.0
25	39.274331758252444	40.0	39.0	41.0	36.0	41.0
26	39.212872234415535	40.0	39.0	41.0	36.0	41.0
27	39.1047489786721	40.0	39.0	41.0	35.0	41.0
28	39.091839519507964	40.0	39.0	41.0	35.0	41.0
29	39.05676565034041	40.0	39.0	41.0	35.0	41.0
30	38.994240620356706	40.0	38.0	41.0	35.0	41.0
31	38.887847897167674	40.0	38.0	41.0	35.0	41.0
32	38.83193548825591	40.0	38.0	41.0	35.0	41.0
33	38.76143536957588	40.0	38.0	41.0	35.0	41.0
34	38.720502785059494	40.0	38.0	41.0	35.0	41.0
35	38.63627656315189	40.0	38.0	41.0	35.0	41.0
36	38.57424507477887	40.0	38.0	41.0	35.0	41.0
37	38.51762581389926	40.0	38.0	41.0	35.0	41.0
38	38.4724646652439	40.0	38.0	41.0	35.0	41.0
39	38.38957267870755	40.0	38.0	41.0	34.0	41.0
40	38.044274446778545	40.0	37.0	41.0	34.0	41.0
41	38.094892785404554	40.0	37.0	41.0	34.0	41.0
42	38.05746622847421	40.0	37.0	41.0	34.0	41.0
43	37.578029242340435	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	0.0
15	1.0
16	2.0
17	3.0
18	8.0
19	7.0
20	22.0
21	79.0
22	115.0
23	252.0
24	446.0
25	809.0
26	1272.0
27	2036.0
28	3262.0
29	4726.0
30	6748.0
31	9188.0
32	12270.0
33	16716.0
34	23906.0
35	35698.0
36	55506.0
37	104052.0
38	223913.0
39	455312.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.40871310070654	17.464158108982772	13.376127852372502	26.75100093793819
2	19.71489606871103	20.35116740366789	34.10393442588668	25.8300021017344
3	20.640182024838108	21.22824940163308	30.201295964983643	27.93027260854517
4	14.818482296808813	16.923980998647988	34.98854502469276	33.26899167985043
5	15.933969988069258	33.87525317534425	34.061795173957734	16.128981662628757
6	31.942912292845843	34.983107701863226	16.350343440131414	16.723636565159516
7	28.860159376297247	29.07963900358968	21.094930428408755	20.965271191704318
8	26.56864149534743	31.839498595183997	20.594696728090987	20.997163181377587
9	27.832191669812296	13.734886595221637	19.071096132913265	39.3618256020528
10	18.879848758774216	24.56843864138033	30.529626612767462	26.022085987077993
11	36.97609564669113	20.970917642334996	20.030365356724975	22.0226213542489
12	22.02596739906708	25.120640600280442	27.688102614829464	25.16528938582302
13	33.34281379365151	18.86709196290491	22.48625768936784	25.303836554075744
14	23.118973851705384	21.522910473433974	24.95312923157035	30.404986443290294
15	28.391503973951043	25.614077647061283	21.71865409529745	24.275764283690226
16	23.981103211889334	25.540360097160775	24.158443587252822	26.32009310369707
17	25.049328020092997	25.18641129373777	23.670757555003224	26.093503131166003
18	25.001124061931108	22.8567275890806	25.546529367294298	26.595618981693995
19	26.359095438608964	23.74562530780998	25.83376640215485	24.0615128514262
20	27.832923617116272	22.2933372928197	25.35580481265809	24.517934277405935
21	25.62578880392491	23.959040228869465	24.35857889294016	26.056592074265467
22	26.158751005120497	24.354500900818003	23.981730595292742	25.505017498768762
23	25.840353927890643	23.699408063758884	25.002378828737925	25.457859179612548
24	26.126440759844954	23.374946280296083	25.034584510112897	25.464028449746067
25	25.78660808299864	23.646185038369723	25.443324797433586	25.12388208119805
26	25.718850675430517	24.38482443198275	24.7566536624029	25.139671230183836
27	25.278950345740537	23.459329348054535	26.022295114879128	25.239425191325797
28	24.521175758323547	24.392562160624788	24.683354368104666	26.402907712947
29	25.35026292592798	25.008338971070305	24.704685403820555	24.93671269918116
30	24.979374770612946	23.792783626966195	26.026268543100716	25.201573059320147
31	25.752938507015717	23.986122279116604	25.152846281655417	25.108092932212266
32	23.82760340585537	24.0493834389603	25.203246081729237	26.91976707345509
33	23.96207258198594	23.577486555696485	26.897181270932386	25.56325959138519
34	24.484787520925853	23.63091870888678	26.056487510364896	25.827806259822474
35	24.092986585497194	25.069717980703775	26.26551074760052	24.571784686198505
36	24.465338635420185	23.994382827261482	26.55808054139005	24.98219799592828
37	24.928243023235144	23.596830877301585	26.114311347379054	25.36061475208422
38	24.17025930801702	23.15452557789854	26.931791922020427	25.743423192064018
39	23.80240350581846	22.389745209143484	27.82068964074981	25.98716164428825
40	23.579996089310118	23.264526801296174	28.139295845780794	25.016181263612914
41	22.07208007921761	22.647599787944408	29.057576020569808	26.22274411226817
42	21.09210720309342	23.07422050226224	29.485765193396162	26.34790710124818
43	20.626379589963122	23.74614812731282	29.044191841297096	26.583280441426965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	25.5
2	30.0
3	99.0
4	168.0
5	168.0
6	246.0
7	324.0
8	338.5
9	353.0
10	554.0
11	755.0
12	755.0
13	1657.0
14	2559.0
15	4878.0
16	7197.0
17	6939.5
18	6682.0
19	6682.0
20	7499.0
21	8316.0
22	7051.0
23	5786.0
24	5928.5
25	6071.0
26	6071.0
27	6515.0
28	6959.0
29	9006.0
30	11053.0
31	12591.5
32	14130.0
33	14130.0
34	16755.0
35	19380.0
36	21296.5
37	23213.0
38	27758.5
39	32304.0
40	32304.0
41	37247.0
42	42190.0
43	46942.0
44	51694.0
45	64562.5
46	77431.0
47	77431.0
48	83982.0
49	90533.0
50	99888.0
51	109243.0
52	107410.5
53	105578.0
54	105578.0
55	93133.5
56	80689.0
57	73770.0
58	66851.0
59	60698.5
60	54546.0
61	54546.0
62	48895.0
63	43244.0
64	35439.5
65	27635.0
66	23887.0
67	20139.0
68	20139.0
69	17297.0
70	14455.0
71	12213.0
72	9971.0
73	8196.5
74	6422.0
75	6422.0
76	5217.5
77	4013.0
78	3312.0
79	2611.0
80	2107.5
81	1604.0
82	1604.0
83	1294.5
84	985.0
85	806.5
86	628.0
87	485.0
88	342.0
89	342.0
90	264.0
91	186.0
92	118.0
93	50.0
94	29.5
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	956353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30936899812131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74258903733399	37.13457427472168
2	6.649989820426998	5.760137259332466
3	2.1862162193583026	2.840509348615993
4	1.1306297345171807	1.9586744148981003
5	0.6932283940307588	1.5011642158526584
6	0.48359078018808294	1.2566406925953033
7	0.34339343961775637	1.0410507231557649
8	0.28814010944375534	0.9983333058446927
9	0.22707528971995586	0.8851038761553184
>10	1.7884931746161175	15.945436647713631
>50	0.26926116109188836	8.096188759702034
>100	0.1789403724800391	14.926609174344014
>500	0.013839350381457576	4.117801320597722
>1k	0.004613116793819192	3.5377759864706273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3844	0.40194363378375975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3652	0.38186736487468537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.3044900784542946	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1973	0.20630457582085276	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1939	0.2027494032015375	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1783	0.18643743471291457	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1610	0.168347879914634	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1574	0.1645835794941826	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1573	0.1644790155936145	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1561	0.16322424878679734	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1558	0.16291055708509306	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1514	0.15830974546009685	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1401	0.14649402469590203	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1278	0.13363266492602627	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1254	0.13112313131239198	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1140	0.11920284664762906	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1043	0.10906014829252378	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1030	0.10770081758513855	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1005	0.10508672007093615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0456390056809568E-4	0.0	0.0	0.0	0.0
8	3.13691701704287E-4	0.0	0.0	0.0	0.0
9	3.13691701704287E-4	0.0	0.0	0.0	0.0
10	3.13691701704287E-4	0.0	0.0	0.0	2.0912780113619135E-4
11	3.13691701704287E-4	0.0	0.0	0.0	2.0912780113619135E-4
12	3.13691701704287E-4	0.0	0.0	1.0456390056809568E-4	2.0912780113619135E-4
13	4.182556022723827E-4	0.0	0.0	1.0456390056809568E-4	2.0912780113619135E-4
14	4.182556022723827E-4	0.0	0.0	1.0456390056809568E-4	2.0912780113619135E-4
15	4.182556022723827E-4	0.0	0.0	2.0912780113619135E-4	2.0912780113619135E-4
16	4.182556022723827E-4	0.0	0.0	5.228195028404783E-4	2.0912780113619135E-4
17	5.228195028404783E-4	0.0	0.0	7.319473039766697E-4	2.0912780113619135E-4
18	6.27383403408574E-4	0.0	0.0	0.0010456390056809567	2.0912780113619135E-4
19	6.27383403408574E-4	0.0	0.0	0.001568458508521435	2.0912780113619135E-4
20	7.319473039766697E-4	0.0	0.0	0.0024049697130662006	2.0912780113619135E-4
21	7.319473039766697E-4	0.0	0.0	0.004391683823860018	2.0912780113619135E-4
22	7.319473039766697E-4	0.0	0.0	0.007633164741470984	2.0912780113619135E-4
23	7.319473039766697E-4	0.0	0.0	0.008887931548288132	2.0912780113619135E-4
24	7.319473039766697E-4	0.0	0.0	0.011188337360786237	2.0912780113619135E-4
25	7.319473039766697E-4	0.0	0.0	0.01254766806817148	2.0912780113619135E-4
26	7.319473039766697E-4	0.0	0.0	0.016102840687486734	2.0912780113619135E-4
27	7.319473039766697E-4	0.0	0.0	0.034401523286903476	2.0912780113619135E-4
28	7.319473039766697E-4	0.0	0.0	0.11711156863626715	2.0912780113619135E-4
29	7.319473039766697E-4	0.0	0.0	0.25210356426967867	2.0912780113619135E-4
30	7.319473039766697E-4	0.0	0.0	0.4275617894229432	2.0912780113619135E-4
31	7.319473039766697E-4	0.0	0.0	0.91807104698788	2.0912780113619135E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACTCG	25	0.005495887	29.6	6
GGTATCA	1705	0.0	28.211143	1
GTGCTAG	100	1.2891178E-8	20.35	1
CCTTATG	85	1.24486E-6	19.588236	1
TAGCGAA	95	1.6753802E-7	19.473684	10
TATACAC	325	0.0	18.784615	37
GTATCAA	2555	0.0	18.753424	2
GCAGTCG	405	0.0	18.728395	9
TCGGGTA	90	2.151446E-6	18.5	25
CAGTCGG	395	0.0	18.265823	10
AGTCGGT	395	0.0	18.265823	11
CTTATAC	955	0.0	17.628271	37
AACGACC	105	4.7951835E-7	17.619047	37
CTTATGA	85	2.7223416E-5	17.411764	2
ATACACA	225	0.0	17.266666	37
GTAGCGA	110	7.8042285E-7	16.818182	9
CTCTATG	665	0.0	16.691729	1
GTGATTC	445	0.0	16.629215	16
GCGGTAA	615	0.0	16.544716	23
ATACGGC	125	1.6567355E-7	16.279999	29
>>END_MODULE