FastQCFastQC Report
Fri 10 Feb 2017
ERR1631727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295224
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5370.18189578083082678No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5030.17037910197002953No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4920.1666531176327128No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4760.16123350405116116No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4210.1426035823645774No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3810.12905454841069833No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3770.12769964501531042No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3740.12668346746876946No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3700.12532856407338155No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3420.1158442403056662No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3310.11211825596834946No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3240.10974717502642063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.10568246484025688No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3020.10229520635178713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG200.001839720937.013
GGTATCA3800.031.1578961
GTGCTAG400.00192780723.1250021
TACGGCT652.6716461E-622.7692330
TGTTGAA502.6955156E-422.220
TCTATGG1201.0913936E-1121.5833342
CCGATAG1151.3278623E-1020.91304427
ACCGATA1151.3278623E-1020.91304426
TCAGGGC450.00381917620.5555573
TAGCACT450.00381917620.5555574
ACGGTAC450.00381917620.5555573
GTTGAAG555.130659E-420.18181821
GTTTATA555.130659E-420.1818181
CGATAGT1101.7389539E-920.18181828
GACCGAT1202.3646862E-1020.04166825
ATACGGC656.879946E-519.92307729
TGGACAA759.227571E-619.7333345
GTATCAA6000.019.7333342
GCAGTCG851.2389046E-619.5882369
CAGTCGG851.2389046E-619.58823610
Produced by FastQC (version 0.11.5)