##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631727.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295224
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.384599490556326	33.0	31.0	34.0	31.0	34.0
2	32.56030336287023	34.0	31.0	34.0	31.0	34.0
3	32.652704387177195	34.0	31.0	34.0	31.0	34.0
4	36.15919098718261	37.0	35.0	37.0	35.0	37.0
5	36.03641641600954	37.0	35.0	37.0	35.0	37.0
6	36.0824899059697	37.0	35.0	37.0	35.0	37.0
7	36.07520052570252	37.0	35.0	37.0	35.0	37.0
8	36.059666558274394	37.0	35.0	37.0	35.0	37.0
9	37.86437755738016	39.0	38.0	39.0	35.0	39.0
10	37.76556445275452	39.0	37.0	39.0	35.0	39.0
11	37.84965653198927	39.0	38.0	39.0	35.0	39.0
12	37.77556363981248	39.0	38.0	39.0	35.0	39.0
13	37.84408787903423	39.0	38.0	39.0	35.0	39.0
14	39.166473592932824	40.0	39.0	41.0	36.0	41.0
15	39.164326071051136	40.0	39.0	41.0	36.0	41.0
16	39.10973362599247	40.0	38.0	41.0	36.0	41.0
17	39.074184348155974	40.0	38.0	41.0	36.0	41.0
18	39.096438636425226	40.0	38.0	41.0	36.0	41.0
19	39.12877679321464	40.0	39.0	41.0	36.0	41.0
20	39.10566552854782	40.0	39.0	41.0	36.0	41.0
21	39.06892393572338	40.0	39.0	41.0	36.0	41.0
22	39.03663658781129	40.0	39.0	41.0	36.0	41.0
23	38.979361434029755	40.0	39.0	41.0	36.0	41.0
24	38.99360485597377	40.0	38.0	41.0	35.0	41.0
25	38.940553613527356	40.0	38.0	41.0	35.0	41.0
26	38.8680662818741	40.0	38.0	41.0	35.0	41.0
27	38.75681855133729	40.0	38.0	41.0	35.0	41.0
28	38.74550172072731	40.0	38.0	41.0	35.0	41.0
29	38.713668265452675	40.0	38.0	41.0	35.0	41.0
30	38.67515174918028	40.0	38.0	41.0	35.0	41.0
31	38.6009403029564	40.0	38.0	41.0	35.0	41.0
32	38.54601590656586	40.0	38.0	41.0	35.0	41.0
33	38.48561431319947	40.0	38.0	41.0	34.0	41.0
34	38.45673793458526	40.0	38.0	41.0	34.0	41.0
35	38.389792157819144	40.0	38.0	41.0	34.0	41.0
36	38.35414803674498	40.0	38.0	41.0	34.0	41.0
37	38.31089274584722	40.0	38.0	41.0	34.0	41.0
38	38.262539630924316	40.0	38.0	41.0	34.0	41.0
39	38.2028188765141	40.0	38.0	41.0	34.0	41.0
40	37.879857328672465	40.0	37.0	41.0	33.0	41.0
41	37.970283581280654	40.0	37.0	41.0	33.0	41.0
42	37.971367503996966	40.0	37.0	41.0	33.0	41.0
43	37.4705342384088	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	1.0
17	1.0
18	7.0
19	6.0
20	20.0
21	32.0
22	67.0
23	122.0
24	193.0
25	313.0
26	487.0
27	765.0
28	1196.0
29	1715.0
30	2367.0
31	3380.0
32	4598.0
33	6162.0
34	8897.0
35	13262.0
36	20184.0
37	35495.0
38	81070.0
39	114881.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.28788987345202	17.097864672248868	13.08159228247026	27.532653171828848
2	20.289000894236242	19.94519415765656	33.611765981085554	26.154038967021652
3	20.9600168008021	20.298823943852803	29.77129230685852	28.969866948486573
4	15.583082676205187	15.968552692193047	34.09614394493673	34.35222068666504
5	16.383491857030595	33.57721594450316	33.841422106603794	16.197870091862452
6	34.16693764734575	33.9142481641059	15.682668075766198	16.23614611278216
7	30.00602932010948	28.35270845188738	20.302888654038966	21.338373573964176
8	26.75798715551581	31.71422377584478	20.575224236511936	20.952564832127468
9	27.886282958025095	13.356637671734006	18.034102918461915	40.72297645177898
10	18.643470720537625	24.232785952361596	30.67806140422188	26.4456819228789
11	38.19032327995014	20.015987860065575	19.645421781426982	22.1482670785573
12	22.694631872747472	24.160298620708343	27.052339918163838	26.092729588380347
13	33.86106820583692	18.12522017180175	21.82207408611766	26.191637536243668
14	23.88389832804921	20.01226187572826	23.73689130964962	32.3669484865729
15	28.76493780993415	24.86688074140314	20.788282795436686	25.579898653226024
16	25.484716689700026	24.652467279083	22.725117199143703	27.137698832073276
17	26.2099287320814	24.231431048966208	22.591320488849146	26.967319730103245
18	25.89525241850256	22.29933880714305	24.25581931008319	27.549589464271197
19	27.55941251388776	22.878898734520227	24.10237650055551	25.459312251036504
20	28.947172316613823	21.609354253041758	23.744343278324255	25.69913015202016
21	26.75019646099233	23.03437389914099	22.609272958838034	27.606156681028644
22	27.42256727095358	23.54212394656261	22.104232718207193	26.931076064276617
23	26.986288377638672	23.139040186434706	23.07027883911877	26.804392596807848
24	27.457117307535974	22.664823998048938	23.147169606807037	26.730889087608055
25	27.39682410644121	22.58285234262797	23.72469717909113	26.29562637183969
26	27.28267349537978	23.191203967157143	23.027599382164052	26.498523155299026
27	26.799989160772835	22.425683548762972	24.177912364848385	26.596414925615804
28	25.953851990353087	23.421876270221933	23.105167601550008	27.519104137874965
29	26.765100398341595	23.88491450559575	23.037083705931767	26.312901390130882
30	26.24752730130342	23.054019998374116	24.278852667804784	26.419600032517682
31	27.283350947077473	22.854849199252094	23.554656802969948	26.30714305070049
32	25.127022193317615	22.986952280302415	23.74264964908002	28.14337587729995
33	25.437972522559143	22.625193073733843	25.057244668455137	26.879589735251873
34	25.805151342709266	22.470056634961928	24.69175947754925	27.033032544779555
35	25.533493211933987	23.70234127306723	24.858751321030812	25.905414193967967
36	25.6049643660407	22.806750128715823	25.44068232934992	26.147603175893558
37	26.249898382245345	22.267159851502587	24.88009104950817	26.602850716743898
38	25.23439828740211	21.958580603202993	26.127957076660437	26.679064032734466
39	24.76932769693521	21.01658401755955	26.999159959894858	27.214928325610384
40	24.499701921253013	21.83426821667615	27.712516597566594	25.95351326450424
41	23.122442619841205	21.21338373573964	28.558992493835188	27.105181150583963
42	22.325420697504267	21.521285532341544	29.062677831070644	27.090615939083545
43	21.41086090561743	21.921320759829825	29.14837547082893	27.519442863723814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	5.5
4	10.0
5	10.0
6	17.0
7	24.0
8	29.5
9	35.0
10	47.5
11	60.0
12	60.0
13	110.0
14	160.0
15	368.5
16	577.0
17	574.5
18	572.0
19	572.0
20	649.0
21	726.0
22	703.5
23	681.0
24	773.5
25	866.0
26	866.0
27	1053.0
28	1240.0
29	1817.0
30	2394.0
31	2846.0
32	3298.0
33	3298.0
34	4113.5
35	4929.0
36	5484.5
37	6040.0
38	7721.5
39	9403.0
40	9403.0
41	10797.5
42	12192.0
43	13463.0
44	14734.0
45	18602.0
46	22470.0
47	22470.0
48	24632.5
49	26795.0
50	29667.0
51	32539.0
52	33648.0
53	34757.0
54	34757.0
55	31112.5
56	27468.0
57	25669.5
58	23871.0
59	21726.5
60	19582.0
61	19582.0
62	17741.5
63	15901.0
64	13207.0
65	10513.0
66	9151.0
67	7789.0
68	7789.0
69	6586.5
70	5384.0
71	4591.0
72	3798.0
73	3160.5
74	2523.0
75	2523.0
76	2025.5
77	1528.0
78	1244.5
79	961.0
80	787.0
81	613.0
82	613.0
83	493.0
84	373.0
85	293.0
86	213.0
87	163.0
88	113.0
89	113.0
90	90.5
91	68.0
92	43.0
93	18.0
94	10.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	295224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.11784193990875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.70671898081504	49.21911792833374
2	5.665227914531613	6.35840729322488
3	1.8519965969747123	3.117901569068274
4	1.012467773983627	2.2727002603865776
5	0.6350716289900393	1.7819424648091702
6	0.449494783769899	1.5134806337047593
7	0.3460688903607082	1.3594447502707285
8	0.2621215549065801	1.176775678983246
9	0.22510877153692105	1.136935661435637
>10	1.6050651518954577	17.75657692330946
>50	0.16466070416105513	6.397202332955193
>100	0.07479094254934372	7.557499137048865
>500	0.001206305524989415	0.3520153664694921
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	537	0.18189578083082678	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	503	0.17037910197002953	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	492	0.1666531176327128	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	476	0.16123350405116116	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	421	0.1426035823645774	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	381	0.12905454841069833	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	377	0.12769964501531042	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	374	0.12668346746876946	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	370	0.12532856407338155	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	342	0.1158442403056662	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	331	0.11211825596834946	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	324	0.10974717502642063	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	312	0.10568246484025688	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	302	0.10229520635178713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.387258488469772E-4	0.0
16	0.0	0.0	0.0	3.387258488469772E-4	0.0
17	0.0	0.0	0.0	3.387258488469772E-4	0.0
18	0.0	0.0	0.0	3.387258488469772E-4	0.0
19	0.0	0.0	0.0	3.387258488469772E-4	0.0
20	0.0	0.0	0.0	6.774516976939544E-4	0.0
21	0.0	0.0	0.0	0.003048532639622795	0.0
22	0.0	0.0	0.0	0.00609706527924559	0.0
23	0.0	0.0	0.0	0.00846814622117443	0.0
24	0.0	0.0	0.0	0.00982304961656234	0.0
25	0.0	0.0	0.0	0.01083922716310327	0.0
26	0.0	0.0	0.0	0.013210308105032112	0.0
27	0.0	0.0	0.0	0.03319513318700377	0.0
28	0.0	0.0	0.0	0.10195648050294014	0.0
29	0.0	0.0	0.0	0.2259301411809338	0.0
30	0.0	0.0	0.0	0.3746307888247568	0.0
31	0.0	0.0	0.0	0.8701867056878845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAG	20	0.0018397209	37.0	13
GGTATCA	380	0.0	31.157896	1
GTGCTAG	40	0.001927807	23.125002	1
TACGGCT	65	2.6716461E-6	22.76923	30
TGTTGAA	50	2.6955156E-4	22.2	20
TCTATGG	120	1.0913936E-11	21.583334	2
CCGATAG	115	1.3278623E-10	20.913044	27
ACCGATA	115	1.3278623E-10	20.913044	26
TCAGGGC	45	0.003819176	20.555557	3
TAGCACT	45	0.003819176	20.555557	4
ACGGTAC	45	0.003819176	20.555557	3
GTTGAAG	55	5.130659E-4	20.181818	21
GTTTATA	55	5.130659E-4	20.181818	1
CGATAGT	110	1.7389539E-9	20.181818	28
GACCGAT	120	2.3646862E-10	20.041668	25
ATACGGC	65	6.879946E-5	19.923077	29
TGGACAA	75	9.227571E-6	19.733334	5
GTATCAA	600	0.0	19.733334	2
GCAGTCG	85	1.2389046E-6	19.588236	9
CAGTCGG	85	1.2389046E-6	19.588236	10
>>END_MODULE