FastQCFastQC Report
Fri 10 Feb 2017
ERR1631726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166301
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2290.13770211844787464No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2220.13349288338614923No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1940.11665594313924751No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1880.11304802737205429No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1870.11244670807752208No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1830.11004143089939328No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1730.10402823795407122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1150.032.1739121
GTATAGG303.5863701E-430.8333321
CTTAGTC250.00548300229.632
CTTTATA358.837401E-426.4285737
GTCGACT400.001924286623.12500212
GTATCAA1600.023.1250022
TCCGTGC1107.2759576E-1121.8636368
CGCGGTA957.034032E-921.42105322
CAGAACT450.003812268420.5555574
TGATAAT450.003812268420.55555716
CTCTGTT450.003812268420.55555712
ATACACA450.003812268420.55555737
TAATACG1101.722583E-920.18181827
GATAATC555.117715E-420.18181817
GGTAATA1101.722583E-920.18181825
CTTATAC1950.019.92307737
CCGCGGT1052.224806E-819.3809521
CTAACTC1152.9958755E-919.3043483
AACTCCG1152.9958755E-919.3043485
GCTAACT1152.9958755E-919.3043482
Produced by FastQC (version 0.11.5)