##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631726.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166301
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.602461801191815	34.0	31.0	34.0	31.0	34.0
2	32.75869658029717	34.0	31.0	34.0	31.0	34.0
3	32.85522035345548	34.0	31.0	34.0	31.0	34.0
4	36.28863326137546	37.0	37.0	37.0	35.0	37.0
5	36.204785298945886	37.0	35.0	37.0	35.0	37.0
6	36.249577573195594	37.0	37.0	37.0	35.0	37.0
7	36.23808636147708	37.0	37.0	37.0	35.0	37.0
8	36.215302373407255	37.0	37.0	37.0	35.0	37.0
9	38.04963890776364	39.0	38.0	39.0	35.0	39.0
10	37.98414922339613	39.0	38.0	39.0	35.0	39.0
11	38.05195398704758	39.0	38.0	39.0	35.0	39.0
12	37.993902622353446	39.0	38.0	39.0	35.0	39.0
13	38.04038460382078	39.0	38.0	39.0	35.0	39.0
14	39.45159680338663	40.0	39.0	41.0	37.0	41.0
15	39.45581806483425	40.0	39.0	41.0	37.0	41.0
16	39.40070715149037	40.0	39.0	41.0	37.0	41.0
17	39.3685906879694	40.0	39.0	41.0	36.0	41.0
18	39.3763657464477	40.0	39.0	41.0	36.0	41.0
19	39.406624133348565	40.0	39.0	41.0	37.0	41.0
20	39.39010589232777	40.0	39.0	41.0	37.0	41.0
21	39.35298645227629	40.0	39.0	41.0	36.0	41.0
22	39.33688913476167	40.0	39.0	41.0	36.0	41.0
23	39.26283666364002	40.0	39.0	41.0	36.0	41.0
24	39.28781546713489	40.0	39.0	41.0	36.0	41.0
25	39.223456263041115	40.0	39.0	41.0	36.0	41.0
26	39.16177894300095	40.0	39.0	41.0	36.0	41.0
27	39.07573015195339	40.0	39.0	41.0	36.0	41.0
28	39.066884745130814	40.0	39.0	41.0	35.0	41.0
29	39.03983740326276	40.0	39.0	41.0	35.0	41.0
30	39.014118977035615	40.0	39.0	41.0	35.0	41.0
31	38.92818443665402	40.0	38.0	41.0	35.0	41.0
32	38.88797421542865	40.0	38.0	41.0	35.0	41.0
33	38.842255909465365	40.0	38.0	41.0	35.0	41.0
34	38.824360647260086	40.0	38.0	41.0	35.0	41.0
35	38.745690043956444	40.0	38.0	41.0	35.0	41.0
36	38.698330136319086	40.0	38.0	41.0	35.0	41.0
37	38.66061539016602	40.0	38.0	41.0	35.0	41.0
38	38.61706183366306	40.0	38.0	41.0	35.0	41.0
39	38.555919687795026	40.0	38.0	41.0	35.0	41.0
40	38.24541644367743	40.0	38.0	41.0	34.0	41.0
41	38.32674487826291	40.0	38.0	41.0	34.0	41.0
42	38.322974606286195	40.0	37.0	41.0	34.0	41.0
43	37.856837902357775	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	4.0
19	3.0
20	8.0
21	18.0
22	32.0
23	68.0
24	100.0
25	134.0
26	205.0
27	292.0
28	514.0
29	740.0
30	1052.0
31	1553.0
32	2128.0
33	2885.0
34	4168.0
35	6173.0
36	9709.0
37	16957.0
38	39512.0
39	80043.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.71291212921149	16.55011094340984	12.76480598432962	27.97217094304905
2	20.53745918545288	19.58436810361934	32.69132476653778	27.186847944389992
3	20.782797457622024	20.028141742984108	29.0449245644945	30.14413623489937
4	16.054022525420773	15.518848353287112	32.69733795948311	35.72979116180901
5	16.776808317448484	33.1940276967667	33.58909447327436	16.440069512510448
6	35.49948587200317	33.00100420322187	15.167677885280305	16.33183203949465
7	31.07798509930788	27.60596749267894	19.874805322878395	21.441242085134785
8	27.559665906999957	31.510333672076534	19.947564957516793	20.982435463406716
9	28.057558282872623	13.221808648174093	17.560928677518476	41.15970439143481
10	18.77439101388446	24.324568102416702	30.60835473027823	26.292686153420604
11	38.63235939651596	19.814673393425174	18.976434296847284	22.576532913211587
12	23.0828437592077	23.66371819772581	26.830866922026924	26.42257112103956
13	34.00580874438518	17.84054214947595	21.744307009579018	26.40934209655985
14	24.529617981852184	19.44786862376053	22.9072585252043	33.115254869182984
15	28.604758840896928	24.668522738889124	20.272277376564183	26.45444104364977
16	26.286672960475283	24.02631373232873	22.04556797613965	27.641445331056335
17	26.683543694866536	23.860349607037843	22.308945827144754	27.147160870950866
18	26.317340244496425	22.0371495060162	23.765942477796283	27.87956777169109
19	28.007648781426447	22.503171959278657	23.440027420159833	26.04915183913506
20	28.92285674770446	21.567519136986547	23.420785202734802	26.088838912574186
21	27.26501945267918	22.695594133528964	22.242800704746212	27.796585709045647
22	28.08100973535938	22.808040841606484	21.45988298326528	27.65106643976885
23	27.329360617194126	22.821269866086194	22.737085164851685	27.112284351868
24	27.75930391278465	22.200107034834428	22.789399943475985	27.25118910890494
25	27.92286276089741	22.29030492901426	23.073222650495186	26.713609659593146
26	27.812821329998016	22.892225542840993	22.678155873987528	26.616797253173463
27	27.141147678005545	22.16282523857343	23.516394970565422	27.179632112855607
28	26.296294069187798	22.91567699532775	22.628246372541355	28.159782562943096
29	26.957745293173225	23.28969759652678	22.73828780344075	27.01426930685925
30	26.626418361885978	23.260834270389232	23.28127912640333	26.83146824132146
31	27.421963788552084	22.859754300936256	22.811047438079147	26.907234472432517
32	25.429792965766897	22.7112284351868	23.32517543490418	28.533803164142128
33	25.86755341218634	22.321573532329932	24.315548312998718	27.49532474248501
34	26.350412805695694	22.08585636887331	23.996849086896653	27.56688173853434
35	25.96075790283883	23.30593321747915	24.33779712689641	26.395511752785612
36	26.102067937053896	22.426804408873068	24.85132380442691	26.61980384964612
37	26.568090390316357	21.701012020372698	24.483917715467737	27.246979873843213
38	25.405740193985604	21.823681156457265	25.637849441675037	27.132729207882093
39	25.15138213239848	20.818876615293956	26.447225212115384	27.582516040192186
40	24.758119313774422	21.584957396527983	27.444813921744306	26.21210936795329
41	23.482119770777086	21.102699322313153	28.155573327881374	27.25960757902839
42	22.699803368590686	21.197106451554713	28.676917156240794	27.42617302361381
43	21.76715714277124	21.39614313804487	28.833861492113698	28.002838227070193
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	2.5
4	4.0
5	4.0
6	7.0
7	10.0
8	8.5
9	7.0
10	20.5
11	34.0
12	34.0
13	63.5
14	93.0
15	168.5
16	244.0
17	252.0
18	260.0
19	260.0
20	277.5
21	295.0
22	309.0
23	323.0
24	401.5
25	480.0
26	480.0
27	563.5
28	647.0
29	957.0
30	1267.0
31	1479.0
32	1691.0
33	1691.0
34	2125.0
35	2559.0
36	2893.0
37	3227.0
38	4084.0
39	4941.0
40	4941.0
41	5692.5
42	6444.0
43	7044.5
44	7645.0
45	9585.5
46	11526.0
47	11526.0
48	12694.5
49	13863.0
50	15567.5
51	17272.0
52	18118.5
53	18965.0
54	18965.0
55	17321.0
56	15677.0
57	14848.0
58	14019.0
59	12791.0
60	11563.0
61	11563.0
62	10736.5
63	9910.0
64	8492.0
65	7074.0
66	6114.5
67	5155.0
68	5155.0
69	4504.0
70	3853.0
71	3315.0
72	2777.0
73	2254.0
74	1731.0
75	1731.0
76	1417.0
77	1103.0
78	917.0
79	731.0
80	553.5
81	376.0
82	376.0
83	328.5
84	281.0
85	207.0
86	133.0
87	101.0
88	69.0
89	69.0
90	53.0
91	37.0
92	22.5
93	8.0
94	6.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	166301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.09839391856915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5482707112074	55.24155272225294
2	6.066236729163353	7.655395894800835
3	2.0402683145304605	3.862129614294544
4	0.9886298888040155	2.495238326537079
5	0.6527285756129143	2.059306239296511
6	0.4298198072765724	1.627256370812438
7	0.30487210401746623	1.346585807985395
8	0.25889138734219835	1.306850459251432
9	0.1959178062711535	1.1125889024183244
>10	1.3934154178415314	16.718477342127123
>50	0.09296099989886723	4.032188691595171
>100	0.027988258034067553	2.542429628628208
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	229	0.13770211844787464	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	222	0.13349288338614923	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	194	0.11665594313924751	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	188	0.11304802737205429	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	187	0.11244670807752208	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	183	0.11004143089939328	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	173	0.10402823795407122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0012026385890644073	0.0
20	0.0	0.0	0.0	0.001803957883596611	0.0
21	0.0	0.0	0.0	0.0030065964726610182	0.0
22	0.0	0.0	0.0	0.0060131929453220365	0.0
23	0.0	0.0	0.0	0.007817150828918647	0.0
24	0.0	0.0	0.0	0.008418470123450851	0.0
25	0.0	0.0	0.0	0.008418470123450851	0.0
26	0.0	0.0	0.0	0.01142506659611187	0.0
27	0.0	0.0	0.0	0.0318699226102068	0.0
28	0.0	0.0	0.0	0.10643351513220005	0.0
29	0.0	0.0	0.0	0.230305289805834	0.0
30	0.0	0.0	0.0	0.40589052380923746	0.0
31	0.0	0.0	0.0	0.8809327664896783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	115	0.0	32.173912	1
GTATAGG	30	3.5863701E-4	30.833332	1
CTTAGTC	25	0.005483002	29.6	32
CTTTATA	35	8.837401E-4	26.42857	37
GTCGACT	40	0.0019242866	23.125002	12
GTATCAA	160	0.0	23.125002	2
TCCGTGC	110	7.2759576E-11	21.863636	8
CGCGGTA	95	7.034032E-9	21.421053	22
CAGAACT	45	0.0038122684	20.555557	4
TGATAAT	45	0.0038122684	20.555557	16
CTCTGTT	45	0.0038122684	20.555557	12
ATACACA	45	0.0038122684	20.555557	37
TAATACG	110	1.722583E-9	20.181818	27
GATAATC	55	5.117715E-4	20.181818	17
GGTAATA	110	1.722583E-9	20.181818	25
CTTATAC	195	0.0	19.923077	37
CCGCGGT	105	2.224806E-8	19.38095	21
CTAACTC	115	2.9958755E-9	19.304348	3
AACTCCG	115	2.9958755E-9	19.304348	5
GCTAACT	115	2.9958755E-9	19.304348	2
>>END_MODULE